A Method for Effectively Improving the Excision Rate of Parathyroid Histopathological Slices in Experimental Animals

A parathyroid and experimental animal technology, applied in the preparation of test samples, sampling devices, etc., can solve problems such as affecting test quality, inability to cut out parathyroid tissue, and loss of parathyroid tissue.

Active Publication Date: 2021-06-22
苏州华测生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the parathyroid gland is too small and its location is irregular, the parathyroid gland tissue often cannot be cut out, resulting in loss of parathyroid gland tissue, which seriously affects the quality of the test

Method used

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  • A Method for Effectively Improving the Excision Rate of Parathyroid Histopathological Slices in Experimental Animals
  • A Method for Effectively Improving the Excision Rate of Parathyroid Histopathological Slices in Experimental Animals
  • A Method for Effectively Improving the Excision Rate of Parathyroid Histopathological Slices in Experimental Animals

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Embodiment 1

[0028] Embodiment 1 hematoxylin staining method

[0029] The fixed thyroid gland was wiped dry with dust-free paper to check the location of the parathyroid glands. The tip of the toothpick was blunted and dipped in hematoxylin solution for staining to stain the parathyroid glands. After the hematoxylin solution was dry, it was dehydrated together with other tissues. When embedding, embed the parts with hematoxylin color on both sides at the same horizontal position. When slicing, the tissue should be roughly trimmed, and carefully observed when it is close to the site marked by hematoxylin, and sliced ​​after the parathyroid glands are trimmed out.

Embodiment 2

[0030] Example 2 Comparative study of hematoxylin-labeled parathyroid glands and traditional film-making methods

[0031] Research method: Thyroid and parathyroid gland tissues of 250 rats were randomly prepared by 5 methods. The production process of the conventional method is as follows: the parathyroid glands together with the thyroid are taken out → 10% neutral formalin fixation → dehydration → paraffin embedding → sectioning → staining. Hematoxylin solution 1-4 labeling method The production process is as follows: take out the parathyroid gland together with the thyroid gland → 10% neutral formalin fixation → label the parathyroid gland with hematoxylin solution 1 → dehydration → paraffin embedding → sectioning → staining; Parathyroid glands were taken out together with the thyroid → 10% neutral formalin fixation → hematoxylin solution 2 labeled parathyroid glands → dehydration → paraffin embedding → sectioning → staining; parathyroid glands were taken out together with t...

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Abstract

The invention discloses a method for effectively improving the cut-out rate of parathyroid histopathology in experimental animals, which comprises the following steps: after the experimental animals are euthanized → dissected → take out the parathyroid together with the thyroid gland → 10% of the neutral Fur Marlin fixation→labeling with hematoxylin solution→dehydration→paraffin embedding→section→staining. Through a comparative study with the traditional production method, it is found that the new production method can increase the rate of animal parathyroidectomy from 50%-70% to 100%, and the method is simple, easy to master, and can be widely applied. The parathyroid glands of experimental animals are prepared to effectively prevent the parathyroid glands from being lost or unable to be cut out during the sectioning process.

Description

technical field [0001] The invention relates to a method for effectively improving the cut-out rate of pathological slices of parathyroid tissue of experimental animals, belonging to the field of new methods of pathological technology. Background technique [0002] In the single-dose and repeated-dose toxicity tests of preclinical safety evaluation of new drugs, the parathyroid gland is the tissue organ that is required to be inspected according to the guidelines issued by the State Drug Administration. Because the parathyroid gland is too small and its location is irregular, it is difficult to cut it out by traditional methods, resulting in the loss of observation records of parathyroid gland slices in some animals. Especially when the target organ of some drugs is the parathyroid gland, the correct judgment of the test results may be affected due to the low rate of parathyroidectomy, which seriously affects the quality of the test and the integrity of the data. [0003] T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/06G01N1/28G01N1/30
CPCG01N1/06G01N1/28G01N1/30
Inventor 陈勇陆国才夏玉叶宗英李萍
Owner 苏州华测生物技术有限公司
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