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Interiorlabels for distinguishing and correcting high-throughput sequencing contamination and application thereof

A high-throughput, internal standard technology, applied in the field of in vitro diagnosis and high-throughput sequencing, can solve problems such as pollution and achieve suitable designability

Inactive Publication Date: 2019-04-16
SHANGHAI ACEBIOX BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Specifically, the present invention overcomes the pollution problem in high-throughput sequencing through the following technical solutions

Method used

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  • Interiorlabels for distinguishing and correcting high-throughput sequencing contamination and application thereof
  • Interiorlabels for distinguishing and correcting high-throughput sequencing contamination and application thereof
  • Interiorlabels for distinguishing and correcting high-throughput sequencing contamination and application thereof

Examples

Experimental program
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Embodiment

[0109] The technical solutions of the present invention will be described in detail below in conjunction with the drawings and embodiments, but the present invention is not limited to the scope of the embodiments.

[0110] For the experimental methods that do not specify specific conditions in the following examples, select according to conventional methods and conditions, or according to the product instructions. The reagents and raw materials used in the present invention are all commercially available.

[0111] 1. Detect index pollution in the factory or preparation process

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Abstract

The invention provides interiorlabels for distinguishing and correcting high-throughput sequencing contamination and application thereof. The interior labels of DNA or RNA segments with the length oftens to hundreds of basic groups or basic group pairs, and each sample corresponds to different interior labels. The interior labels can precisely and reliably distinguish and correct the contamination in high-throughput sequencing be used for the basic group balancing in sequencing and tracking the error rate in the PCR and sequencing process.

Description

technical field [0001] The present invention belongs to the field of high-throughput sequencing, in particular, belongs to the field of in vitro diagnostic technology, and relates to an internal standard for discriminating and calibrating high-throughput sequencing contamination and a method for discriminating and calibrating high-throughput sequencing contamination using the internal standard. and various other uses. Background technique [0002] High-throughput sequencing (NGS) can sequence hundreds of thousands to millions of DNA molecules in parallel at a time. Reversible terminal termination sequencing, semiconductor sequencing, pyrosequencing, etc. all belong to high-throughput sequencing. [0003] The basis of high-throughput sequencing is to construct a sequencing library of samples. According to the sequencing object, it is divided into DNA library and RNA library. DNA library includes but not limited to whole genome library, whole exome library, targeted DNA libra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869C12N15/11
CPCC12Q1/6869C12Q2600/166C12Q2535/122
Inventor 雷向东邓丽盈
Owner SHANGHAI ACEBIOX BIOTECHNOLOGY CO LTD