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Method for extracting active peptide from scapharca subcrenata

A technology of active polypeptides and cockles, applied in the field of active polypeptides, can solve the problem of low extraction rate of active polypeptides in cockles, and achieve the effects of strong regulation of human immunity, promotion of lymphocyte proliferation, and promotion of T or B lymphocyte proliferation

Inactive Publication Date: 2019-08-23
兰溪市哥特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional Chinese medicine believes that its nature is warm and sweet, and it belongs to the spleen and liver meridian. It has the effects of warming the stomach, nourishing yin and nourishing blood. In the technology, the extraction rate of the active polypeptide of the clam is not high, and an extraction method is needed to improve the current situation that the extraction rate of the active polypeptide of the clam is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A method for extracting active polypeptides from cockles. The extraction steps of active polypeptides in cockles are as follows: take fresh cockles and shell them, add distilled water, and homogenate. The solid-liquid ratio of the homogenate is 1:1.3, and the distilled water contains 0.74wt% D- The mixture of mandelic acid and L-mandelic acid is soaked with 0.14mol / L NaOH and 0.34mol / L EDTA-2Na solution respectively, and filtered to obtain filtrate A. In filtrate A, the solid-to-liquid ratio is 1:12 Add 0.25mol / L acetic acid solution and use electric rod to stir and extract. The voltage at both ends of the electric rod is 12V, the stirring time is 11h, the stirring speed is 68r / min, centrifuged at 6200r / min for 8min, and NaCl is added to the supernatant to the final solution. The concentration is 2.5mol / L, and flocculent precipitates are precipitated at the same time, and centrifuged at 9000r / min for 12min at 3°C ​​to obtain the active polypeptide of cockles;

[0013] D...

Embodiment 2

[0015] A method for extracting active polypeptides from cockles, the preferred extraction steps of the active polypeptides of cockles are as follows: take fresh cockles and shell them, add distilled water, homogenate, the solid-liquid ratio of the homogenate is 1:2, and the distilled water contains 0.8wt% D -Mixture of Mandelic Acid and L-Mandelic Acid, soak with 0.14mol / L NaOH and 0.32mol / L EDTA-2Na solution respectively, filter to get filtrate A, in filtrate A by solid-liquid ratio 1: 14 Add 0.28 mol / L acetic acid solution and use electric rod to stir and extract, the voltage at both ends of the electric rod is 10V, the stirring time is 12h, the stirring speed is 68r / min, centrifuge at 6500r / min for 8min, add NaCl to the supernatant to the solution The final concentration was 2.5mol / L, and flocculent precipitates were precipitated at the same time, and centrifuged at 8000r / min for 12min at 2°C to obtain the active polypeptide of clams;

[0016] Distilled water contains a mix...

Embodiment 3

[0018] A method for extracting active polypeptides from cockles, the extraction steps of the active polypeptides of cockles are as follows: take fresh cockles and shell them, add distilled water, homogenate, the solid-liquid ratio of the homogenate is 1:1.8, and the distilled water contains 0.84wt% D- The mixture of mandelic acid and L-mandelic acid is soaked with 0.17mol / L NaOH and 0.34mol / L EDTA-2Na solution respectively, and filtered to obtain filtrate A. In filtrate A, the solid-to-liquid ratio is 1:12 Add 0.24mol / L acetic acid solution and use electric rod to stir and extract. The voltage at both ends of the electric rod is 16V, the stirring time is 11h, the stirring speed is 67r / min, centrifuged at 6200r / min for 10min, and NaCl is added to the supernatant until the final solution The concentration is 2.5mol / L, and flocculent precipitates are precipitated at the same time, and centrifuged at 9000r / min for 13min at 4°C to obtain the active polypeptide of cockles;

[0019] ...

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Abstract

The invention discloses a method for extracting active peptide from scapharca subcrenata. The method comprises the following specific steps that the fresh scapharca subcrenata is collected, subjectedto shell removal and added into distilled water, and through homogenizing processing, soaking and filtration, filtration liquid A is obtained; an acetic acid solution is added into the filtration liquid A, an electric rod is adopted for conducting stirring and extraction, through centrifugal processing, a supernate is collected, NaCl is added into the supernate until the final concentration of themixed solution is 2.5 mol / L, a flocculent precipitate is separated out and centrifuged, and the scapharca subcrenata active peptide is obtained. According to the method, the extracted scapharca subcrenata active peptide is high in activity and extraction rate and contains high-content hydrophobic amino acid.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, in particular to a method for extracting active polypeptides from cockles. Background technique [0002] Hair clam ( Arca subcrenata Lischke ) , mollusk phylum, clambranch class, clam order, clam family, clam genus, mainly distributed in the coastal areas of China, Korea and Japan. The nutritional and medicinal value of cockles are high, and both the shell and the meat have medicinal value. Traditional Chinese medicine believes that its nature is warm and sweet, and it belongs to the spleen and liver meridian. It has the effects of warming the stomach, nourishing yin and nourishing blood. In the technology, the extraction rate of active polypeptides of cockles is not high, and an extraction method is needed to improve the current situation that the extraction rate of active polypeptides of cockles is not high. Contents of the invention [0003] The object of the present inventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 李健
Owner 兰溪市哥特生物技术有限公司
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