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Application of Bupleurum Polysaccharides in Medicines for Treating Myocardial Injury

A technology of Bupleurum polysaccharide and myocardial injury, which is applied in the directions of drug combination, pharmaceutical formula, cardiovascular system diseases, etc., to achieve the effect of reducing myocardial injury

Active Publication Date: 2021-04-27
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of Bupleurum polysaccharide in the treatment of myocardial injury has not been reported in the literature

Method used

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  • Application of Bupleurum Polysaccharides in Medicines for Treating Myocardial Injury
  • Application of Bupleurum Polysaccharides in Medicines for Treating Myocardial Injury
  • Application of Bupleurum Polysaccharides in Medicines for Treating Myocardial Injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Animal experiment: 24 clean-grade male Wistar rats (200±20g), were randomly divided into two groups, 12 rats in each group, and the blank group: intraperitoneal injection of normal saline (1mL / rat) for 3 consecutive days, isopropyl Adrenaline group: Administer ISO (5 mg / kg) for continuous intraperitoneal injection for 3 days.

[0025] 2. Determination of biochemical indicators: After giving the corresponding drugs to the rats in each group, blood samples were taken from the abdominal aorta. 1 ml of whole blood was centrifuged at 3500 rpm for 10 min and stored at -80 °C for analysis of biochemical indicators. Serum LDH and CK-MB levels were measured by an automatic biochemical analyzer, and SOD and MDA levels were evaluated by biochemical indicators.

[0026] 3. Cell culture: H9c2 rat cardiomyocytes: DMEM medium containing 10% fetal bovine serum (FBS), 1% double antibody (penicillin-streptomycin mixed solution), place the cell culture bottle in 5% CO2 in a humidifie...

Embodiment 2

[0032] 1. Animal experiment: 32 clean-grade male Wistar rats (200±20g). Randomly divided into 4 groups, 8 rats in each group, blank group: continuous intraperitoneal injection of normal saline (1 mL / rat) for 3 days, low-dose isoproterenol: continuous intraperitoneal injection of ISO (2.5 mg / kg) for 3 days, isoproterenol Medium-dose epinephrine group: ISO (5 mg / kg) intraperitoneal injection for 3 consecutive days, high-dose isoproterenol group: ISO (7.5 mg / kg) intraperitoneal injection for 3 consecutive days.

[0033] 2. We treated rats with low, medium and high doses of ISO, and the results of Western blot showed the expressions of P53, P-P53, S100-A9, C3, C5 and Caspase-3 in myocardial tissue of ISO low, medium and high groups The level is positively correlated with the dose of ISO ( figure 2 ). The proteomic results were further validated in the H9c2 cardiomyocyte injury model. With the increase of ISO dose, the expressions of P53, P-P53, S100-A9, C3, C5 and Caspase-3 in...

Embodiment 3

[0038] 1. Extraction of Bupleurum polysaccharide: take Bupleurum medicinal material, add 95% ethanol to reflux for defatting twice, and dry it in a ventilated place for later use. Soak in purified water for 30 minutes, reflux to extract, suction filter and combine the filtrate, concentrate, cool, add 95% ethanol to 80% alcohol content, let stand and centrifuge, precipitate and dry to obtain Bupleurum polysaccharide.

[0039] 2. Experiment: 45 clean-grade male Wistar rats, with a body weight of 200±20g, were divided into three groups (blank group, model group, drug administration group) on average, and the rats in the model group were continuously intraperitoneally injected with ISO (5mg / kg) for 3 days; the drug group was administered Bupleurum polysaccharide (10mg / kg) at the same time, and the model group and the blank group were given corresponding administration of the same amount of normal saline, once a day. On the fourth day, the rats in each group underwent abdominal aor...

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Abstract

The invention discloses the application of Bupleurum polysaccharide in the preparation of medicine for treating myocardial injury. During myocardial injury, ROS / P53 / S100‑A9 can form a subtle positive feedback pathway, which acts with C3 during myocardial injury, activates the expression of downstream complement proteins, and activates the complement system to aggravate myocardial injury. The innovation point of the present invention is to start from the above mechanism, apply the complement inhibitory active Bupleurum polysaccharide to the treatment of myocardial injury, and reduce the myocardial injury by inhibiting the complement system activated by the positive feedback pathway. This provides a new treatment method for myocardial injury clinically.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine application, and relates to the application of Bupleurum polysaccharide in the clinical treatment of myocardial injury. Background technique [0002] Isoproterenol (ISO) is a synthetic non-selective β-adrenergic receptor agonist that can excite the heart, increase myocardial contractility, increase heart rate, and relax blood vessels and bronchi. At present, it has been widely used to induce the establishment of myocardial injury models, providing a basis for clinical research on the pathogenesis of cardiovascular diseases. [0003] Reactive oxygen species (ROS) are oxygen derivatives with redox potential, whose pro-oxidative capacity exceeds the antioxidant capacity, manifested as oxidative stress. P53 protein is an important downstream regulatory protein of ROS-induced apoptosis, which is related to DNA damage and repair, cell cycle inhibition and apoptosis. The complement...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P9/00
CPCA61K31/715A61P9/00
Inventor 李遇伯范思邈
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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