Method for improving resistance of sugarcane on smut

A technology for smut and sugarcane whip smut, applied in botany equipment and methods, chemicals for biological control, animal repellants, etc., can solve the problems of pesticide residues, environmental pollution, poor effect, etc. To achieve the effects of long cycle, strong operability, and reduced incidence

Active Publication Date: 2018-12-21
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide the application of soluble silicate in the prevention and treatment of sugarcane smut in order to overcome the disadvantages of poor effect of existing control methods on sugarcane smut, easy to cause environmental pollution and pesticide residues

Method used

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  • Method for improving resistance of sugarcane on smut
  • Method for improving resistance of sugarcane on smut
  • Method for improving resistance of sugarcane on smut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The influence of embodiment 1 sodium silicate on the growth of sugarcane smut

[0060] The present embodiment has studied the influence of sodium silicate of different concentrations on the growth of sugarcane smut, and the concrete experimental steps are as follows:

[0061] 1. Activation of sugarcane smut

[0062] The sugarcane smut was activated with YEPS (16 g / L agarose, 20 g / L sucrose, 20 g / L peptone, 10 g / L yeast extract) solid medium (containing 50 μg / mL ampicillin) for cultivation. In the experiment, the haploid "+" and "-" mating type basidiospores of sugarcane smut were respectively activated to obtain yeast-like haploid basidiospore colonies (such as figure 1 shown).

[0063] 2. Effect of sodium silicate on OD value of sugarcane smut haploid

[0064] Sugarcane smut haploids were cultured in YEPS (20 g / L sucrose, 20 g / L peptone, 10 g / L yeast extract) liquid medium (containing 50 μg / mL ampicillin). The test set 4 levels of sodium silicate 0, 0.15, 0.30, 0.4...

Embodiment 2

[0069] Embodiment 2 A kind of method improving sugarcane to smut resistance

[0070] In this embodiment, a barrel planting test is adopted, and healthy sugarcane stems are inoculated with sugarcane smut by dipping inoculation method, the effective silicon content of the cane planting soil is measured, and different gradient effective silicon content test treatments are set up, and the disease resistance effect of each treatment is analyzed, Determination of disease resistance-related proteins and defense enzyme activities, and agronomic traits of sugarcane.

[0071] 1. Determination of soil available silicon content

[0072] The five-point sampling method was used to sample the plow layer soil of 0-20 cm in the sugarcane experimental field of South China Agricultural University, and the main nutrient content of the soil was determined (the soil contained 0.85 g / kg of total nitrogen, 0.84 g / kg of total phosphorus, 20.53 g / kg of total potassium, alkali Nitrogen solution 79.26mg...

Embodiment 3

[0104] Embodiment 3 A kind of method that improves sugarcane to smut resistance

[0105] In this example, the sugarcane main cultivar ROC22 (high susceptibility to sugarcane smut variety) was used as the material, and the tank planting test was adopted to inoculate healthy sugarcane stems with sugarcane smut by using the dipping inoculation method, and the available silicon content of the cane planting soil was measured. Different gradient effective silicon content test treatments were set up, and the disease resistance effect analysis, disease resistance-related proteins and defensive enzyme activities, and sugarcane agronomic traits were determined for each treatment.

[0106] 1. Determination of soil available silicon content

[0107] The five-point sampling method was used to sample the plow layer soil of 0-20 cm in the sugarcane experimental field of South China Agricultural University to determine the main nutrient content of the soil (soil content of total nitrogen 0.88...

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Abstract

The invention belongs to the technical field of prevention and control on crop diseases and pests, and particularly discloses a method for improving resistance of sugarcane on smut. The method comprises the following steps of before the sugarcane is planted, applying soluble silicate into silicon-lack soil, so that the content of effective silicon in the soil is 230 to 430mg/kg; uniformly mixing water and the soil, enabling the soil to react, and finally planting the sugarcane, so as to obviously reduce the generation rate of sugarcane smut, improve the resistance of the sugarcane on the smut,and promote the growth of the sugarcane. The soluble silicate can be applied to prevent and control the sugarcane shimadae and sugarcane smut. Compared with the traditional disease-resisting, seed-breeding and chemical agent-preventing and controlling methods, the method has the advantages that the safe and non-poisonous effects are realized, the green and environment-friendly effects are realized, and the operability is high; the method can be widely popularized and applied into agricultural production.

Description

technical field [0001] The invention relates to the technical field of crop disease prevention and control, in particular to a method for improving sugarcane resistance to smut. Background technique [0002] Sugarcane smut is a fungal disease caused by sugarcane whip smut (Sporisorium scitamineum, also known as smut). It is one of the most serious sugarcane diseases affecting global sugarcane production, and it has a tendency to increase year by year. Areas cause sugarcane yield losses of up to 50%. Now the disease is prevalent in China's sugarcane areas, seriously affecting the safe production of China's sugarcane industry. [0003] Sugarcane smut can be divided into two diseases: pre-morbidity and onset period. In the early stage of the disease, there are symptoms such as narrow and slender leaves, light green leaf color, small cane stems, and increased tillers; during the disease period, slender, curly black diseased ears emerge from the tips of the sugarcane plants, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/00A01P21/00A01P3/00A01G13/00
CPCA01G13/00A01N59/00
Inventor 沈万宽邓权清李鸿博吴佳
Owner SOUTH CHINA AGRI UNIV
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