Establishment method of diarrhea-type irritable bowel syndrome compound animal model

A technology of irritable bowel syndrome and animal models, which is applied in the field of medical research, can solve problems such as complicated pathogenesis, and achieve the effect of good experimental response, sensitive feeling and convenient experiment

Pending Publication Date: 2019-08-30
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the animal models of IBS disease syndromes only focus on one aspect of the pathophysiological mechanism, but the pathogenesis of IBS is complex, and the inducing factors are individualized and diversified, resulting in the current animal models having varying degrees of limitations.

Method used

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  • Establishment method of diarrhea-type irritable bowel syndrome compound animal model
  • Establishment method of diarrhea-type irritable bowel syndrome compound animal model
  • Establishment method of diarrhea-type irritable bowel syndrome compound animal model

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Experimental program
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Embodiment

[0026] 1. Experimental content

[0027] 1.1 Experimental animals Sprague-Dawley rats were provided by Shanghai Xipuerbikai Experimental Animal Co., Ltd., with the certificate number SCXK (Shanghai) 2008-0016, and were fed by the clean-level feeding room of the Experimental Animal Center of Jiangsu Academy of Traditional Chinese Medicine. The temperature was Indoor 22-26 ℃, natural light, free to eat and drink.

[0028] 1.2 Experimental Equipment Reagents MICROM-340E paraffin slicer from German ZEISS company, HYPERCENTER-XP tissue dehydrator from British SHANDON company, HISTOCENTRE-2 tissue embedding machine from British SHANDON company, and LOGENEPA9000 pathological image analysis system. Reagent: SERT antibody (1:200) of Abcam Company, UK, batch number: ab65446.

[0029] 1.3 Animal modeling

[0030] The IBS-D rat model was established by neonatal colorectal stimulation combined with adult senna gavage. After the Sprague-Dawley pregnant rats were bought back, they were rai...

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Abstract

The invention discloses an establishment method of a diarrhea-type irritable bowel syndrome compound animal model. The establishment method specifically comprises the steps of repurchasing Sprague-Dawley pregnant rats for predelivery, and recording the delivery date. The method comprises the procedures of 1, colorectum stimulation in the neonatal stage, wherein colorectum stimulation is separatelyconducted in the 8 th day, the 10 th day and the 12 th day after the birth of modeling groups of young rats, a percutaneous transluminal coronary angioplasty balloon is inserted in the colorectum viathe anus, after the balloon completely enters the colorectum, gas is injected into the balloon, so that the pressure in the balloon is 60 mmHg and is kept for 1 minute, stimulation is conducted onceevery day and conducted three times in total, after stimulation, the young rats and mother rats are fed together to the 22 nd day and then weaned, cage allocation is conducted on the 30 th day, then normal feeding is conducted, 60 days after the birth, abdominal wall withdrawal reflex examination is conduced, intestinal-tract high-sensitivity rats are screened out, and the next step of modelling is conducted; 2, gavage with folium sennae juice, wherein folium sennae is put into boiled water which is 5 times the volume of the folium sennae to be soaked for one night, after filtering, a filtrateis taken, and then concentrated until the concentration is 0.45 g/mL, the 0.45 g/mL folium sennae juice is fed for gavage of the screened-out 60-day intestinal tract high-sensitivity rats, 2 mL of the juice is fed to each rat once every day, gavage is continuously conducted for 2 weeks, and then the IBS-D rat model is established.

Description

technical field [0001] The invention belongs to the field of medical research, and relates to a method for establishing an animal model, that is, a method for establishing an animal model for diarrhea-type irritable bowel syndrome. Background technique [0002] Diarrhea-predominant irritable bowel syndrome can be classified as "abdominal pain" and "diarrhea" in traditional Chinese medicine. The etiology includes many aspects such as feeling external pathogens, eating disorder, emotional disorder and so on. The main TCM pathogenesis is stagnation of the liver and deficiency of the spleen, failure of the liver to ventilate, and dysfunction of the spleen in movement, involving the liver, heart, spleen, stomach, kidney, large and small intestine and other viscera. Feeling external pathogens: "Su Wen Ju Pain Theory": "Cold air stays between the intestines and stomach, under the membrane, the blood cannot disperse, and the small collaterals are urgently drawn, so it hurts." If y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/105A01K2267/035
Inventor 陆敏樊欣钰谢慧
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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