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Establishing method of cataract model for live dogs

A method for establishing a cataract and cataract technology, which is applied in the field of establishing cataract models in living dogs, can solve the problems of great harm, complex influencing factors, unknown success rate and influencing factors, etc., and achieves a simple method, good repeatability and convenient drug source. Effect

Inactive Publication Date: 2019-03-29
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0005] As a kind of oxygen free radical, H 2 o 2 It is a more harmful one, among which there is the use of H 2 o 2 There are reports on the establishment of an isolated cataract model, but compared with the in vitro environment, the influencing factors are more complicated. Currently, the H 2 o 2 There is no report on the establishment of cataract models in living dogs, and its success rate and influencing factors are still unknown, and further research is needed

Method used

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  • Establishing method of cataract model for live dogs
  • Establishing method of cataract model for live dogs

Examples

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Embodiment 1

[0025] In the following examples, dogs of multiple breeds can be selected for testing, and the laboratory selected poodles to carry out the following testing process.

[0026] 1. Experiment group processing

[0027] 14 healthy poodles (12-18 months old, weight 5-6.5kg) were randomly divided into 7 groups, two in each group, and 4 eyes were used for the test. Among them, 1 blank control group (group 1) and 6 experimental groups (group 2-7).

[0028] 2. General and eye examination

[0029] General and ophthalmological examinations of the experimental dogs were performed prior to the experiment. The systemic examinations carried out include body temperature, respiration, heartbeat, blood pressure, blood sugar, and the presence or absence of obvious trauma to the naked eye.

[0030] Eye examinations performed include general examinations and visual acuity tests. General inspection includes palpation to see if the eyeball of the experimental animal is normal and whether there i...

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Abstract

The invention discloses an establishing method of a cataract model for live dogs. The method comprises the following steps of selecting a healthy live dog, performing pupil dilating with atropine sulfate on the day before model making, performing pupil dilating once again with the atropine sulfate before model making, performing general anaesthesia on the live dog before model making, then performing needle insertion along the edge of corneosclera, penetrating anterior lens capsules through the pupil, injecting 0.12-0.18mL of 3% hydrogen peroxide into crystalline lenses in multiple points, andafter 10 days, successfully constructing the cataract model for the live dog. According to the method disclosed by the invention, an H2O2 solution having specific dosage and concentration is injectedinto the crystalline lenses of the live dog, and the cataract model is successfully established in the live dog. The method is simple, high in success rate, high in stability and good in repeatability; and medicines adopted by the method are convenient in source, low in price and free form toxins, and an ideal cataract model of an animal live body is provided for development of clinical ophthalmology department of pets.

Description

technical field [0001] The present invention relates to the technical field of animal models, and more particularly, to a method for establishing a live dog cataract model. Background technique [0002] The development of domestic pet ophthalmology is inseparable from the establishment of pathological models. The successful establishment of the cataract pathological model can provide veterinarians with practical clinical experience in dealing with the disease and the theoretical basis for cataract disease. Pathological model. [0003] The current research on cataract believes that one of the important pathogenesis of cataract is oxidative damage, which is essentially oxidative stress. Among the many types of cataracts, the most common is senile cataract. Because with the decline of the body's antioxidant capacity, the lens epithelial cells accumulate a large number of oxygen free radicals, and the conformation of proteins is cross-linked or accumulated, causing oxidative ...

Claims

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Application Information

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IPC IPC(8): A61K33/40A61K31/46
CPCA61K31/46A61K33/40
Inventor 刘翠谭盈石达友姚丽丽
Owner SOUTH CHINA AGRI UNIV
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