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Human gamete rapid freezing and thawing visualization environment chamber maintained at normal pressure air atmosphere

An environmental chamber and air technology, applied in the preservation, application, animal husbandry, etc. of human or animal body, can solve the problem of no visual observation of gamete freezing and thawing phenomenon, achieve rapid and controlled rewarming, avoid bacterial virus or Impurity infection, the effect of facilitating imaging

Active Publication Date: 2021-02-02
上海艾尔温生命科技有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

In this patent, the gamete carrier is placed in a liquid nitrogen fumigation box without an observation window, let alone the conditions for visually observing the gamete freeze-thaw phenomenon

Method used

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  • Human gamete rapid freezing and thawing visualization environment chamber maintained at normal pressure air atmosphere
  • Human gamete rapid freezing and thawing visualization environment chamber maintained at normal pressure air atmosphere
  • Human gamete rapid freezing and thawing visualization environment chamber maintained at normal pressure air atmosphere

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Embodiment Construction

[0024] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0025] see Figure 1 to Figure 3 , as can be seen from the figure, the human gamete rapid freeze-thaw visualization environment chamber for maintaining normal pressure air atmosphere in the present invention includes an environment chamber main body 1, a high thermal conductivity bottom plate 2, an upper flange cover plate 3, an O-ring 4, a pre-tightening screw 5, Capillary 6, sterilizing dryer 7, thermocouple 8 and electric heating membrane 9, the main body 1 of the environmental chamber is a flat hollow cylindrical structure, and together with the high thermal conductivity bottom plate 2 and the upper flange cover 3 form a closed Cavity; the outer surface of the environmental chamber main body 1 is provided with a capillary interface 10, a thermocouple penetration interface 11, an electric heating film penetration interface 12 and a connect...

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Abstract

A human gamete rapid freeze-thaw visualization environmental chamber that maintains an atmospheric air atmosphere, mainly composed of the main body of the environmental chamber, a high thermal conductivity bottom plate, an upper flange cover, an O-ring, a pre-tightening screw, a capillary, a sterilizing dryer, and a thermocouple and an electric heating film. The main body of the environmental chamber is a flat hollow cylindrical structure, which forms a closed cavity together with the high thermal conductivity bottom plate and the upper flange cover. The center of the upper flange cover plate is embedded with high-transparency quartz glass, which is buckled on the top surface of the main body of the environmental chamber. The side of the main body of the environmental chamber is provided with a capillary interface, a thermocouple penetration interface, an electric heating film penetration interface, and a connection pole. The invention can realize rapid freezing and thawing of human gamete samples under constant normal pressure conditions, and has full-process visualization conditions and real-time temperature measurement capabilities, and is suitable for observing the transient vitrification or crystallization freezing and thawing of gametes in the process of low temperature cooling or rewarming process.

Description

technical field [0001] The invention relates to a human gamete rapid freeze-thaw visualization environment chamber that maintains a normal-pressure air atmosphere, and in particular to a gamete sample that can be repeatedly loaded, which can make the sample contact with a low-temperature cold surface at intervals but can quickly cool down, and can always ensure a certain temperature during the period. Oxygen atmospheric pressure environment and internal high-transparency visible device. Background technique [0002] Vitrification technology has gradually become one of the important application technologies in assisted reproductive technology. The principle is that biological samples are directly put into liquid nitrogen or low-temperature steam together with cryoprotectants to obtain a sufficient cooling rate so that no cells are formed inside or outside the cells. Ice crystals to avoid physical damage to cells. The vitrification cryopreservation method has the advantages o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0257
Inventor 黄永华吴栋梁张宏彬李铮姚晨成李朋
Owner 上海艾尔温生命科技有限公司
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