Single-chain antibody targeting CD22, chimeric antigen receptor T cell, preparation method and application thereof

A chimeric antigen receptor and single-chain antibody technology, applied in the field of medical biology, can solve the problems of failure to kill tumor cells, CART-CD19 can no longer exert anti-tumor effect, and achieve high infection efficiency and transcription efficiency, long-lasting The effect of maintaining capacity in vivo

Inactive Publication Date: 2019-06-04
SHENZHEN BINDEBIOTECH CO LTD
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since tumors of the same type often express different immune phenotypes, for example, when tumor cells escape CD19-negative, that is, there are tumor cells that do not express CD19, the CD19 target will fail, and CART-CD19 will no longer be able to function. Anti-tumor effect, therefore, it is necessary to provide CAR-T cells that can target other broadly expressed targets of malignancies
In addition, the in vivo maintenance ability (persistence) of CAR-T cells is the guarantee of the long-term efficacy of CAR-T, but in fact, CAR-T completely disappears after a few days to a few weeks after reinfusion, and fails to exert a long-lasting and lasting effect. ability to kill tumor cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Single-chain antibody targeting CD22, chimeric antigen receptor T cell, preparation method and application thereof
  • Single-chain antibody targeting CD22, chimeric antigen receptor T cell, preparation method and application thereof
  • Single-chain antibody targeting CD22, chimeric antigen receptor T cell, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A method for preparing chimeric antigen receptor T cells targeting CD22, comprising the following steps:

[0078] (1) Prepare the gene sequence of chimeric antigen receptor CAR-CD22 targeting CD22

[0079]The coding genes of the signal peptide, CD22-targeting single-chain antibody, CD8α hinge region, CD8 transmembrane region, 4-1BB signal region and CD3ζ signal region were prepared respectively, and the coding gene sequence of the signal peptide was shown in SEQ ID NO: 15. The coding gene sequence of the single-chain antibody targeting CD22 is shown in SEQ ID NO: 2, the coding gene sequence of the CD8α hinge region is shown in SEQ ID NO: 7, and the coding gene sequence of the CD8 transmembrane region The sequence is shown in SEQ ID NO: 9, the coding gene of the 4-1BB signal region is shown in SEQ ID NO: 11, and the coding gene of the CD3ζ signal region is shown in SEQ ID NO: 13.

[0080] The genes encoding the above signal peptide, CD22-targeting single-chain antibody,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides a single-chain antibody targeting CD22, the single-chain antibody targeting CD22 comprises an amino acid sequence shown in SEQ ID NO: 1, and the single-chain antibody targeting CD22 is a humanized single-chain antibody. The present invention also provides a chimeric antigen receptor T cell comprising the single-chain antibody targeting CD22, wherein the chimeric antigen receptor targeting CD22 can specifically target CD22, especially can be targeted to tumor cells that do not express CD19, the expansion of T cells in patients is promoted, and the tumor cells canbe efficiently and specifically killed. In addition, the chimeric antigen receptor T cell targeting CD22 produced by humanized single-chain antibody has better cell viability and lethality. The invention also provides a preparation method and an application of the chimeric antigen receptor T cell targeting CD22.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a single-chain antibody targeting CD22, a chimeric antigen receptor T cell, and a preparation method and application thereof. Background technique [0002] CAR-T (Chimeric Antigen Receptor T Cell) technology is a new type of cell therapy, which is to reinfuse CAR-modified T cells into the human body, activate the autoimmune system, and kill tumor cells. The treatment of Hodgkin's lymphoma has a significant effect and is considered to be one of the most effective treatments for malignant tumors. [0003] At present, CAR-T technology has achieved remarkable results in CD19-positive blood tumors (such as B-cell lymphoma, acute and chronic B-lymphoblastic leukemia). However, since tumors of the same type often express different immune phenotypes, for example, when tumor cells escape CD19-negative, that is, there are tumor cells that do not express CD19, the CD19 target will fail, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C12N15/13C07K19/00C12N15/62C12N15/867C12N5/10A61P35/00
Inventor 曾滢张长风吴咏东
Owner SHENZHEN BINDEBIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap