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Method for preparing sedum sarmentosum extract and use thereof for preparing medicine for relaxing airway smooth muscle

A technology of weeping grass and extracts, applied in the field of medicine, can solve the problems of no research reports on the intervention effect, and achieve the effect of alleviating airway obstruction

Inactive Publication Date: 2019-07-02
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the process of deepening the research, the applicant was prompted by the research reports on some components of Shuipencao, and realized that Shuipencao may have a certain effect on the relaxation of tracheal smooth muscle, but for its role in respiratory diseases (asthma, etc.) and The intervention effect on airway hyperresponsiveness has not been reported

Method used

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  • Method for preparing sedum sarmentosum extract and use thereof for preparing medicine for relaxing airway smooth muscle
  • Method for preparing sedum sarmentosum extract and use thereof for preparing medicine for relaxing airway smooth muscle
  • Method for preparing sedum sarmentosum extract and use thereof for preparing medicine for relaxing airway smooth muscle

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Experimental program
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Effect test

Embodiment 1

[0030] The preparation of embodiment 1 weeping pot grass extract

[0031] Preparation methods such as figure 1 As shown, the specific preparation results of the Herba chinensis extract are as follows:

[0032] 1) Take the whole herb of C. chinensis, crush it, add 70% ethanol according to the ratio of 1:7 and soak it at room temperature for 72 hours, extract by reflux to obtain the extract, and evaporate the extract to dryness under reduced pressure to obtain the crude extract of C. Ethanol extract of bonsai;

[0033] 2) adding petroleum ether to the ethanol extract of C. vulgaris to obtain a petroleum ether phase and a first water phase, and concentrating the petroleum ether phase under reduced pressure to obtain C. vulgaris petroleum ether extract;

[0034] 3) adding ethyl acetate to the above-mentioned first water phase and extracting and layering to obtain an ethyl acetate phase and a second water phase, and the ethyl acetate phase is concentrated under reduced pressure t...

Embodiment 2

[0036] Example 2 The pre-contraction of the airway smooth muscle induced by the relaxing acetylcholine of the weeping grass extract

[0037] 2.1. Isolation of isolated mouse trachea

[0038] Purchase 6-8 week old male adult BALB / c mice and place them in an SPF animal room for 3 days. After removal, mice were quickly sacrificed using cervical dislocation and fixed on a dissection board. Spray 75% alcohol from the abdomen to the head, and cut the epidermis of the mouse with scissors, then stir up the flat scissors in a slightly right direction to the upper respiratory tract, remove the thymus and excess connective tissue, and then cut the lower connection from the dorsal aorta Keep the normal length of the mouse trachea and cut it close to the inner wall of the larynx. After that, transect the trachea and carefully remove the heart, and then quickly place it in the pre-cooled high calcium solution on ice. Separate the main trachea under a dissecting microscope and use a hook t...

Embodiment 3

[0045] Example 3 Ethyl acetate extract of weeping pot grass by down-regulating intracellular Ca 2+ to relax tracheal smooth muscle.

[0046] Detection of intracellular Ca by Fura-2AM staining of tracheal smooth muscle cells 2+ level, and then add 100 μM ACH to elicit intracellular Ca 2+ 3.98mg / mL Ethyl acetate extract + 100μM ACH was further added to verify that the effect of relaxing tracheal smooth muscle is related to intracellular Ca 2+ concentrations are closely related.

[0047] Experimental results such as image 3 As shown, the experimental results show that: 100μM ACH can significantly trigger Ca in smooth muscle cells 2+ increase (p2+ The rise was completely suppressed (p<0.001).

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Abstract

The invention provides a method for preparing sedum sarmentosum extract and use thereof for preparing medicine for relaxing airway smooth muscle. The sedum sarmentosum extract includes at least one ofan ethanol extract of sedum sarmentosum, a petroleum ether extract of sedum sarmentosum, an ethyl acetate extract of sedum sarmentosum, a n-butanol extract of sedum sarmentosum, and a residual water-phase concentrate after extraction and separation. In the present application, through the detection of isolated tracheal ring muscle tension in experimental mice and Ca<2+> detection in airway smoothmuscle cells, the sedum sarmentosum extract is a highly effective drug for relaxing airway smooth muscle, and the relaxation is caused by blocking LVDCCs and activating calcium channels such as BKCa.The above research results can confirm that sedum sarmentosum extract has the ability to relieve airway obstruction of asthma patients and can be used in the preparation of drugs for relaxing airwaysmooth muscle.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of the extract of sycamore in the preparation of medicine for relaxing airway smooth muscle. Background technique [0002] Sustained contraction of airway smooth muscle and increased calcium sensitivity are one of the main symptoms of asthma and chronic obstructive pulmonary disease. Among the above drugs for the treatment of respiratory diseases, bronchodilator drugs such as glucocorticoids are common clinical drugs. In view of the long-term use of clinical hormone drugs will produce drug resistance and side effects and other disadvantages, the development of safer and more reliable bronchodilators has become the main way to solve the above problems. As a big country in the use and production of traditional Chinese medicine, our country has abundant medicinal plant resources, which are a huge resource treasure house for new drug screening. It has ...

Claims

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Application Information

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IPC IPC(8): A61K36/41A61P11/06A61P11/00
CPCA61K36/41A61K2236/333A61K2236/55A61P11/00A61P11/06
Inventor 彭勇波王齐刘庆华
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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