Preparation method of sweet viburnum cutting rooting agent
A technology for rooting agent and coral tree, applied in the field of preparation of rooting agent for coral tree cuttings, can solve the problems of limited rapid effect of rooting agent, inability to exert the physiological effect of rooting agent for a long time, etc. Inhibition of α-glucosidase activity
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Embodiment 1
[0032] Present embodiment relates to a kind of preparation method of coral tree cutting rooting agent, specifically comprises the following steps:
[0033] Enzymolysis: take Cyperus cyperi to remove hair, dry and crush into 80 mesh Cyperus cyperi powder, add 20 parts of distilled water, 0.03 part of cellulase and 0.05 part of protease to each powder of Cyperus cyperi, mix well and heat to 42 ℃, enzymatically hydrolyze for 15 minutes, then lower the temperature to 40℃, add 0.2 parts of stevioside aqueous solution, continue to enzymatically hydrolyze for 15 minutes, and then inactivate the enzyme to obtain the enzymatic solution; use cellulase and protease to enzymolyze Cyperus cyperi powder, first The cellulose on the powder surface is decomposed and degraded, and then the protein is degraded by protease, exposing the polysaccharide of Rhizoma rotifera, and the decrease of the substrate concentration in the middle of the enzymatic hydrolysis reduces the enzymatic hydrolysis effi...
Embodiment 2
[0042] A method for preparing a coral tree cutting and rooting agent, comprising: enzymatic hydrolysis, concentration, and compounding, specifically:
[0043] Enzymolysis: take Cyperus cyperi to remove hair, dry and crush into 200 mesh Cyperus cyperi powder, add 30 parts of distilled water, 0.08 part of cellulase and 0.1 part of protease to each powder of Cyperus cyperi, mix well and heat to 45 ℃, enzymatically hydrolyze for 20 minutes, then lower the temperature to 43℃, add 0.25 parts of stevioside aqueous solution, continue to enzymatically hydrolyze for 20 minutes, and then inactivate the enzyme to obtain the enzymatic solution; use cellulase and protease to enzymolyze Cyperus cyperi powder, first The cellulose on the powder surface is decomposed and degraded, and then the protein is degraded by protease, exposing the polysaccharide of Rhizoma rotifera, and the decrease of the substrate concentration in the middle of the enzymatic hydrolysis reduces the enzymatic hydrolysis ...
Embodiment 3
[0055] The preparation method of coral tree cutting rooting agent comprises the following steps:
[0056] Enzymolysis: take Cyperus cyperi to remove the hair, dry it and crush it into 160 mesh Cyperus cyperi powder, add 25 parts of distilled water, 0.05 part of cellulase and 0.08 part of protease to each powder of Cyperus cyperi, mix well and heat to 44 ℃, enzymatically hydrolyze for 18 minutes, then lower the temperature to 42℃, add 0.24 parts of stevioside aqueous solution, continue to enzymatically hydrolyze for 18 minutes, and then inactivate the enzyme to obtain the enzymatic solution; use cellulase and protease to enzymolyze Cyperus cyperi powder, first The cellulose on the powder surface is decomposed and degraded, and then the protein is degraded by protease, exposing the polysaccharide of Rhizoma rotifera, and the decrease of the substrate concentration in the middle of the enzymatic hydrolysis reduces the enzymatic hydrolysis efficiency of cellulase and protease. At t...
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