Test reagent and preparation method of cholesterol in high-density lipoprotein

A high-density lipoprotein and cholesterol technology, applied in the field of medical testing, can solve problems such as failure, direct detection, and unsatisfactory stabilization effects, and achieve the effect of enhancing stability and increasing storage time

Inactive Publication Date: 2019-01-29
浙江伊利康生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method is simple, it is not suitable for automatic and large-scale sample detection, and for patients with hypertriglyceridemia, the result of high-density lipoprotein cholesterol will be high, and when the high triglyceride is greater than 5nmol / L, it will cause The Friedwald formula for calculating LDL-C is limited by its
The above-mentioned known methods cannot directly detect on full-automatic, in recent years, directly detect HDL-C reagent on full-automatic also to appear one after another, and the Chinese patent that Chinese authorized announcement number is CN100564539C discloses a kind of assay high The preparation method of the cholesterol reagent in the density lipoprotein and the improvement of the reagent, but the stabilizer that prolongs the storage life of the test reagent is not used in this scheme, the stabilization effect is not ideal, and it will become invalid after a period of storage, so it needs to be improved

Method used

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  • Test reagent and preparation method of cholesterol in high-density lipoprotein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: a kind of assay reagent of cholesterol in high-density lipoprotein, test reagent body, according to parts by weight, also includes stabilizer, the weight ratio of stabilizer and test reagent body is 50:1; Described stabilizer comprises 10 parts of alkanolamide, 4 parts of sodium salicylate, 3 parts of anti-jamming substances, 30 parts of chitosan, 1 part of ion chelating agent, 5 parts of polyoxyethylene oleate, 5 parts of glycerin, 5 parts of 2,4 ,6-Tribromo-3-hydroxybenzoic acid.

[0019] The above-mentioned ion chelating agent is potassium tripolyphosphate, which can be purchased from Lianyungang Dongtai Food Ingredients Co., Ltd.

[0020] The above-mentioned chitosan is modified, and the modification process is that the above-mentioned chitosan is weighed and dissolved in a mixed solution of hydrochloric acid and acetic acid with a volume ratio of 1:1, and the concentration of hydrochloric acid and acetic acid is 5%. , Pour 30 parts of theophylline gr...

Embodiment 2

[0028] Embodiment 2: a kind of assay reagent of cholesterol in high-density lipoprotein, test reagent body, according to parts by weight, also includes stabilizer, the weight ratio of stabilizer and test reagent body is 50:1; Described stabilizer comprises 12 parts of alkanolamide, 5 parts of sodium salicylate, 4 parts of anti-jamming substances, 35 parts of chitosan, 2 parts of ion chelating agent, 5 parts of polyoxyethylene oleate, 5 parts of glycerin, 5 parts of 2,4 ,6-Tribromo-3-hydroxybenzoic acid.

[0029] The above-mentioned ion chelating agent is potassium tripolyphosphate, which can be purchased from Lianyungang Dongtai Food Ingredients Co., Ltd.

[0030] The above-mentioned chitosan is modified, and the modification process is that the above-mentioned chitosan is weighed and dissolved in a mixed solution of hydrochloric acid and acetic acid with a volume ratio of 1:1, and the concentration of hydrochloric acid and acetic acid is 5%. , Pour 35 parts of theophylline g...

Embodiment 3

[0038] Embodiment 3: A reagent for measuring cholesterol in high-density lipoprotein, the test reagent body, according to parts by weight, also includes a stabilizer, and the weight ratio of the stabilizer to the test reagent body is 50:1;

[0039] Described stabilizer comprises 15 parts of alkanolamides, 6 parts of sodium salicylate, 4.5 parts of anti-interference substances, 40 parts of chitosan, 3 parts of ion chelating agents, 5 parts of polyoxyethylene oleate, 5 parts of glycerin, 5 parts 2,4,6-tribromo-3-hydroxybenzoic acid.

[0040] The above-mentioned ion chelating agent is potassium tripolyphosphate, which can be purchased from Lianyungang Dongtai Food Ingredients Co., Ltd.

[0041]The above-mentioned chitosan is modified, and the modification process is that the above-mentioned chitosan is weighed and dissolved in a mixed solution of hydrochloric acid and acetic acid with a volume ratio of 1:1, and the concentration of hydrochloric acid and acetic acid is 5%. , Pour...

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Abstract

The invention relates to a test reagent of cholesterol in high-density lipoprotein. The test reagent comprises a test reagent main body, wherein the test reagent further comprises a stabilizer according to parts by weight, and the weight ratio of the stabilizer to the test reagent main body is 50:1; and the stabilizer comprises 10-20 parts of alkylolamides, 4-8 parts of sodium salicylate, 3-6 parts of anti-interference substance, and 30-50 parts of chitosan. The stability of the test reagent main body is greatly enhanced, thereby prolonging the storage time of the test reagent main body. Because the thickness of the chitosan is large, and the degree of crosslinking of the chitosan is large, so that a dense net structure is formed easily.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a reagent for measuring cholesterol in high-density lipoprotein and a preparation method thereof. Background technique [0002] The negative correlation between the incidence of cardiovascular and cerebrovascular diseases and serum high-density lipoprotein cholesterol (HDL-C) has been confirmed by epidemiology and related clinics. The detection of high-density lipoprotein cholesterol can also be widely used in relevant clinical diagnosis and treatment, and as a One of the risk indicators of abnormal lipid metabolism. Therefore, the accurate determination of high-density lipoprotein cholesterol is very important, especially the lower limit of high-density lipoprotein cholesterol, a very small error may cause a relatively large impact, and the result of high-density lipoprotein cholesterol directly affects low-density lipoprotein cholesterol ( Calculated value of LDL-C)....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92
CPCG01N33/92
Inventor 王贤理邓慰卢强
Owner 浙江伊利康生物技术有限公司
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