A kind of greenhouse industrialized capsicum seedling breeding method
A technology of industrialization and seedlings, which is applied in the field of greenhouse industrialized pepper seedling breeding, can solve the problems of low uniformity of seedling emergence and strong seedling index, high rate of commodity rejection, and affecting the cleanliness of hydroponic boxes, so as to improve the uniformity of seedling emergence and strong seedling index, reducing the rate of commodity survival, and the effect of reducing the incidence of seedlings
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Embodiment 1
[0041] A method for breeding hot pepper seedlings in a greenhouse, comprising the following steps:
[0042] (1) Preparation of 1 / 2MS medium
[0043] 1) Shake 20g of MS base salt and 80g of agarose in 10L of water to prepare a culture medium;
[0044] 2) Adjust the pH value of the culture medium to 5.5, and perform high-temperature sterilization;
[0045] 3) Cool the culture medium down to 58°C naturally, take an appropriate amount and add it to a sterile plate culture dish in a sterile environment, let it stand, and cover the culture dish after the culture medium is solidified to prepare a plate culture medium;
[0046] (2) Seed disinfection
[0047] Specific steps are as follows:
[0048] 1) Take an appropriate amount of seeds in a centrifuge tube, add an appropriate amount of 70% alcohol, and let stand for 1 to 2 minutes;
[0049] 2) Centrifuge the centrifuge tube and remove the supernatant in the tube;
[0050] 3) Add an appropriate amount of a mixture of 1 / 3 sodium hy...
Embodiment 2
[0064] A method for breeding hot pepper seedlings in a greenhouse, comprising the following steps:
[0065] (1) Preparation of 1 / 2MS medium
[0066] 1) Shake 20g of MS base salt and 80g of agarose in 10L of water to prepare the culture medium;
[0067] 2) Adjust the pH value of the culture medium to 5.8, and perform high-temperature sterilization;
[0068] 3) Naturally cool the culture medium to 60°C, and in a sterile environment, take an appropriate amount and add it to a sterile plate culture dish, let it stand, and cover the culture dish after the culture medium is solidified to prepare a plate culture medium;
[0069] (2) Seed disinfection
[0070] Specific steps are as follows:
[0071] 1) Take an appropriate amount of seeds in a centrifuge tube, add an appropriate amount of 70% alcohol, and let stand for 1 to 2 minutes;
[0072] 2) Centrifuge the centrifuge tube and remove the supernatant in the tube;
[0073] 3) Add an appropriate amount of a mixture of 1 / 3 sodium ...
Embodiment 3
[0087] A method for breeding hot pepper seedlings in a greenhouse, comprising the following steps:
[0088] (1) Preparation of 1 / 2MS medium
[0089] 1) Shake 20g of MS base salt and 80g of agarose in 10L of water to prepare the culture medium;
[0090] 2) Adjust the pH value of the culture medium to 6, and perform high-temperature sterilization;
[0091] 3) Naturally cool the culture medium to 62°C, and in a sterile environment, take an appropriate amount and add it to a sterile flat culture dish, let it stand, and cover the culture dish after the culture medium is solidified to prepare a flat culture medium;
[0092] (2) Seed disinfection
[0093] Specific steps are as follows:
[0094] 1) Take an appropriate amount of seeds in a centrifuge tube, add an appropriate amount of 70% alcohol, and let stand for 1 to 2 minutes;
[0095] 2) Centrifuge the centrifuge tube and remove the supernatant in the tube;
[0096] 3) Add an appropriate amount of a mixture of 1 / 3 sodium hypo...
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