Micro-examination flaking method of rhizoma gastrodiae powder and application of method

A technology of microscopic observation and gastrodia elata, applied in the field of microscopic technology, can solve the problems of not being able to truly reflect the effect of ultrafine pulverization, and not having a clear wall-breaking rate, etc., and achieve the effect of high accuracy and scientific calculation.

Active Publication Date: 2019-04-26
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The patent with the publication number CN107802746A discloses "Gastrodia elata broken wall decoction pieces and its preparation method". This patent limits the unbroken wall limit of Gastrodia elata broken wall decoction pieces to 0-5, and does not specify the wall breaking rate, which cannot truly reflect the ultrafine pulverization Effect

Method used

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  • Micro-examination flaking method of rhizoma gastrodiae powder and application of method
  • Micro-examination flaking method of rhizoma gastrodiae powder and application of method

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Embodiment 1

[0028] Utilize the method of the present invention to observe and count the linear relationship between the quantity and the quality of the parenchyma cells of Gastrodia elata powder, comprising the following steps:

[0029] (1) Take 30 mesh Gastrodia elata coarse powder, place it in a volumetric flask and add 1mol / L hydrochloric acid, remove the white milky liquid dissolved by hydrochloric acid, repeat several times until the added hydrochloric acid is in a clear state, remove hydrochloric acid, and use a small amount of After washing with 0.05mol / L NaOH, use distilled water to wash the Gastrodia elata coarse powder;

[0030] (2) add the ethanol-glycerol solution with a volume ratio of 5:1 to suspend the Gastrodia elata coarse powder, the mass volume ratio of the gastrodia elata coarse powder and the ethanol-glycerin solution at a constant volume is 1:20, fully shake the gastrodia elata coarse powder suspension, Use a pipette gun to draw 6 suspensions of different volumes ont...

Embodiment 2

[0037] Utilize the method of the present invention to observe and count the linear relationship between the quantity and the quality of the parenchyma cells of Gastrodia elata powder, comprising the following steps:

[0038] (1) Take 100 meshes of Tibet Bomi Gastrodia elata coarse powder, put it in a volumetric flask and add 0.05mol / L hydrochloric acid, remove the white milky liquid dissolved by hydrochloric acid, repeat several times until the added hydrochloric acid is in a clear state, remove Hydrochloric acid, wash with distilled water after washing with NaOH solution containing a small amount of 1mol / L;

[0039] (2) Add the ethanol-glycerol solution with a volume ratio of 1:1 to suspend the Tibetan Gastrodia elata powder, the mass volume ratio of the Gastrodia elata powder to the ethanol-glycerin solution is 1:5, shake well the Tibetan Gastrodia elata powder and suspend Liquid, use a pipette gun to draw 6 suspensions of different volumes onto the glass slide and heat and ...

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Abstract

The invention relates to a micro-examination flaking method of rhizoma gastrodiae powder and application of the method, and belongs to the technical field of micro-examination. The method includes thefollowing steps of firstly, taking rhizoma gastrodiae coarse powder, adding diluted hydrochloric acid to remove white milky liquid dissolved out by the diluted hydrochloric acid, and washing the rhizoma gastrodiae coarse powder with a NaOH water solution and then distilled water; secondly, adding an ethanol-glycerol solution to obtain a suspension, sucking the suspension onto a glass slide, and heating and volatilizing a solvent to gather the rhizoma gastrodiae coarse powder; thirdly, dropwise adding a chloral hydrate solution to the rhizoma gastrodiae coarse powder to be heated and permeabilized; fourthly, dropwise adding a triacetin water solution, covering and evening out the coarse powder with cover glass, and using a microscope for observation and photography. By means of the method,rhizoma gastrodiae cells can be clearly seen under the microscope, the number of the cells can be easily counted, the linear relationship between the number of the rhizoma gastrodiae powder cells andthe quality is determined, the accuracy is higher, and the fragmentation efficiency of superfine powder is more scientifically calculated.

Description

technical field [0001] The invention relates to a microscopic observation and preparation method of Gastrodia elata powder and an application thereof, belonging to the field of microscopic technology. Background technique [0002] Ultrafine pulverization technology is a new technology developed rapidly in recent years. At present, there are several patents on the broken-walled decoction pieces of Gastrodia elata. Compared with the conventional decoction pieces of Gastrodia elata, the utilization rate of active ingredients in the broken-walled decoction pieces of Gastrodia elata prepared after ultrafine pulverization is higher, which can maximize the medicinal activity of Gastrodia elata; ultrafine pulverization technology It is the key technology in the preparation of Gastrodia elata broken wall decoction pieces, and an important index for evaluating the effect of ultrafine pulverization is the cell wall breaking rate. However, at present, there is no clear calculation meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/2813G01N1/286
Inventor 龙海林蔡鸿飞袁诚许文东张懿李康强潘裕清李海波杨阳蒋兆健
Owner GUANGZHOU HANFANG PHARMA
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