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Special composite biological agent for ginseng and production and preparation method

A technology of compounding biological bacteria and ginseng, which is applied in the directions of biochemical equipment and methods, chemicals for biological control, biocides, etc., to achieve the effect of not producing pathogenic bacteria resistance, having good application prospects and overcoming side effects.

Active Publication Date: 2019-01-04
通化医药高新区恩维泽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, in the prior art, there is no precedent for using microbial agents to prevent and control ginseng red rust. Therefore, those skilled in the art have great room for improvement and technical requirements for the prevention and treatment of ginseng red rust.

Method used

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  • Special composite biological agent for ginseng and production and preparation method
  • Special composite biological agent for ginseng and production and preparation method
  • Special composite biological agent for ginseng and production and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 bacterial agent

[0041] (1) Preparation of Bacillus amyloliquefaciens and Bacillus subtilis bacterial agents

[0042]Purchase A bacteria: Bacillus amyloliquefaciens BNCC188030; B bacteria: Bacillus subtilis BNCC124558.

[0043] (1) Strain activation: Inoculate the BNCC188030 and BNCC124558 strains preserved at low temperature on the LB plate medium, and cultivate them at 30°C for 24 hours; then pick a single colony and inoculate them on the LB slant medium, and cultivate them at 30°C After 24 hours, wash the medium surface with sterile water, and use the eluate as the inoculum;

[0044] (2) Preparation of seed solution: add the inoculation solution prepared in step (1) into the LB liquid medium according to the ratio of 3% (volume percentage), and shake and cultivate at 34° C. for 12-16 hours to obtain the seed solution;

[0045] (3) Fermentation culture: the seed solution obtained in step (2) is inserted into the corn flour bean cake ...

Embodiment 2

[0053] Embodiment 2 Field plot test

[0054] 1. Test material

[0055] The ginseng experimental field is provided by the ginseng planting base of Qinghe Town, Ji'an, and the annual growth is 1-year-old ginseng; a special compound biological agent (fermentation liquid) for ginseng of the present invention is used to prevent and remove ginseng red rust.

[0056] 2. Experimental design

[0057] The 1-year-old ginseng is sprayed (1L A bacterial liquid+1L B bacterial liquid+500g C bacterial agent is mixed with 100 times of water and the whole plant is evenly sprayed), spraying+irrigating (the mixed bacteria The solution was mixed with 100 times of water and sprayed evenly and was irrigated by ground irrigation), and clear water was used as a control. They were applied once every 30 days during the growth period, and applied 3 times in total.

[0058] 3. Field test results

[0059] The morbidity rate of ginseng was investigated by applying compound biological agent for ginseng. ...

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Abstract

The invention provides a special composite biological agent for ginseng and a preparation method, and belongs to the technical field of biological agents. The special composite biological agent for ginseng comprises three composite effective microbial communities including a bacterium A, a bacterium B and a bacterium C, a strain is cultured by a seed solution, is fermented and cultured and then ismixed to obtain the biological agent. By the biological agent, development of a root system of a plant can be promoted, the plant grows with luxuriant foliage, the plant is protected against being infringed by harmful bacteria through the antibacterial property and the disease resistance of beneficial microorganisms, stress resistance of the plant is enhanced, and pest and disease damage is relieved. Therefore, use and residues of chemical fertilizers and pesticide can be relieved, an environment can be repaired effectively, soil is improved, the yield is increased, and the quality is improved.

Description

technical field [0001] The invention relates to a biological bacterial agent, which is a composite biological bacterial agent for removing and preventing ginseng red rust. Background technique [0002] Ginseng belongs to the Araliaceae family, a perennial herb that likes a cool and humid climate, and is a valuable Chinese herbal medicine. Long-term application of chemical fertilizers and pesticides will cause soil compaction, weakened permeability, and easily cause various plant diseases. The reduction of ginseng production has attracted widespread attention. Although chemical pesticides have certain effects in disease prevention, long-term use can not only make crops resistant to pesticides, but also leave residual pollution. The ecological environment is deteriorating day by day. [0003] Ginseng is an extremely precious Chinese medicinal material. Ginseng has always been called the king of all herbs. Changbai Mountain ginseng is a treasure in the ginseng kingdom. [00...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/04A01N63/02A01P3/00C12R1/07C12R1/125C12R1/79
CPCA01N63/10A01N63/30C12N1/14C12N1/20
Inventor 丁健
Owner 通化医药高新区恩维泽生物科技有限公司
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