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A method for identifying aroma phenotype of rice and its primer pair

A rice and aroma technology, applied in the biological field, can solve the problems of unsuitability for large-scale planting, poor adaptability, and poor agronomic traits.

Active Publication Date: 2022-04-26
HUNAN RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

my country is also rich in fragrant rice resources, such as Qufu fragrant rice, Jiangyong fragrant rice, Yongshun fragrant rice, etc. However, these resources generally have poor agronomic traits, weak stress resistance, poor adaptability, low yield, and are not suitable for large-scale planting , thus unable to meet the domestic market demand

Method used

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  • A method for identifying aroma phenotype of rice and its primer pair
  • A method for identifying aroma phenotype of rice and its primer pair
  • A method for identifying aroma phenotype of rice and its primer pair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1. Development of primers for identification of rice aroma phenotypes

[0048] According to the 8-base deletion and 3-base mutation of the seventh exon of Badh2 gene in rice, the primer set A for rapid detection of aroma genes was designed respectively.

[0049] Among them, the primer set A is composed of three primers whose primer names are badh2-7NF, badh2-7XF and badh2-7R.

[0050] The sequences of badh2-7NF, badh2-7XF and badh2-7R are as follows:

[0051] badh2-7NF: 5'-ACAGGATTCAATCTTAAGAAACTTTTATTGCCCTGGTAAAAAGATTATGGCTTCAG-3' (SEQ ID No.1 in the sequence listing);

[0052] badh2-7XF: 5'-CTGGTATATATTTCAGCTGC-3' (SEQ ID No.2 in the sequence listing);

[0053] badh2-7R: 5'-GAATGATGCTCAAAGTGTC-3' (SEQ ID No.3 in the sequence listing).

Embodiment 2

[0054] Embodiment 2, identification of aroma phenotype of rice variety

[0055] In this example, PCR technology and existing methods are used to identify the aroma phenotype of rice varieties.

[0056] 1, utilize PCR technology to identify rice variety flavor type (method of the present invention)

[0057] 1.1 DNA extraction

[0058] Using the CTAB method, the samples from Nipponbare, 9311, Gumei 4, Shuhui 527, Yuejing Simiao 2, Yuewang Simiao, Yueqisimiao, Teqing, IR64, R900, Huazhan, Jinsui 128, Huanghuazhan , Daohuaxiang No. 2, Ivory Xiangzhan, Yueyou 9113, Nongxiang 18, Dalixiang, Tianyou Huazhan, Jijing 88, Dongdao 2, Xiangwanxian 13, Xiangwanxian 17, Nongxiang 24 Genomic DNA was extracted from the leaves of 30 rice varieties including Nongxiang 32, Taoyouxiangzhan, Xiangwanxian 12, Yuzhenxiang, Y Liangyou 1 and R1128.

[0059] Agarose electrophoresis and Nanodrop2100 were used to detect the quality of the extracted DNA, and it was found that the extracted genomic DNA ...

Embodiment 3

[0075] Example 3, Application of Primer Group A in Identification of Aroma Rice and Non-Aromatic Rice Materials

[0076] 94 accessions of rice were F hybrids of parents Xiangwanxian 13 and Gumei 3 The specific method is as follows: use Gumei 4 as the male parent and Xiangwanxian 13 as the female parent to carry out artificial crosses to obtain F 1 generation; planting F 1 , to harvest the seeds, that is, to obtain F 2 ; then plant F 2 generation, harvest seeds from individual plants, and obtain F 3 generation groups.

[0077] DNA was extracted from 94 rice materials by CTAB method. The primer set in Example 1 was used to perform PCR amplification on 94 parts of rice materials. The amplification system and amplification conditions are the same as the conditions for amplifying 30 rice varieties with primer set A in Example 2. The same method as in Example 2 was used to detect the PCR amplification product.

[0078] The fragrance of leaves and grains was identified by the...

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Abstract

The invention discloses a method for identifying aroma phenotype of rice and a primer pair used therefor. The method for identifying or assisting the identification of aroma-type rice provided by the present invention includes the following steps: using the genomic DNA of the rice to be identified as a template, using the single-stranded DNA molecule shown in SEQ ID No.1 and the molecule of SEQ ID No.2 The single-stranded DNA shown is the forward primer, and the single-stranded DNA molecule shown in SEQ ID No. 3 is used as the reverse primer for amplification, and the size of the amplified product is detected, and the size of the amplified product is determined according to the following method Whether the rice to be identified is fragrant rice: if the amplified product of the rice to be identified is only a DNA fragment with a size of 167bp, the rice to be identified is fragrant rice or candidate fragrant rice; if the rice to be identified is fragrant rice or a candidate fragrant rice; If the amplified product of the rice to be identified contains a DNA fragment with a size of 206 bp, the rice to be identified is a non-aromatic rice or a candidate non-aromatic rice.

Description

technical field [0001] The invention relates to a method for identifying aroma phenotypes of rice and a pair of primers used in the field of biotechnology. Background technique [0002] Rice is an important food crop in my country and the world. With the development of the economy and the improvement of living standards, people's demand for rice is no longer satisfied with the quantity, but has put forward higher requirements for the quality. Fragrance is an important trait in high-quality rice. Rice varieties with fragrance have a fragrant rice smell and are deeply loved by consumers, so the price is high and the market demand is strong. At present, basmati rice is very popular in the international rice market. The main varieties are Basmati series in India, KDML105 in Thailand and Della in the United States. my country is also rich in fragrant rice resources, such as Qufu fragrant rice, Jiangyong fragrant rice, Yongshun fragrant rice, etc. However, these resources genera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11A01H1/04
CPCA01H1/04C12Q1/6858C12Q1/6895C12Q2600/156C12Q2600/13C12Q2531/113C12Q2565/125
Inventor 胡文彬周政赵正洪周斌张世辉李咏谊
Owner HUNAN RICE RES INST
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