Human gamete rapid freezing and thawing visualization environment chamber maintained at normal pressure air atmosphere

Abstract

A human gamete rapid freeze-thaw visualization environmental chamber that maintains an atmospheric air atmosphere, mainly composed of the main body of the environmental chamber, a high thermal conductivity bottom plate, an upper flange cover, an O-ring, a pre-tightening screw, a capillary, a sterilizing dryer, and a thermocouple and an electric heating film. The main body of the environmental chamber is a flat hollow cylindrical structure, which forms a closed cavity together with the high thermal conductivity bottom plate and the upper flange cover. The center of the upper flange cover plate is embedded with high-transparency quartz glass, which is buckled on the top surface of the main body of the environmental chamber. The side of the main body of the environmental chamber is provided with a capillary interface, a thermocouple penetration interface, an electric heating film penetration interface, and a connection pole. The invention can realize rapid freezing and thawing of human gamete samples under constant normal pressure conditions, and has full-process visualization conditions and real-time temperature measurement capabilities, and is suitable for observing the transient vitrification or crystallization freezing and thawing of gametes in the process of low temperature cooling or rewarming process.

Description

technical field [0001] The invention relates to a human gamete rapid freeze-thaw visualization environment chamber that maintains a normal-pressure air atmosphere, and in particular to a gamete sample that can be repeatedly loaded, which can make the sample contact with a low-temperature cold surface at intervals but can quickly cool down, and can always ensure a certain temperature during the period. Oxygen atmospheric pressure environment and internal high-transparency visible device. Background technique [0002] Vitrification technology has gradually become one of the important application technologies in assisted reproductive technology. The principle is that biological samples are directly put into liquid nitrogen or low-temperature steam together with cryoprotectants to obtain a sufficient cooling rate so that no cells are formed inside or outside the cells. Ice crystals to avoid physical damage to cells. The vitrification cryopreservation method has the advantages o...

AI Technical Summary

Problems solved by technology

In this patent, the gamete carrier is placed in a liquid nitrogen fumigation box without an observation window, let alone the conditions for visually observing the gamete freeze-thaw phenomenon

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