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Tissue culture method of Clerodendrum inerme

A tissue culture and Kulang tree technology, applied in the field of plant tissue culture, can solve the problems of difficulty in large-scale seedling raising, low reproduction speed and high cost

Active Publication Date: 2019-04-02
惠州市林业科学研究所(广东省林业区域性试验(惠州)中心)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, due to the unique habitat characteristics of Kulang tree growing near water, it mostly grows on coastal beaches and places where the tide can reach. After the seeds mature, they will fall into the water and be washed away by the current, which makes it difficult to collect seeds, and the conventional seed breeding methods are limited. Seasonal restrictions make it difficult to grow seedlings on a large scale
On the other hand, the cost of adopting the method for raising seedlings of Kulang tree is high, the propagation speed is low, and it is impossible to raise seedlings quickly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Disinfection of explants

[0049] For more than 3 consecutive sunny days, select the semi-lignified branches of the seedlings of Kulang tree with strong growth and good growth, put them into sealed bags, and bring them back to the laboratory for pretreatment. Firstly, the selected branches of Kulang tree were rinsed under running water for 2 hours, then put into an ultrasonic cleaning machine and cleaned with 0.2% sterilized net for 20 minutes, and finally rinsed with sterile water for 5 times for later use. Disinfect with 75% alcohol by volume on the ultra-clean workbench for 30 seconds, then disinfect with 0.1% (W / V) mercuric chloride for 11 minutes, rinse with sterile water for 5 times, and blot dry with sterile filter paper spare.

[0050] (2) Induction culture

[0051] The semi-lignified branch after processing in (1) is intercepted into the stem section of 2cm with the scissors after sterilization, each stem section has a bud point, inserts in the induction ...

Embodiment 2

[0061] (1) Disinfection of explants

[0062] For more than 3 consecutive sunny days, select the semi-lignified branches of the seedlings of Kulang tree with strong growth and good growth, put them into sealed bags, and bring them back to the laboratory for pretreatment. Firstly, the selected branches of Kulang tree were rinsed under running water for 2 hours, then put into an ultrasonic cleaning machine and cleaned with 0.2% sterilized net for 20 minutes, and finally rinsed with sterile water for 5 times for later use. Disinfect with 75% alcohol by volume on the ultra-clean workbench for 45 seconds, then disinfect with 0.1% (W / V) mercuric chloride for 8 minutes, rinse with sterile water for 5 times, and blot dry with sterile filter paper spare.

[0063] (2) Induction culture

[0064] The semi-lignified branches treated in (1) are cut into 2cm stem sections with sterilized scissors, each stem section has a bud point, and are inserted into the induction medium for cultivation,...

Embodiment 3

[0073] (1) Disinfection of explants

[0074]For more than 3 consecutive sunny days, select the semi-lignified branches of the seedlings of Kulang tree with strong growth and good growth, put them into sealed bags, and bring them back to the laboratory for pretreatment. Firstly, the selected branches of Kulang tree were rinsed under running water for 2 hours, then put into an ultrasonic cleaning machine and cleaned with 0.2% sterilized net for 20 minutes, and finally rinsed with sterile water for 5 times for later use. Disinfect with 75% alcohol by volume on the ultra-clean workbench for 60 seconds, then disinfect with 0.1% (W / V) mercuric chloride for 5 minutes, rinse with sterile water for 5 times, and blot dry with sterile filter paper spare.

[0075] (2) Induction culture

[0076] The semi-lignified branches treated in (1) are cut into 2cm stem sections with sterilized scissors, each stem section has a bud point, and are inserted into the induction medium for cultivation, ...

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Abstract

A tissue culture method of Clerodendrum inerme includes the steps of disinfecting an explant, wherein the explant is a semi-lignified twig isolated from a Clerodendrum inerme seedling; cutting the explant into stem segments 1.0-2.5 cm in length, wherein each stem segment carries one bud point; inoculating the stem segments to an induced culture medium to carry out induced culture for 12-45 days toobtain cluster buds; dividing the cluster buds into a plurality of plantlets, and inoculating the plantlets to an enrichment medium to carry out enrichment culture for 38-45 days to obtain secondarybuds; cutting the secondary buds into single-bud stem segments with a top bud; inoculating the single-bud stem segments to a rooting medium to carry out rooting culture to obtain rooted seedlings. Thetissue culture method of Clerodendrum inerme has the advantages that massive seedlings can be attained quickly, tissue culture is not limited to seasons, the production requirement on its demand canbe met, seedling growth cost can be reduced for Clerodendrum inerme seedlings, production efficiency can be improved, and the method herein is suitable for large-scale seedling growth.

Description

technical field [0001] The present application relates to the technical field of plant tissue culture, in particular to a tissue culture method of Kulang tree. Background technique [0002] Clerodendrum inerme belongs to the genus Clerodendrum of Verbenaceae (Verbenaceae). The branches, leaves, and roots of Kulang tree can be used as medicine, and are often used to treat many different diseases. In addition, it is also a commonly used bitter tonic and can also be used as a coastal sand prevention tree species. [0003] Yet there are many problems in the seedling raising of existing Kulangshu. On the one hand, due to the unique habitat characteristics of Kulang tree growing near water, it mostly grows on coastal beaches and places where the tide can reach. After the seeds mature, they will fall into the water and be washed away by the current, which makes it difficult to collect seeds, and the conventional seed breeding methods are limited. Seasonal restrictions make it di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘德浩徐平陈智涛舒夏竺廖文莉郑洲翔
Owner 惠州市林业科学研究所(广东省林业区域性试验(惠州)中心)
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