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Construction method and detection method of the uplc characteristic map of the medicinal material of light bamboo leaves

A construction method and a technology of pale bamboo leaves, applied in the field of medicine, can solve the problems of difficult to fully reflect the quality characteristics of pale bamboo leaves, unable to reflect the basic characteristics of traditional Chinese medicine decoction, etc., and achieve the effects of overcoming durability deviation, good separation, and ensuring quality.

Active Publication Date: 2021-10-26
GUANGDONG YIFANG PHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the establishment of the characteristic map of the leaves of the light bamboo reported in the literature is only for the raw materials of the medicinal materials, and the index components are mostly fat-soluble components as the research objects, which are mainly used for the qualitative identification of the authenticity, place of origin, and quality differences of the traditional Chinese medicinal materials, which is difficult to fully reflect The quality characteristics of light bamboo leaves cannot reflect the material basis characteristics of traditional Chinese medicine decoctions

Method used

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  • Construction method and detection method of the uplc characteristic map of the medicinal material of light bamboo leaves
  • Construction method and detection method of the uplc characteristic map of the medicinal material of light bamboo leaves
  • Construction method and detection method of the uplc characteristic map of the medicinal material of light bamboo leaves

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Experimental program
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Effect test

Embodiment 1

[0062] Example 1 The construction of the characteristic map of bamboo leaves medicinal material

[0063] 1. Instruments, reagents and reagents

[0064] The instruments are shown in Table 1, the reagents are shown in Table 2, and the reagents are shown in Table 3:

[0065] Table 1

[0066]

[0067] Table 2

[0068]

[0069] table 3

[0070]

[0071]

[0072] 2. Research object

[0073] In this embodiment, the medicinal materials of dandelion leaves come from 17 batches of samples from 7 authentic or major production areas with large dandelion leaf production in the country, as shown in Table 4 for details:

[0074] Table 4

[0075]

[0076] Remarks: The content of isoorientin is determined according to the content determination method under the item of "Hong Kong Chinese Medicinal Materia Medica" under the item of Elephant Bamboo.

[0077] 3. Preparation of reference substance reference solution, reference medicinal material solution and standard decoction ...

Embodiment 2

[0157] The detection method of embodiment 2 light bamboo leaf medical material

[0158] The present embodiment provides a kind of detection method of light bamboo leaf medical material, and the steps are as follows:

[0159] (1) Chromatographic conditions

[0160] With 4.1 in embodiment 1.

[0161] (2) Preparation of reference substance reference substance solution

[0162] Same as 3 in Example 1.

[0163] (3) Preparation of the sample solution to be tested

[0164] Take 1.0g of the sample to be tested (batch number: DZY18, qualified according to the Chinese Pharmacopoeia), put it in a 50mL volumetric flask, add an appropriate amount of 60% (volume fraction) ethanol aqueous solution, ultrasonicate (power 500W, frequency 40kHz) for 30 minutes, and extract solution, the extract was allowed to cool, and the volume was fixed to the mark with 60% (volume fraction) ethanol aqueous solution, shaken up, filtered, and the subsequent filtrate was taken as the sample solution to be tes...

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Abstract

The invention relates to a construction method and a detection method of UPLC characteristic map of the medicinal material of light bamboo leaves. The characteristic map construction method includes the following steps: using isoorientin and Ridangyao flavin as reference substances to prepare a reference substance reference solution; using light bamboo leaf reference medicinal material to prepare a reference medicinal material solution; taking light bamboo leaf medicinal material and standard soup respectively Add the extraction solvent to extract, filter, and the resulting filtrate is used as the test solution and the standard decoction test solution; draw the reference substance reference solution, the test solution, and the standard decoction test solution respectively, inject Ultra-high performance liquid chromatograph, measure; Compare the obtained test sample spectrum with the standard decoction test sample spectrum, calibrate the characteristic peaks of 4 water-soluble components, and obtain the UPLC characteristic spectrum of the bamboo leaf medicinal material. The characteristic chromatogram can be used for qualitative and quantitative analysis of the quality of the medicinal material Danzhu Leaf, can ensure the quality of the traditional decoction of Danzhu Leaf prepared by using the medicinal material, and is also suitable for detecting other preparations containing Dan Bamboo Leaf.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for constructing and detecting a UPLC characteristic spectrum of a medicinal material of phlox verticillum. Background technique [0002] Bamboo leaves are the dry stems and leaves of grasses Lophatherum gracile Brongn. In summer, it is harvested before flowering and spiked, and dried in the sun. It has the effects of clearing heat and purging fire, relieving restlessness and quenching thirst, diuresis and treating stranguria. Bamboo leaves were first recorded in "Southern Yunnan Materia Medica", and Li Shizhen in the Ming Dynasty said in "Compendium of Materia Medica" that it is sweet, cold, non-toxic, relieves restlessness and heat, facilitates urination, and clears the heart. At present, the known chemical constituents of the leaves of Danzhu are mainly flavonoids, in addition to volatile oils, triterpenoids, phenolic acids, polysaccharides, amino acids, etc. Mode...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/36
CPCG01N30/02G01N30/36
Inventor 王闽予谭吉娇彭致铖张文芳梁浩明胡梅陈向东
Owner GUANGDONG YIFANG PHARMA
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