Method for specifically detecting H1 influenza A virus subtype by combining RPA (Recombinase Polymerase Amplification) with immunofluorescence chromatography

Abstract

The invention belongs to the field of molecular biological detection, and particularly discloses a method for specifically detecting a H1 influenza A virus subtype by combining RPA (Recombinase Polymerase Amplification) with immunofluorescence chromatography. According to the method, an immunofluorescence chromatographic technique on the basis of an isothermal amplification technique is established by using the detection of an H1 gene of an influenza A virus as an entry point; the isothermal amplification can be carried out at a normal temperature; specific primers (shown by SEQ ID NO. 1 to 2)of the H1 gene only need to be modified respectively and the demand that one reagent is used for detecting the H1 influenza A virus subtype can be realized. An established influenza A virus detectiontechnical platform can be applied to the quick screening of the influenza A virus at a port; the inflow and the outflow of the influenza A virus can be controlled; and the method has important application and popularization value.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, and in particular relates to a method for specifically detecting H1 influenza A subtype by combining RPA with immunofluorescence chromatography. Background technique [0002] With the continuous deepening of economic globalization and the continuous development of international trade, the international flow of vehicles, goods, and personnel has become increasingly frequent, and border ports have become an important way to spread infectious diseases and their vectors. In 2009, the epidemic of H1N1 influenza A caused great harm to the global economy and the health of the population. The experience of influenza A prevention and control suggests that the existing traditional rapid detection technology can no longer meet the needs of quarantine inspection at border ports. Therefore, it is urgent to develop rapid detection methods for influenza A. [0003] Hemagglutination inhibition test (Hm...

AI Technical Summary

Problems solved by technology

The epidemic of influenza A H1N1 in 2009 caused great harm to the global economy and the health of the population. Experience in the prevention and control of influenza A indicates that the existing traditional rapid detection technology can no longer meet the needs of quarantine inspection at border ports. Therefore, it is urgent to develop rapid detection methods for influenza A

However, to use this method for testing, samples need to be sent to regional or central laboratories for testing, and testing cannot be done anytime and anywhere, mainly because this testing method requires professional testing personnel, equipment and complicated operating procedures, and the testing time Long (50-90min), so not suitable for point-of-care testing (POCT)

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