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Carvacrol solid lipid nanoparticle dispersion with antibacterial activity and its preparation method and application

A technology of solid lipid nanometer and antibacterial activity, which is applied in the field of fungicides, can solve the problems of low absorption rate and bioavailability, darker carvacrol color, and low solubility, so as to increase solubility, inhibit growth, The effect of simple preparation process

Active Publication Date: 2021-03-26
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Carvacrol is easily soluble in ethanol, ether, propylene glycol and alkaline environment, but insoluble in water, resulting in low absorption and bioavailability in the gastrointestinal tract and antibacterial agents
In addition, placing carvacrol in air and light will cause the color of carvacrol to become darker and have poor stability.
The existing carvacrol has the defects of low solubility in water and poor stability, which has become a bottleneck in its application fields such as food, medicine, and antibacterial agents, and needs further improvement

Method used

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  • Carvacrol solid lipid nanoparticle dispersion with antibacterial activity and its preparation method and application
  • Carvacrol solid lipid nanoparticle dispersion with antibacterial activity and its preparation method and application
  • Carvacrol solid lipid nanoparticle dispersion with antibacterial activity and its preparation method and application

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preparation example Construction

[0022] The second aspect of the present invention provides a kind of preparation method of above-mentioned carvacrol solid lipid nanoparticle dispersion liquid with bacteriostatic activity, and this preparation method comprises:

[0023] (1) Melting and mixing carvacrol, glyceryl monostearate and 1,2-propylene glycol ester to obtain an oil phase;

[0024] (2) Mix Tween-80 and part of water evenly to obtain an aqueous phase;

[0025] (3) The oil phase and the water phase are mixed uniformly, and then ethanol is added to obtain a microemulsion;

[0026] (4) Mix the microemulsion with the remaining water evenly to obtain a carvacrol solid lipid nanoparticle dispersion.

[0027] According to the present invention, preferably, in step (1), the melting temperature is 40-80°C.

[0028] According to the present invention, preferably, in step (2), the mixing temperature is 40-80°C.

[0029] According to the present invention, preferably, step (3) is at 40-80°C, rapidly mixing the oi...

Embodiment 1

[0037] The present embodiment provides a carvacrol solid lipid nanoparticle dispersion with antibacterial activity, the specific preparation method is: weigh 4g 1,2-propanediol ester, 4g glyceryl monostearate and 0.4g carvacrol Mix and heat to 70°C until completely melted to obtain a liquid oil phase; additionally weigh 12g of Tween-80 and 200g of distilled water, stir and heat to 70°C to obtain an aqueous phase. Mix the two phases of oil and water rapidly within 0.5min at 70°C, heat the temperature to 80°C and stir for 10 minutes to form a coarse milk, add 40g of absolute ethanol dropwise to the coarse milk to form a clear, transparent and stable microemulsion, The microemulsion was quickly dispersed into 400mL of 2-4°C cold water within 0.5min, and kept stirring for 30 minutes to obtain a carvacrol solid lipid nanoparticle dispersion;

[0038] The carvacrol solid lipid nanoparticle dispersion in Example 1 was placed for 3 months without aggregation and precipitation of nanop...

Embodiment 2

[0040] The present embodiment provides a carvacrol solid lipid nanoparticle with antibacterial activity, the specific preparation method is: take 4g 1,2-propanediol ester, 4g glyceryl monostearate and 0.8g carvacrol and mix and heat to 70°C until completely melted to obtain a liquid oil phase; additionally weigh 12g of Tween-80 and 200g of distilled water, stir and heat to 70°C to obtain an aqueous phase. Mix the two phases of oil and water rapidly within 0.5min at 70°C, heat the temperature to 80°C and stir for 10 minutes to form a coarse milk, add 40g of absolute ethanol dropwise to the coarse milk to form a clear, transparent and stable microemulsion, The microemulsion was quickly dispersed into 400mL of 2-4°C cold water within 0.5min, and kept stirring for 30 minutes to obtain a carvacrol solid lipid nanoparticle dispersion;

[0041] The carvacrol solid lipid nanoparticle dispersion of Example 2 was placed for 3 months without aggregation and precipitation of nanoparticles...

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Abstract

The invention discloses a carvacrol solid lipid nanoparticles dispersion liquid with bacteriostatic activity, a preparation method and application thereof. The carvacrol solid lipid nanoparticles include: carvacrol 0.05-0.5wt%, glycerol monostearate 0.5-1wt%, 1, 2-propylene glycol ester 0.5-1wt%, Twain-80 1-2wt%, ethyl alcohol 5-6wt%, and the remainder is water. The effective component of the carvacrol solid lipid nanoparticles dispersion liquid is derived from the plant extract, which is green, environmentally friendly and harmless to human body. The solid lipid nanoparticles improve the water dispersibility of carvacrol effectively, increase the solubility of carvacrol in water, and improve the stability of carvacrol.

Description

technical field [0001] The invention belongs to the technical field of bactericides, and more specifically relates to a carvacrol solid lipid nanoparticle dispersion with bacteriostatic activity and a preparation method and application thereof. Background technique [0002] Carvacrol, chemical name 5-isopropyl-2-methylphenol, is an isomer of thymol, and its aroma is similar to thymol, so it is also called isothymol. Carvacrol is a monoterpene phenol that is the main constituent of oregano oil and thyme oil. Studies have shown that carvacrol has a wide range of biological activities, such as anti-inflammatory, anti-oxidation and anti-tumor effects. In recent years, studies have shown that carvacrol can inhibit tumor cells such as lung cancer cells, liver cancer cells, breast cancer cells, and cervical cancer cells. proliferation and induce apoptosis. Carvacrol is a safe food additive, which can be used in the production of candies, beverages and chewing gum. As a broad-spe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K9/51A61K47/14A01N31/08A01N25/04A01P1/00
CPCA01N25/04A01N31/08
Inventor 贺军波黄双双韩立娟张维农
Owner WUHAN POLYTECHNIC UNIVERSITY
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