Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of atg10 Gene as Molecular Marker for Bovine Superovulation Traits

A technology of ATG10 and superovulation, applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as unclear effects and mechanisms

Active Publication Date: 2022-03-04
JILIN UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, some studies have also shown that ATG10 has great changes in oocyte reprogramming, maturation, embryonic inner cell mass development, differentiation, and early fetal development, but its specific role and mechanism are still unclear (He et. al. ,2009; Lee et. al.,2011; Haleet. al.,2017; Wang et. al.,2018)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of atg10 Gene as Molecular Marker for Bovine Superovulation Traits
  • Application of atg10 Gene as Molecular Marker for Bovine Superovulation Traits
  • Application of atg10 Gene as Molecular Marker for Bovine Superovulation Traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] In Hebei Tianhe Beef Cattle Breeding Co., Ltd., 59 individuals to be tested were randomly selected from the cow population to be superovulated, blood was collected for genomic DNA extraction, and PCR amplification was performed using the primers, PCR reaction system and conditions designed in the present invention. Take Takara's 2× Premix Taq TM PCR reaction master mix for PCR amplification. The specific reaction system is: 2× Premix Taq TM 10.0 μl, 0.5 μl each of forward primer and reverse primer (both at a concentration of 10 pmol / μl), 0.5 μl of genomic DNA (including 27 ng DNA), 8.5 μl of distilled water. The PCR reaction conditions were: pre-denaturation at 94°C for 1 minute; denaturation at 94°C for 45 seconds, annealing at 60°C for 45 seconds, extension at 72°C for 45 seconds, a total of 35 cycles; final extension at 72°C for 5 minutes.

[0051] The amplified products were detected by 1.5% agarose gel electrophoresis, and the results showed that they were ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of the ATG10 gene as a molecular marker for bovine superovulation traits, using the extracted bovine genome DNA as a template, designing a pair of specific primers, cloning the partial DNA sequence of the bovine ATG10 gene, and performing sequencing and genotyping And the association analysis between different genotypes and superovulation traits provides molecular markers for marker-assisted selection of cattle; this locus as a molecular marker for cattle reproduction and production performance-assisted selection and its application in genetic improvement provides an important theoretical basis and It has a good application prospect.

Description

technical field [0001] The invention discloses the application of the ATG10 gene as a molecular marker for bovine superovulation traits, specifically relates to the cloning of bovine ATG10 gene fragments and its use in marker-assisted selection of bovine superovulation traits, and belongs to the technical field of animal genetic engineering. Background technique [0002] In recent years, superovulation, artificial insemination and embryo transfer technologies have developed rapidly, especially in the breeding and breeding of beef cattle and dairy cattle, which play an important role in reducing feeding costs, improving individual value for breeding, and quickly obtaining offspring with excellent traits ( Flanders et. al., 2015; Niemann et. al., 2018). [0003] However, although the steps of superovulation and artificial insemination are simple, there are many influencing factors, which can be mainly classified into three categories, namely, individual conditions of livestock...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 张嘉保姜昊袁宝陈承祯张明军王鹏高妍金永勋俞先峰付旭煌闫向民罗丹
Owner JILIN UNIV