Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

PIK3CA gene g.1792224821G>A mutation and application thereof to auxiliary diagnosis of breast cancer

A gene and mutant technology, applied in the fields of medicine and biology, can solve the problems of unsatisfactory overall survival rate, lack of treatment methods for patients with middle and advanced stages, and lack of therapeutic targets for drugs, so as to achieve the effect of convenient and easy diagnosis.

Active Publication Date: 2019-06-21
WUXI NO 5 PEOPLES HOSPITAL
View PDF10 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the improvement of early detection and diagnosis technology, the progress of surgery and comprehensive adjuvant therapy have significantly improved the prognosis of patients, the overall survival rate is not ideal
For specific molecular subtypes, due to primary or secondary resistance to drugs, or even the lack of specific therapeutic targets, some patients with advanced disease lack effective treatments

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • PIK3CA gene g.1792224821G>A mutation and application thereof to auxiliary diagnosis of breast cancer
  • PIK3CA gene g.1792224821G>A mutation and application thereof to auxiliary diagnosis of breast cancer
  • PIK3CA gene g.1792224821G>A mutation and application thereof to auxiliary diagnosis of breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0026] Its specific implementation is as follows:

[0027] For the preparation of the DNA template, the purchased kit was used to extract whole blood genomic DNA, and the specific steps were as follows:

[0028] (1) Take a sterile 2.0mL centrifuge tube and add 1mL of cell lysate;

[0029] (2) Gently shake the whole blood sample anticoagulated with EDTA until it is thoroughly mixed; then draw 500 μL of blood sample into the above-mentioned centrifuge tube containing the cell lysate, and gently pour the centrifuge tube 5 to 6 times to mix;

[0030] (3) Incubate at room temperature for 10 minutes (during this period, invert the centrifuge tube 2 to 3 times to mix);

[0031] (4) 12000rpm room temperature centrifugation for 5 minutes;

[0032] (5) Use a pipette to slowly remove the supernatant as much as possible, and be careful not to suck out the white substance at the junction of the two phases;

[0033] (6) Use a vortex shaker (Votex) to mix vigorously until the leukocytes a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a PIK3CA gene g.1792224821G>A mutation and application thereof to auxiliary diagnosis of breast cancer and belongs to the field of medical and biological technology. Compared in sequence with PIK3CA normal genes, the PIK3CA gene g.1792224821G>A mutation has a g.1792224821G>A site mutation. The invention also provides a breast cancer pathogenesis new mutation site, a specific primer for detecting the breast cancer pathogenesis new mutation site and a kit containing the specific primer. The PIK3CA gene g.1792224821G>A mutation can be applied to early diagnosis of breast cancer.

Description

technical field [0001] The invention belongs to the field of medicine and biotechnology, and specifically relates to a PIK3CA gene g.1792224821G>A site mutation in breast cancer and its application in auxiliary preparation of breast cancer diagnostic reagents. Background technique [0002] Breast cancer is the most common malignant tumor faced by women all over the world, and it is also an important cause of female death. Since the 1990s, the growth rate of breast cancer incidence in China is more than twice that of the world. The number of new breast cancer cases and deaths in China account for 12.2% and 9.6% of the world's annual breast cancer incidence respectively. At present, breast cancer is the cancer with the highest incidence rate in Chinese women, and the sixth cause of cancer death, with a significant trend of younger age. Although the improvement of early detection and diagnostic techniques, as well as the advancement of surgery and comprehensive adjuvant the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/54C12N15/11C12Q1/6886
Inventor 王学东张兵周道平王玥苹吴怀国顾娟
Owner WUXI NO 5 PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com