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Preparation method and application of berberine hydrochloride composite nanoparticles

A technology of berberine hydrochloride and complexes, which is applied in the field of preparation of berberine hydrochloride complex nanoparticles, can solve the problems of low bioavailability, difficult absorption of berberine hydrochloride, etc., and improve biological activity, economy and society Significant benefits and high encapsulation efficiency

Inactive Publication Date: 2019-01-25
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a preparation method of berberine hydrochloride complex nanoparticles, which can effectively solve the problem that berberine hydrochloride is not easily absorbed in the body, resulting in low bioavailability The problem

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  • Preparation method and application of berberine hydrochloride composite nanoparticles
  • Preparation method and application of berberine hydrochloride composite nanoparticles
  • Preparation method and application of berberine hydrochloride composite nanoparticles

Examples

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Embodiment 1

[0018] Embodiment 1: The preparation method of a kind of berberine hydrochloride complex nanoparticles of the present invention comprises the following steps: (1) Preparation of berberine hydrochloride complex: 10 mg berberine hydrochloride and 30 mg soybean lecithin are dissolved in Put 3 mL of absolute ethanol in a round-bottomed flask, stir in an oil bath at 30°C for 1 h, then remove the organic solvent by rotary evaporation, a thin film is formed on the wall of the round-bottomed flask, that is, the first compound, and the first compound The compound was dissolved in 2ml of chloroform, and centrifuged at 4000r / min for 5min to remove excess and unreacted berberine hydrochloride to obtain a filtrate, which was rotary evaporated and dried in a vacuum oven to obtain the second complex;

[0019] (2) Dissolve 5 mg of the second complex and 75 mg of polyethylene glycol monomethyl ether-polycaprolactone in 2 ml of a mixed solvent made of tetrahydrofuran and ethanol at a volume rati...

Embodiment 2

[0020] Embodiment 2: A preparation method of berberine hydrochloride complex nanoparticles of the present invention, comprising the following steps: (1) Preparation of berberine hydrochloride complex: dissolving 10 mg berberine hydrochloride and 60 mg soybean lecithin in Put 20 mL of absolute ethanol in a round-bottomed flask, stir in an oil bath at 60 °C for 4 hours, and then remove the organic solvent by rotary evaporation. A thin film is formed on the wall of the round-bottomed flask, that is, the first compound, and the first compound The compound was dissolved in 2ml of chloroform, and centrifuged at 4000r / min for 5min to remove excess and unreacted berberine hydrochloride to obtain a filtrate, which was rotary evaporated and dried in a vacuum oven to obtain the second complex;

[0021] (2) Dissolve 5 mg of the second complex and 75 mg of polyethylene glycol monomethyl ether-polycaprolactone in 2 ml of a mixed solvent made of tetrahydrofuran and ethanol at a volume ratio o...

Embodiment 3

[0022] Example 3: A preparation method of berberine hydrochloride complex nanoparticles, comprising the following steps: (1) Preparation of berberine hydrochloride complex: 10 mg berberine hydrochloride and 60 mg soybean lecithin were dissolved in 5 mL In absolute ethanol, place in a round-bottomed flask, stir in an oil bath at 40 °C for 3 h, then remove the organic solvent by rotary evaporation, a thin film is formed on the wall of the round-bottomed flask, that is, the first complex, and the first complex Dissolve in 2ml of chloroform, centrifuge at 4000r / min for 5min, remove excess and unreacted berberine hydrochloride to obtain a filtrate, rotate the filtrate to evaporate, and dry in a vacuum oven to obtain the second complex;

[0023] (2) Dissolve 5 mg of the second complex and 75 mg of polyethylene glycol monomethyl ether-polycaprolactone in 2 ml of a mixed solvent made of tetrahydrofuran and ethanol at a volume ratio of 1:2, and ultrasonically assist the dissolution as a...

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Abstract

A method for preparing berberine hydrochloride composite nanoparticles comprise the following steps: (1) preparing berberine hydrochloride composite nanoparticle; (2) preparing berberine hydrochloridecomposite nanoparticles; as the only active ingredient, the berberine hydrochloride composite nanoparticles prepared by the invention can inhibit MCF-7 cell drugs; the berberine hydrochloride composite nanoparticles prepared by the invention are used as the only active ingredient in preparing anti-staphylococcus aureus and anti-escherichia coli medicines. The above preparation method is simple, the prepared composite nanoparticles have uniform particle size, encapsulation efficiency is high, which can reduce the recognition and phagocytosis of reticuloendothelial phagocytosis system (RES), and has slow release effect, increase the long circulation of carrier in vivo, improve the biological activity of drug, and achieve high efficiency and low toxicity, which provides conditions for the clinical application of berberine hydrochloride, and has significant economic and social benefits.

Description

technical field [0001] The invention relates to medicines, in particular to a preparation method and application of berberine hydrochloride complex nanoparticles. Background technique [0002] Berberine hydrochloride is a kind of isoquinoline alkaloid, which is distributed in many plants of Berberidaceae; at present, many studies have been carried out at home and abroad. Berberine hydrochloride is often used clinically in the treatment of bacterial diarrhea, acute and chronic ulcerative inflammation and other diseases, and it also has certain curative effects on the digestive system, diabetes and tumors. [0003] However, because of its low solubility, it is not easily absorbed in the body, resulting in low bioavailability, which limits the clinical application of berberine hydrochloride. [0004] The nano-drug carrier delivery system has injected new vitality into the research of pharmaceutical preparations. Nanotechnology can wrap fat-soluble drugs in amphiphilic polymer ...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/34A61K47/24A61K31/4375A61P31/04A61P35/00
CPCA61K9/5123A61K9/5146A61K31/4375A61P31/04A61P35/00Y02A50/30
Inventor 李爱荣王改利王瑞郭丽梁成林张振中
Owner ZHENGZHOU UNIV
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