Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of polypeptide sle2018-v003 in diagnostic kit for systemic lupus erythematosus

A technology of SLE2018-V003 and diagnostic kit, which is applied in the field of biomedicine to achieve the effect of assisting early diagnosis of systemic lupus erythematosus, high sensitivity and high specificity

Active Publication Date: 2022-02-25
SHANGHAI JIAOTONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many similar works, but the screening of biomarkers of systemic lupus erythematosus through random peptide library has not been reported. Therefore, we use high-throughput phage display library technology to screen peptides with high discrimination between patients and normal people , in order to obtain more effective early diagnostic markers for systemic lupus erythematosus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of polypeptide sle2018-v003 in diagnostic kit for systemic lupus erythematosus
  • Application of polypeptide sle2018-v003 in diagnostic kit for systemic lupus erythematosus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This embodiment relates to a polypeptide SLE2018-V003, and a diagnostic kit for diagnosing systemic lupus erythematosus comprising the polypeptide. details as follows:

[0051] 1. Prepare the peptide and couple the peptide to BSA

[0052] 1) Polypeptide SLE2018-V003 (amino acid sequence: ISTVYTGLTEKD) is a polypeptide with cysteine ​​at its C-terminus synthesized by Gill Biochemical (Shanghai) Co., Ltd. by HPLC. SMCC (succinimidyl cyclohexane-1-carboxylate) was purchased from Thermo (Cat. No. 22360). Dissolve in DMSO (dimethyl sulfoxide) to a final concentration of 100 mg / mL, and store at -20°C. The shelf life is one month.

[0053] 2) Weigh 10 mg of BSA and add 1 mL of coupling buffer PBS to prepare a 10 mg / mL BSA solution.

[0054] 3) Take 2mg of SMCC (the relationship with BSA is 1:5), add it to the above carrier protein solution, mix well, and react at 25°C for 1h.

[0055] 4) In a 10KD dialysis bag, dialyze overnight.

[0056] 5) Place the dialyzed carrier pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses the application of a polypeptide SLE2018‑V003 in a kit for diagnosing systemic lupus erythematosus; the kit uses the polypeptide SLE2018‑V003 with an amino acid sequence of ISTVYTGLTEKD as an antigen, and connects it to BSA via SMCC to coat a microwell plate. Make a solid-phase antigen, add the serum to be tested into the antigen-coated microwells in turn, and then add the enzyme-labeled reagent containing HRP-labeled anti-Human IgG antibody to form a polypeptide-antibody-enzyme-labeled secondary antibody complex, after washing Add 3,3',5,5'-tetramethylbenzidine (TMB) for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid, and the level of IgG antibody that specifically recognizes the polypeptide in the sample is detected by the depth of the color. The kit provided by the invention can greatly improve the specificity and sensitivity of early diagnosis of systemic lupus erythematosus.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of a polypeptide SLE2018-V003 in a kit (composition) for diagnosing systemic lupus erythematosus. Background technique [0002] Systemic lupus erythematosus (SLE) is an autoimmune-mediated autoimmune disease (autoimmune diseases, ADs) clinically manifested as multi-system damage, often causing irreversible damage to multiple organ systems, seriously affecting the patient's longevity and quality of life. The incidence of systemic lupus erythematosus disease ranges from 0.02% to 0.07%, and the incidence rate of urban population is higher. The age of patients is mainly distributed between 15 and 45 years old, and most of them are female patients. The incidence ratio of male to female is about 1:9 (Lupus 2006.15(5):308–318.). The prevalence, pathological mechanism and biomarkers of systemic lupus erythematosus are three important aspects of systemic lupus eryth...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08G01N33/68G01N33/535G01N33/564
Inventor 陶生策吴凡林祁环李华赖丹昀胡传圣赵小东沈南唐元家郭强丁慧华
Owner SHANGHAI JIAOTONG UNIV