A kind of rice double haploid breeding method based on haploid induction line

Abstract

The invention provides a rice doubled haploid breeding method based on a haploid inducer and belongs to the technical field of agriculture. According to the method herein, a rice three-line maintainermaterial is hybridized with a rice material having a dominant red rice gene, a red rice maintainer carrying the dominant red rice gene are attained by screening, the red rice maintainer is subjectedto chemical directional induction, a mutant with a centromere mutant gene function is attained by screening, and the mutant is hybridized with a rice three-line sterile line to obtain a maintainer andsterile line carrying the red rice gene and centromere mutant gene; a hybrid F1 prepared according to breeding targets is hybridized with a red rice haploid induced sterile line before colorless-coathybrid seeds are retained; the colorless hybrid seeds are treated with colchicine to obtain a diploid material, namely, a novel line is formed. The rice doubled haploid breeding method based on the haploid inducer allows a homozygous gene to be attained quickly, helps overcome the defect segregative generations in traditional breeding are long, and is applicable to rice breeding.

Description

technical field [0001] The invention relates to a rice double haploid breeding method based on a haploid induction system, which belongs to the field of agricultural technology. Background technique [0002] Haploid breeding technology is to obtain purified double haploid (DH plants) by doubling the chromosome group of natural or artificially cultivated haploid plants. It is more and more popular among breeders for its fast, accurate and efficient characteristics At present, more than 250 crop species in the world have applied double haploid breeding, and more than 300 double haploid pure lines from cultivated species have been bred in 12 species. The core of traditional breeding technology is to fix favorable genes and make them homozygous through continuous selection. This process usually requires multiple generations of self-crossing or backcrossing. Lines need more than 6 generations, and the use of double haploid breeding method generally only needs 2 generations, whic...

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Problems solved by technology

There are obvious deficiencies in distant hybridization, anther culture, parthenogenesis and other methods: distant hybridization has extremely low induction rate; anther culture has low anther culture efficiency, genotype disorder, callus induction rate and green shoot differentiation rate Low; the frequency of parthenogenesis is extremely low, the effect is unstable, the difference between genotypes is large, and the number of seeds obtained is small, etc.

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