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Method for micro-stem cutting propagation in Mountain Tai lithospermum erythrorhizon bottles

A cutting propagation and micro-stem technology, which is applied in the directions of vegetative propagation, horticultural methods, botanical equipment and methods, etc., can solve the problem of germplasm utilization, reproduction efficiency, breeding of fine varieties, scale promotion, single reproduction method, etc. Accumulation of diseases and insect pests and other problems, to achieve the effect of overcoming disadvantages and limitations, improving reproductive efficiency, and efficient recycling

Inactive Publication Date: 2019-01-25
TAISHAN RES INST OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The artificial propagation of Taishan Zicao has been carried out for decades, but up to now, Taishan Zicao is mainly propagated by seeds, the propagation method is single, and it is prone to germplasm degradation and accumulation of diseases and insect pests. The planting scale is affected by the quality and quantity of harvested seeds. Therefore, for many years, the use of germplasm, reproductive efficiency, breeding of improved varieties, and scale promotion of Taishan Zicao have been greatly restricted. It is urgent to improve the level of artificial propagation, explore new propagation techniques, and broaden the propagation channels.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Select the Taishan Zicao field seedlings in the growth stage of the Luohanya Forest Farm Experimental Site in Mount Tai, and confirm that there are no diseases and insect pests and strong seedlings. After entering the seed maturity stage in August, the ovoid seeds turn from green to off-white, and the seed coat is hard. 10- 15 seeds, destroy the seed coat, and start seed harvesting after determining that the maturity standard of the seed form has been reached.

[0026] First, the harvested Taishan Zicao seeds are screened. The screening is divided into 6 steps: sieving, water selection (to eliminate disease grains, insect grains and other sundries), washing with water, hand selection, drying, and storage. Among them, the water selection method is: put the poured seeds into a large enough container, put enough water in the container, stir thoroughly, quickly remove the light seeds and impurities floating on the water surface, and immediately remove the sunken seeds and d...

Embodiment 2

[0036] As described in Example 1, the difference is:

[0037] The seedling growth medium used is: MS+3% sucrose;

[0038] The cutting medium is: MS+6-BA 0.2 mg / L+IAA 0.3 mg / L;

[0039] The rooting medium is: 1 / 2MS + IBA 0.01 mg / L + IAA 0.01 mg / L;

Embodiment 3

[0041] As described in Example 1, the difference is:

[0042] The seedling growth medium used is: MS+5% sucrose;

[0043] The cutting medium is: MS+6-BA 1.0 mg / L+IAA 0.8 mg / L;

[0044] The rooting medium is: 1 / 2MS + IBA 0.05 mg / L + IAA 0.06 mg / L;

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Abstract

The invention discloses a method for micro-stem cutting propagation in Mountain Tai lithospermum erythrorhizon bottles. Mountain Tai lithospermum erythrorhizon seeds are artificially selected, seedlings are obtained by adopting a germination accelerating method including the two steps of low-temperature sand treatment and three-layer filter paper plumule culture, stems of the seedlings are used for in-bottle micro-stem cuttage, breeding and rooting are simultaneously performed and are utilized cyclically, organic combination of a tissue culture technology and a cuttage technology is achieved,and a set of whole in-bottle micro-stem cutting propagation system is formed. The method is simple in operation, the technical bottlenecks such as low seed propagation and germination rate, low breeding speed and difficult survival of land seedling cuttage are broken through, high-quality seedlings can be sustainably and quickly obtained, the Mountain Tai lithospermum erythrorhizon propagation efficiency is remarkably improved, Mountain Tai lithospermum erythrorhizon propagation modes are increased, and the problem is effectively solved that Mountain Tai lithospermum erythrorhizon seedlings are limited.

Description

technical field [0001] The invention relates to the field of Taishan comfrey breeding technology in the field of agricultural biotechnology, in particular to a micro-stem cutting propagation method of Taishan comfrey in a bottle. Background technique [0002] Taishan comfrey ( Lithospermum erythrorhizon Sieb. et Zucc. ) is a perennial herbaceous plant belonging to the family Boraginaceae, mostly born in hillside grasslands, and Taishan Zicao is a unique Chinese herbal medicine species in Mount Tai. Comfrey root contains shikonin, which is the main medicinal part of comfrey. It can be used to treat measles, rash, mumps, constipation and other diseases; external use can cure burns and scalds. Due to the special requirements for the growth environment of Mount Tai Zicao, and the excessive harvesting and excavation of people for many years, the wild resources of Mount Tai Zicao are extremely scarce, endangered and in urgent need of protection. In addition, Taishan Zicao is al...

Claims

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Application Information

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IPC IPC(8): A01G2/10A01H4/00
CPCA01H4/001A01H4/008A01G2/10
Inventor 黄艳艳郭伟李国华王玉山赵进红王新花周光锋杨波
Owner TAISHAN RES INST OF FORESTRY
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