PIK3CA gene g.1792224821G>A mutation and application thereof to auxiliary diagnosis of breast cancer

A gene and mutant technology, applied in the fields of medicine and biology, can solve the problems of unsatisfactory overall survival rate, lack of treatment methods for patients with middle and advanced stages, and lack of therapeutic targets for drugs, so as to achieve the effect of convenient and easy diagnosis.

Active Publication Date: 2019-06-21
WUXI NO 5 PEOPLES HOSPITAL
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  • Abstract
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Problems solved by technology

Although the improvement of early detection and diagnosis technology, the progress of surgery and comprehensive adjuvant therapy have significantly improved the prognosis of patients, the overall survival ra

Method used

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  • PIK3CA gene g.1792224821G>A mutation and application thereof to auxiliary diagnosis of breast cancer
  • PIK3CA gene g.1792224821G>A mutation and application thereof to auxiliary diagnosis of breast cancer
  • PIK3CA gene g.1792224821G>A mutation and application thereof to auxiliary diagnosis of breast cancer

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Experimental program
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Embodiment approach

[0026] Its specific implementation is as follows:

[0027] For the preparation of the DNA template, the purchased kit was used to extract whole blood genomic DNA, and the specific steps were as follows:

[0028] (1) Take a sterile 2.0mL centrifuge tube and add 1mL of cell lysate;

[0029] (2) Gently shake the whole blood sample anticoagulated with EDTA until it is thoroughly mixed; then draw 500 μL of blood sample into the above-mentioned centrifuge tube containing the cell lysate, and gently pour the centrifuge tube 5 to 6 times to mix;

[0030] (3) Incubate at room temperature for 10 minutes (during this period, invert the centrifuge tube 2 to 3 times to mix);

[0031] (4) 12000rpm room temperature centrifugation for 5 minutes;

[0032] (5) Use a pipette to slowly remove the supernatant as much as possible, and be careful not to suck out the white substance at the junction of the two phases;

[0033] (6) Use a vortex shaker (Votex) to mix vigorously until the leukocytes a...

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Abstract

The invention discloses a PIK3CA gene g.1792224821G>A mutation and application thereof to auxiliary diagnosis of breast cancer and belongs to the field of medical and biological technology. Compared in sequence with PIK3CA normal genes, the PIK3CA gene g.1792224821G>A mutation has a g.1792224821G>A site mutation. The invention also provides a breast cancer pathogenesis new mutation site, a specific primer for detecting the breast cancer pathogenesis new mutation site and a kit containing the specific primer. The PIK3CA gene g.1792224821G>A mutation can be applied to early diagnosis of breast cancer.

Description

technical field [0001] The invention belongs to the field of medicine and biotechnology, and specifically relates to a PIK3CA gene g.1792224821G>A site mutation in breast cancer and its application in auxiliary preparation of breast cancer diagnostic reagents. Background technique [0002] Breast cancer is the most common malignant tumor faced by women all over the world, and it is also an important cause of female death. Since the 1990s, the growth rate of breast cancer incidence in China is more than twice that of the world. The number of new breast cancer cases and deaths in China account for 12.2% and 9.6% of the world's annual breast cancer incidence respectively. At present, breast cancer is the cancer with the highest incidence rate in Chinese women, and the sixth cause of cancer death, with a significant trend of younger age. Although the improvement of early detection and diagnostic techniques, as well as the advancement of surgery and comprehensive adjuvant the...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N15/11C12Q1/6886
Inventor 王学东张兵周道平王玥苹吴怀国顾娟
Owner WUXI NO 5 PEOPLES HOSPITAL
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