Interiorlabels for distinguishing and correcting high-throughput sequencing contamination and application thereof

Abstract

The invention provides interiorlabels for distinguishing and correcting high-throughput sequencing contamination and application thereof. The interior labels of DNA or RNA segments with the length oftens to hundreds of basic groups or basic group pairs, and each sample corresponds to different interior labels. The interior labels can precisely and reliably distinguish and correct the contamination in high-throughput sequencing be used for the basic group balancing in sequencing and tracking the error rate in the PCR and sequencing process.

Description

technical field [0001] The present invention belongs to the field of high-throughput sequencing, in particular, belongs to the field of in vitro diagnostic technology, and relates to an internal standard for discriminating and calibrating high-throughput sequencing contamination and a method for discriminating and calibrating high-throughput sequencing contamination using the internal standard. and various other uses. Background technique [0002] High-throughput sequencing (NGS) can sequence hundreds of thousands to millions of DNA molecules in parallel at a time. Reversible terminal termination sequencing, semiconductor sequencing, pyrosequencing, etc. all belong to high-throughput sequencing. [0003] The basis of high-throughput sequencing is to construct a sequencing library of samples. According to the sequencing object, it is divided into DNA library and RNA library. DNA library includes but not limited to whole genome library, whole exome library, targeted DNA libra...

AI Technical Summary

Problems solved by technology

Specifically, the present invention overcomes the pollution problem in high-throughput sequencing through the following technical solutions

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