A kind of high-purity ellagic acid and its preparation method and application

Abstract

The invention discloses high-purity ellagic acid as well as a preparation method and application thereof. The purity of the ellagic acid is more than 95%. The preparation method comprises the following steps: S1, removing alkali-insoluble substances; S2, removing water-soluble substances and carrying out acid precipitation treatment; and S3, separation of other organic acid substances: adding thesolid substance prepared by the operation into a sodium carbonate solution with the pH value of 7.3-7.8, standing the solution, collecting sediments from the bottom, and washing the sediments twice ormore by using a 20% ethanol water solution to obtain the high-purity ellagic acid. According to the scheme, phenolic acid impurities in the ellagic acid crude product are removed by utilizing the difference between the Ksp of the impurities and the Ksp of the ellagic acid, so that the purity of the ellagic acid is improved; 20% ethanol is used for water-washing operation, so that the loss of ellagic acid is greatly reduced. The method is simple and convenient to operate, mild in reaction condition and suitable for industrial large-scale production. The ellagic acid is good in yield and high in purity.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a high-purity ellagic acid and a preparation method and application thereof. Background technique [0002] Ellagic acid is a polyphenol dilactone, a dimerized derivative of gallic acid, CAS No. 476-66-4, refractive index 1.5800, melting point ≥ 360 °C, molecular formula C 14 H 6 O 8 , boiling point ≥ 420 ℃, molecular weight 302.19g / mol. Ellagic acid is widely found in various soft fruits, nuts and other plant tissues, and belongs to natural polyphenols. Studies have shown that ellagic acid has strong free radical scavenging and antioxidant capabilities. Ellagic acid has a scavenging effect on oxygen free radicals and hydroxyl radicals, and its ability to scavenge free radicals is higher than that of sesamol, olive leaf extract, lutein and cyanidin. In the prior art, relevant studies have shown that ellagic acid has a significant inhibitory effect on chemic...

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Problems solved by technology

(2) High Performance Liquid Chromatography (HPLC) (especially Reverse Phase-High Performance Liquid Chromatography (RP-HPLC)) is currently the most common method for determining the content of ellagic acid method, the method is simple to operate, requires less sample volume, short measurement time, accurate experimental results and good reproducibility, but the required instruments are relatively expensive

Although this method can produce high-purity ellagic acid products, the operation is relatively complicated and the technical requirements for the resin are relatively high.