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Method for preparing roseolic acid D

A technology of rose acid and rose flower, applied in the field of chemistry, to achieve the effect of efficient separation and purification

Inactive Publication Date: 2018-12-21
淮安安莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no efficient method for the separation and purification of roseic acid A, B, and D

Method used

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  • Method for preparing roseolic acid D
  • Method for preparing roseolic acid D
  • Method for preparing roseolic acid D

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the preparation of rose acid A, B

[0045] 1. Experimental materials

[0046] Double roses are the flowers of double roses, which are freshly picked and dried in the shade for later use.

[0047] DA-201 macroporous resin was purchased from Tianjin Haoju Resin Technology Co., Ltd.

[0048] High-speed countercurrent chromatography TEB300B was purchased from Shanghai Tongtian Biotechnology Co., Ltd.

[0049] 2. Experimental methods and results

[0050] Including the following steps:

[0051] Step S1, extraction: pulverize the double-petal roses dried in the shade, extract with 95% ethanol aqueous solution under heat reflux, the solid-to-liquid ratio is 1:20, extract 3 times, each time for 1.5h, filter, and combine the filtrates to concentrate to nothing mellow;

[0052] Step S2, macroporous resin enrichment: put the extract concentrated to no alcohol smell on the DA-201 macroporous adsorption resin column, the ratio of resin diameter to height is 1:10, a...

Embodiment 2

[0062] Embodiment 2: the preparation of roseic acid D

[0063] 1. Experimental materials

[0064] Double roses are the flowers of double roses, which are freshly picked and dried in the shade for later use.

[0065] DA-201 macroporous resin was purchased from Tianjin Haoju Resin Technology Co., Ltd.

[0066]High-speed countercurrent chromatography TEB300B was purchased from Shanghai Tongtian Biotechnology Co., Ltd.

[0067] 2. Experimental methods and results

[0068] Including the following steps:

[0069] Step S1, extraction: pulverize the double-petal roses dried in the shade, extract with 95% ethanol aqueous solution under heat reflux, the solid-to-liquid ratio is 1:20, extract 3 times, each time for 1.5h, filter, and combine the filtrates to concentrate to nothing mellow;

[0070] Step S2, macroporous resin enrichment: put the extract concentrated to no alcohol smell on the DA-201 macroporous adsorption resin column, the ratio of resin diameter to height is 1:10, and...

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Abstract

The invention discloses a method for preparing roseolic acid D. The method comprises the following steps: S1, performing extraction: crushing rosa rugosa var. plena Rehd dried in shade, performing extraction, performing filtration, and concentrating filtrate until alcohol taste does not exist; step S2, performing macroporous resin enrichment so as to obtain enrichment substance freeze-dried powderrich in roseolic acid D; step S3, by using petroleum ether / ethyl acetate / ethanol / water in the volume ratio being 6 to 5 to 5 to 6 as a solvent system, performing HSCCC separation and purification soas to obtain the roseolic acid D, wherein the purity of the roseolic acid D is 99.3%. Through adoption of the method provided by the invention, the roseolic acid D can be efficiently prepared throughseparation and purification.

Description

technical field [0001] The invention belongs to the field of chemistry and relates to a method for preparing roseic acid A, B and D. Background technique [0002] Roseic acid A, B, and D are two compounds isolated from double-petal roses. The structural formula is as follows: [0003] [0004] Roseic acid A, B, and D are isomers with similar polarities. [0005] Studies have shown that roseic acid A, B, and D have various excellent pharmacological activities, and have the value of being developed into various drugs. However, there is currently no efficient method for separating and purifying roseic acid A, B, and D. Contents of the invention [0006] The object of the present invention is to provide a kind of method for preparing roseic acid A, B and D. [0007] The above object of the present invention is achieved through the following technical solutions: [0008] A method for preparing roseic acid A, B, comprising the steps of: [0009] Step S1, extraction: pulv...

Claims

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Application Information

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IPC IPC(8): C07D493/18
CPCC07D493/18
Inventor 郑嘉荣吕学金区伟平
Owner 淮安安莱生物科技有限公司
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