Method for screening heat-resistant chrysanthemum

Abstract

The invention discloses a method for screening heat-resistant chrysanthemum, which comprises the following steps of: carrying out a high-temperature stress treatment on the chrysanthemum at a seedlingstage, and observing and statistically detecting severity of high-temperature injury symptoms, a survival rate of seedlings, an oxidative injury degree and an expression level of heat shock related genes embodied by various varieties; carrying out the high-temperature stress treatment on florescence chrysanthemum, and observing and statistically detecting the severity of the high-temperature injury symptoms embodied by the various varieties, whether flowering or not and a level of related physiological indexes; cultivating chrysanthemum seedlings in tropical regions, carrying out physiological and biochemical index determination, and observing and counting a flowering rate of the various varieties and a length of the florescence; and screening heat-resistant chrysanthemum varieties by integrating results of all indexes in the three steps. The method for screening the heat-resistant chrysanthemum combines high-temperature stress treatment and field cultivation tests, which is an effective way for efficiently and comprehensively obtaining heat-resistant varieties (strain), and is a good research method for introducing cultivation and breeding the heat-resistant chrysanthemum varieties in a high-temperature area of the south.

Description

technical field [0001] The invention relates to a method for screening plant varieties, in particular to a method for screening heat-resistant chrysanthemums. Background technique [0002] Chrysanthemum (Chrysanthemum morifolium) is a perennial herbaceous perennial flower of the genus Chrysanthemum in the family Asteraceae. It is one of the top ten traditional famous flowers in China, one of the top four cut flowers and top ten potted flowers in the world. It has extremely high ornamental and application value, and plays a very important role in flower production. status. [0003] Chrysanthemums like mild and cool climates, and the optimum temperature for growth and development is between 15 and 25°C. High temperatures above 25°C are not conducive to the growth of chrysanthemums. It has been reported that chrysanthemum grows slowly when the temperature is above 32°C, and can withstand high temperatures of 40°C in summer, but basically stops growing. The high temperature in...

AI Technical Summary

Problems solved by technology

However, it takes too long to obtain transgenic plants, and the difficulty of transgenic is related to the variety. At present, the transgenic technology of chrysanthemums is not mature enough, and it is still difficult to successfully transfer genes into chrysanthemum varieties.

[0006] Most of the previous studies were to observe the heat resistance comparison of experimental chrysanthemum varieties under simulated high temperature. However, on the one hand, the direct identification of artificial simulation will be limited by equipment factors, and it cannot completely simulate all natural conditions and the amount of experimental materials is limited.

On the other hand, the field test results are easily affected by the location, and the reliability of the local test results is small

[0007] So far, no relevant reports have been found on the introduction of chrysanthemums and the selection of heat-resistant chrysanthemum varieties in tropical regions

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