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Time resolution fluorescent immunochromatographic test strip for detecting flumetralin, and preparation method and application thereof

A time-resolved fluorescence and immunochromatographic test strip technology, applied in analytical materials, biological testing, fluorescence/phosphorescence, etc., can solve the problem of time-resolved fluorescent immunochromatographic test strips that have not yet been available, and achieve increased stability. and fluorescence lifetime, wide application range and good performance

Active Publication Date: 2018-12-21
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, there is no flumetriamine time-resolved fluorescent immunochromatographic test strip on the market

Method used

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  • Time resolution fluorescent immunochromatographic test strip for detecting flumetralin, and preparation method and application thereof
  • Time resolution fluorescent immunochromatographic test strip for detecting flumetralin, and preparation method and application thereof
  • Time resolution fluorescent immunochromatographic test strip for detecting flumetralin, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Composition of Time-Resolved Fluorescent Immunochromatography Test Strips for Detecting Flumetramide

[0040] 1. Test strips

[0041] see figure 1 : The test strip is composed of a bottom plate, a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane and an absorbent pad;

[0042] The sample absorbent pad 1, the conjugate release pad 2, the nitrocellulose membrane 3 and the water absorbent pad 4 are overlapped and pasted on the bottom plate 7 in sequence, and 1 / 3 of the conjugate release pad is covered by the sample absorbent pad from the starting end , the end of the conjugate release pad is connected to the beginning of the nitrocellulose membrane, the end of the nitrocellulose membrane is connected to the beginning of the absorbent pad, the beginning of the sample absorbent pad is aligned with the beginning of the PVC bottom plate, and the end of the absorbent pad is connected to the end of the PVC bottom plate alignment;

[0043] The ...

Embodiment 2

[0045] Example 2 Preparation of Time-Resolved Fluorescent Immunochromatographic Test Strips for Detecting Flumetramide

[0046] The preparation method of the time-resolved fluorescent immunochromatographic test strip for detecting flumethamine mainly comprises the following steps:

[0047] 1) Preparation of the conjugate release pad: Use commercially available fluorescent microspheres to label flumetramine monoclonal antibody and dilute it with a specific buffer system, soak the conjugate release pad in the dilution buffer, and vacuum freeze-dry Post-preparation;

[0048] 2) Preparation of nitrocellulose membrane: Spray the flumethamine hapten-carrier protein conjugate on the detection area of ​​the nitrocellulose membrane to make the detection area; spray goat anti-mouse anti-antibody onto the nitrocellulose membrane The scope of the quality control area above is made into the quality control area;

[0049] 3) Assembling and cutting: Paste sample absorption pads, conjugate ...

Embodiment 3

[0102] Example 3 Application of Time-Resolved Fluorescent Immunochromatography Test Strips for Detecting Flumethionine

[0103] 1. Tobacco sample pretreatment

[0104] Weigh 1.0±0.05 g crushed tobacco sample into a polystyrene centrifuge tube; add 10 mL of 50% aqueous methanol solution, vortex for 3 min with a vortexer, and centrifuge at 6000 rpm for 5 min at room temperature; pipette 100 μL of supernatant and Mix with 400 μL deionized water and wait for detection.

[0105] 2. Test with test strips

[0106] Draw 100 μL of the sample solution to be tested and drop it vertically into the sample hole of the test paper card. When the liquid flows, start timing and react for 10 minutes; insert the test paper card into the carrier of the KFT-100A fluorescence detector, and select the sample to be tested through the touch screen. item, press the "Start Test" button, the fluorescence detector will automatically scan the test paper card, and read or print the test results on the disp...

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Abstract

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting flumetralin, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate, a sample absorbent pad, a combination releasing pad, a nitrocellulose membrane and a water absorbent pad. A flumetralin monoclonal antibody labelled by fluorescent microspheres is embedded into the combination releasing pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A flumetralin hapten-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the quality control area. A flumetralin hapten is obtained in the modes that 2-chlorine-1,3-binitro-5-benzotrifluoride reacts with 3-amidogen-3-(2-chlorine-6-fluorophenyl)-propionic acid to generate 3-(2-chlorine-6-fluorophenyl)-3-(2,6-binitro-4-trifluoromethyl-anilino)-propionic acid, and then the 3-(2-chlorine-6-fluorophenyl)-3-(2,6-binitro-4-trifluoromethyl-anilino)-propionic acid reacts with iodoethane. The time resolution fluorescent immunochromatographic test strip and a detecting method have the advantages that operation is easy, sensitivity is high, the detecting speed is high, and the cost is low, and the flumetralin in large-batch samples can be detected quickly.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, and in particular relates to a time-resolved fluorescent immunochromatographic test strip for detecting flumethamine in tobacco and tobacco products, a preparation method and application thereof. Background technique [0002] Flumetralin is a contact and local systemic dinitroaniline plant growth regulator that inhibits tobacco lateral buds. It is an excellent tobacco bud suppressant. In 1977, it was successfully developed by Ciba-Geigy in Switzerland. In 1990, it was officially registered in my country under the trade name Prime, with the registration number PDll6-90. It is a new type of high-efficiency sprout inhibitor that is popular in the world. It is suitable for flue-cured tobacco, open flame flue-cured tobacco, Maryland tobacco, sun-cured tobacco, and cigar tobacco. Zhejiang Research Institute of Chemical Industry in China was the first to conduct research and development, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/58G01N33/533G01N21/64
CPCG01N21/6408G01N33/533G01N33/558G01N33/582G01N33/585
Inventor 陈黎范子彦刘惠民唐纲岭樊美娟崔华鹏赵乐潘立宁颜权平
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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