Human serum calprotectin detection kit and preparation method thereof

Abstract

The invention belongs to the field of the medical examination, and specifically relates to a detection kit of calprotectin content in human body, and a preparation method thereof. The detection kit comprises a reagent R1 which is latex particles coated with calprotectin monoclonal antibody 1; and reagent R2 which is latex particles coated with calprotectin monoclonal antibody 2; the problems thatthe reagent blank and the reaction amplitude fluctuation are caused since the reagent R1 and the reagent R2 in the existing latex immunoturbidimetry calprotectin detection kit are undermixing in the use process in the use process, and a test result accuracy is influenced since the polyclonal antibody in the reagent R2 has cross reaction with the VIII type collagen, complement C1q and VIII factor are overcome; the reagent R1 and the reagent R2 are latex reagents, the mixing uniformity thereof is improved, and the reagent blank and the reaction amplitude great fluctuation can be avoided; the calprotectin polyclonal antibody used in the traditional reagent is replaced with the calprotectin monoclonal antibody, the extra cross reaction is avoided, and the detection precision and stability of the reagent are improved.

Description

technical field [0001] The invention belongs to the field of medical examination, and in particular relates to a detection kit for calprotectin content in a human body and a preparation method thereof. Background technique [0002] Calprotectin is a small molecular protein complex secreted by activated neutrophils, and the level of calprotectin reflects the activation degree of neutrophils. In recent years, studies have confirmed that the level of calprotectin is closely related to the degree of inflammatory activity in autoimmune diseases such as rheumatism and inflammatory bowel disease (IBD). standard". However, there are shortcomings such as poor patient compliance and unstable test results in the detection of fecal calprotectin. Serum calprotectin measurement is a better choice to avoid these shortcomings, and foreign researchers have confirmed it by ELISA method. [0003] Enzyme-linked immunoassay has high sensitivity, but the operation is cumbersome, and it is diffi...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the uneven mixing of reagents R1 and R2 in the human serum calprotectin detection kit of the latex immunoturbidimetric method in the prior art, resulting in blank reagents and fluctuations in the response range. There is a defect that the polyantibody in reagent R2 in the technology will cross-react with type VIII collagen, complement C1q and factor VIII, thus affecting the accuracy of the final test result. By improving the formula of R1 and R2, reagent R1 and reagent R2 are combined Set as a latex reagent and cross-link calprotectin monoclonal antibody on R1 and R2 respectively, thereby improving the mixing of the two, greatly improving its stability and reagent performance

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