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The Application of 20-Hydroxyecdysone in Promoting the Development of Blastospores of Cordyceps sinensis to Form Mycelium

A technology of Cordyceps sinensis fungus and ecdysone, which is applied to microorganism-based methods, fungi, microorganisms, etc., can solve the problems of long time for development and formation of mycelium, long period of artificial cultivation of Cordyceps sinensis, etc., and achieve the effect of improving efficiency.

Active Publication Date: 2021-03-12
INNER MONGOLIA FURUI MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above problems, the purpose of the present invention is to provide the application of 20-hydroxyecdysone in promoting the development and formation of mycelia of Cordyceps fungus blastospores, thereby solving the long time for the development and formation of Cordyceps fungi blastospores and the long period of artificial culture of Cordyceps sinensis

Method used

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  • The Application of 20-Hydroxyecdysone in Promoting the Development of Blastospores of Cordyceps sinensis to Form Mycelium
  • The Application of 20-Hydroxyecdysone in Promoting the Development of Blastospores of Cordyceps sinensis to Form Mycelium

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Preparation of Cordyceps sinensis liquid medium: 30g boiled potato filtrate (weigh 30g peeled potatoes, add appropriate amount of water to boil for 20min, filter, take the filtrate, which is 30g boiled potato filtrate), 1.5g peptone, 3g Maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a 500mL Erlenmeyer flask, and dilute to 150mL with water. After autoclaving, place in a culture room at 12±2°C to pre-cool, add 1mL per 150mL of liquid medium with a concentration of 1.5×10 7 Blastospores / mL were used to inoculate the mother liquor so that the final concentration of blastospores was 10 5 each / mL, and add 20-hydroxyecdysone (without adding 20-hydroxyecdysone as a control) to make the final concentration 2mM, place it on a shaker with a rotation speed of 100 rpm, at 12±2°C, in the dark Cultivate under conditions, cultivate the 2nd day, take out 1mL culture bacterium liquid, microscopic examination finds that blastospore be...

Embodiment 2

[0026] Preparation of Cordyceps sinensis liquid medium: 30g boiled potato filtrate (weigh 30g peeled potatoes, add appropriate amount of water to boil for 20min, filter, take the filtrate, which is 30g boiled potato filtrate), 1.5g peptone, 3g Maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a 500mL Erlenmeyer flask, and dilute to 150mL with water. After autoclaving, place in a culture room at 12±2°C to pre-cool, add 1mL per 150mL of liquid medium with a concentration of 1.5×10 9 Each / mL blastospore was inoculated into the mother liquor so that the final concentration of blastospore was 10 7 each / mL, and add 20-hydroxyecdysone (without adding 20-hydroxyecdysone as a control) to make the final concentration 2mM, place it on a shaker with a rotation speed of 100 rpm, at 12±2°C, in the dark On the second day of cultivation, take out 1mL of the culture bacteria liquid, microscopic examination shows that blastospores begin to ger...

Embodiment 3

[0028] Preparation of Cordyceps sinensis liquid medium: 30g boiled potato filtrate (weigh 30g peeled potatoes, add appropriate amount of water to boil for 20min, filter, take the filtrate, which is 30g boiled potato filtrate), 1.5g peptone, 3g Maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a 500mL Erlenmeyer flask, and dilute to 150mL with water. After autoclaving, place in a culture room at 12±2°C to pre-cool, add 1mL per 150mL of liquid medium with a concentration of 1.5×10 7 Each / mL blastospore was inoculated into the mother liquor so that the final concentration of blastospore was 10 5 each / mL, and add 20-hydroxyecdysone (without adding 20-hydroxyecdysone as a control) to make the final concentration 1mM, place it on a shaker with a rotation speed of 100 rpm, and store it at 12±2°C in the dark On the second day of cultivation, take out 1mL of the culture bacteria liquid, microscopic examination shows that blastospores ...

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Abstract

The invention discloses application of 20-hydroxy molting hormone in promoting development of ophiocordyceps sinensis blastospores for generating hyphae. For the problems of long time for the ophiocordyceps sinensis blastospores to develop and form mycelia, long cycle and poor efficiency of artificial culture of ophiocordyceps sinensis and the like, the 20-hydroxy molting hormone is added in an ophiocordyceps sinensis culture medium to perform hypha induction culture on the blastospores, it is found that the blastospores germinate to form the hyphae when the culture is performed for 2 days, the blastospores germinate to form long hyphae when the culture is performed for 8 days, and the hypha formation rate is increased by 32.25-171.44% compared with the situation that no inducing substanceis added. The 20-hydroxy molting hormone promotes the growth of the blastospores in the mode of forming the hyphae, and improves the efficiency of the formation of the hyphae through the developmentof the ophiocordyceps sinensis blastospores.

Description

Technical field: [0001] The invention belongs to the technical field of fungal culture, and in particular relates to the application of 20-hydroxyecdysone in promoting the development of spores of Cordyceps sinensis to form hyphae. Background technique: [0002] Cordyceps sinensis Ophiocordyceps sinensis is a traditional precious Chinese herbal medicine in my country. Together with ginseng and antler, it is known as the three treasures of Chinese medicine. Cordyceps sinensis is a complex of dead insects and fungal sporosomes formed by infecting the larvae of the bat moth family Hepialidae by Cordyceps sinensis fungus (also known as Mortierella sinensis, Trichosporum sinensis). Cordyceps sinensis has a special habitat (it only reproduces naturally in the high-altitude alpine meadow environment), with slow growth rate, long cycle and extremely limited natural resources. Although Cordyceps sinensis has been listed as a second-class protected species in the "List of National Ke...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 刘桂清曹莉韩日畴
Owner INNER MONGOLIA FURUI MEDICAL SCI
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