Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for adjusting the culture process of microalgae under nitrogen nutrient stress based on feedback control

A regulation method and feedback control technology, which can be used in material excitation analysis, fluorescence/phosphorescence, etc., to solve the problems of inability to accurately control the degree of microalgae stress and the inability to monitor the degree of stress online.

Active Publication Date: 2021-03-30
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a microalgae moderate nitrogen stress regulation technology based on feedback control, in order to improve the controllability of microalgae culture, solve the problem that the stress degree in the current microalgae nitrogen stress culture process cannot be monitored online and cannot realize the degree of microalgae stress The problem of precise control

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for adjusting the culture process of microalgae under nitrogen nutrient stress based on feedback control
  • A method for adjusting the culture process of microalgae under nitrogen nutrient stress based on feedback control
  • A method for adjusting the culture process of microalgae under nitrogen nutrient stress based on feedback control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A 500mL columnar glass bubbler reactor ( figure 2 ) culture of Nannochloropsis mesophyllum as an example, the reaction conditions are culture temperature 25±2℃, light intensity 140μmol m –2 the s –1 , air CO 2 The ventilation volume is 100mL min –1 , only when the light passes into the CO 2 , CO 2 The amount of inflow is 2% of the air inflow, the light-dark ratio is 14h:10h, and the light time is 8:00-20:00 every day. Seawater culture, using improved F / 2 nutrient salts, the final concentration of each nutrient salt in the mother liquor is (per liter): 75.00g NaNO 3 , 5.00g NaH 2 PO 4 , 3.15g FeCl 3 ·6H 2 O, 4.36 g Na 2 EDTA, 9.80 mg CuSO 4 ·5H 2 O, 6.30 mg Na 2 MoO 4 2H 2 O, 22.00mgZnSO 4 ·7H 2 O, 10.00 mg CoCl 2 ·6H 2 O, 0.18g MnCl 2 4H 2 O, 1.00mg vitamin B12, 0.20g vitamin B1, 1.10mg biotin. The nitrogen nutrient concentration in the system is expressed by the final concentration of nitrogen element.

[0040] In this example, microalgae cell c...

Embodiment 2

[0046] In the present embodiment, the microalgae cell culture of 4 kinds of culture conditions is carried out, except culture condition 4, other 3 kinds of cultures are the same as embodiment 1. Culture condition 4 adopts semi-continuous culture, that is, when the degree of cell stress reaches the set value, it is diluted according to the set dilution rate, and nutrients are added (the specific addition method is the same as that of culture condition 4 in Example 1), and a new culture is started. cycle. Among them, the dilution rate refers to the ratio of the volume of fresh medium added during re-dilution to the total culture volume during semi-continuous culture. Specifically, in the culture condition 4 of this example, the NSI is controlled at 0.73 and the intracellular nitrogen content is 3.73%wt. At this time, the online characterization parameter ΔF / F of the current nitrogen stress degree m ’ with a value of 0.503. Figure 5 is based on ΔF / F m’ Feedback control result...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for adjusting the culture process of microalgae under nitrogen nutrition stress based on feedback control. The present invention defines the nitrogen stress index (Nitrogen Stress Index, NSI) which is used to quantify the degree of nitrogen stress suffered by microalgae cells, and provides a control index for accurately controlling the degree of nitrogen stress in the microalgae culture process, and by establishing a parameter based on chlorophyll fluorescence The in-situ online characterization method of the nitrogen stress degree realizes the feedback control of the nitrogen stress degree of microalgal cells based on the guidance of chlorophyll fluorescence parameters, and ensures that the microalgal cells are under appropriate stress.

Description

technical field [0001] The invention relates to a microalgae moderate nutritional stress regulation technology based on feedback control, specifically using microalgae chlorophyll fluorescence parameters (F v / F m or ΔF / F m ’) To characterize the nitrogen stress degree of microalgae cells in real time, and feedback to guide the addition of nutrient elements during the cultivation process, so as to control the microalgae in an appropriate stress state. Background technique [0002] Due to their high photosynthetic efficiency and the advantages of not competing with agriculture for land, microalgae have received great attention in the strategic research of renewable biomass energy at home and abroad. Under nutrient-rich conditions, the cells of microalgae can fix CO 2 And the absorbed nutrients are mainly used for the growth and reproduction of the cells themselves. When cells are under stress (such as nutrient stress, environmental stress, etc.), the energy and CO fixed b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 薛松刘娇曹旭鹏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI