Natural composition capable of inhibiting alpha-glucosidase activity and used for regulation of blood glucose and saccharides absorption

A technology of glucosidase and composition, which is applied in the direction of drug combination, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., which can solve the problems of few and limited traditional Chinese medicine compounds

Active Publication Date: 2019-07-12
SYI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many literatures on the regulation of blood sugar by traditional Chinese medicine, most of them are only discussed on a single Chinese medicine or its extract, and there are quite few studies on compound Chinese medicine. Even most of the research on the regulation of blood sugar by Chinese medicine compound is limited to traditional formula

Method used

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  • Natural composition capable of inhibiting alpha-glucosidase activity and used for regulation of blood glucose and saccharides absorption
  • Natural composition capable of inhibiting alpha-glucosidase activity and used for regulation of blood glucose and saccharides absorption
  • Natural composition capable of inhibiting alpha-glucosidase activity and used for regulation of blood glucose and saccharides absorption

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] Preparation of the mixed solution: take Taiwanese cinnamon (Cinnamomum osmophloeum) and hawthorn (Crataegus pinnatifida), wash with deionized water, and extract by reflux for 60 minutes; filter the extract and freeze-dry it into powder; Redissolve in ionic water to a concentration of 100mg / mL, shake until completely dissolved, and then follow the composition of the mixed solution shown in Table 1, for example, the volume of the natural water extract solution of MIX1 soil cinnamon accounts for 40%, and the natural water extract solution of hawthorn The volume accounts for 60%, and the different volume percentages of the solutions are used to form the natural water extraction mixture code MIX-1-5, and the activities of inhibiting α-glucosidase and α-amylase are evaluated. In the present invention, the soil cinnamon refers to the leaf of Taiwan soil cinnamon, and the hawthorn refers to the fruit of hawthorn.

[0037] The composition of table 1 mixture (Composition of mixtu...

Embodiment 1

[0055] Example 1: Evaluation of the efficacy of natural water extracts in inhibiting α-glucosidase activity

[0056] figure 1 The results showed that different natural water extracts and Acarbose were sequentially diluted (0.01-2.56mg / ml) to measure their inhibitory ability to α-glucosidase, and the inhibitory effect of different natural water extracts on α-glucosidase was dose-dependent. Correlation. And different natural water extracts (earth cinnamon C.osmophloeum, hawthorn C.pinnatifida, lotus leaf F.nelumbinis and roselle H.sabdariffa) at a concentration of 0.32mg / ml, the inhibition rate of α-glucosidase activity, Respectively 93.86, 98.74, 89.72 and 66.36%, better than the 45.08% enzyme activity inhibition rate of Acarbose at the same concentration. But other natural water extracts, such as white kidney bean (P.vulgaris), mountain bitter melon (M.charantia), tangerine peel (C.reticulate), have poor inhibitory effect on α-glucosidase activity. Table 2 is the calculatio...

Embodiment 2

[0057] Example 2: Evaluation of the efficacy of natural water extracts in inhibiting α-amylase activity

[0058] The concentration of different natural water extracts is 0.4-25.6mg / ml and the concentration of Acarbose is 0.01-1.28mg / ml, respectively, and its inhibitory ability to α-amylase is measured; figure 2 The results indicated that the inhibitory effect of different natural water extracts on α-amylase was dose-dependent. And observe different natural water extracts respectively, such as hawthorn, mountain bitter melon and tangerine peel, under the treatment of concentration 6.4mg / ml, the inhibitory rate to α-amylase activity reaches 22.51, 18.83 and 9.92% respectively ( figure 2 A); while Acarbose is 34.43% ( figure 2 B).

[0059] Table 2 is the calculation of the half-inhibitory concentration (IC50) of hawthorn (C.pinnatifida), lotus leaf (F.nelumbinis), roselle (H.sabdariffa) and Acarbose on α-amylase activity, which are 8.67±0.29, 25.23± 1.92, 9.91±0.51 and 0.06...

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Abstract

A natural composition comprises cinnamomum osmophloeum and hawthorn; the cinnamomum osmophloeum refers to a water extract of cinnamomum osmophloeum leaves, and the hawthorn refers to a water extract of hawthorn fruits, wherein the weight ratio of the cinnamomum osmophloeum to the hawthorn is 1:(0.05-2), the better ratio is 1:(0.5-1.5), and the optimum ratio is 1:(0.9-1.1). The pharmaceutical composition has the effect of inhibiting alpha-glucosidase. The natural composition can further inhibit alpha-amylase to regulate blood glucose and saccharides absorption.

Description

technical field [0001] The present invention relates to a natural composition, especially a natural composition inhibiting α-glucosidase (α-glucosidase), which is used for regulating blood sugar and sugar absorption. Background technique [0002] Diabetes mellitus (diabetes mellitus) is a chronic hyperglycemic metabolic disease, and it is also recognized as one of the most serious chronic diseases in the world. The main cause is insufficient insulin secretion or the inability of the organism's tissues to effectively utilize glucose, resulting in excessive glucose levels in the blood. Insulin is known to be secreted by β cells in the pancreas, which has the effect of regulating blood sugar and can stimulate fat and muscle cells to transport glucose. When the organism has insufficient insulin secretion or poor sensitivity to insulin due to obesity, aging and other factors, the blood sugar concentration will increase, resulting in diabetes. Diabetic patients may experience sym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/734A61P3/10
CPCA61K36/54A61K36/734A61K2236/331A61K2300/00
Inventor 连秀屘陈家章张筱君黄朝禄许瑞婷
Owner SYI BIOTECH CO LTD
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