Skin care lotion and preparation method thereof
A technology for skin care lotion and supernatant, which is used in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve problems such as pollution, side effects, environment, lack of ability to eliminate acne marks, etc., to eliminate acne marks and repair skin. effect of cells
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Embodiment 1
[0044] Preparation of umbilical cord tissue extract:
[0045] (1) Treatment of umbilical cord tissue: take the isolated umbilical cord tissue of a healthy cow after natural delivery after inspection and quarantine, tie the two ends of the umbilical cord tightly with silk thread, soak it in normal saline, and transport it back to the laboratory at 4°C. After cleaning the umbilical cord tissue with sterilized pure water, use sterile surgical scissors to remove the two ends of the umbilical cord, soak in povidone iodine for 1 min, take it out, deiodine it with 75% alcohol, rinse it with sterilized pure water for 3 times, and mechanically remove it under sterile conditions. Formula homogenate.
[0046] (2) Enrichment of extract: Add 30 times volume of sterilized pure water and 3 times volume of trypsin with a concentration of 2.5g / L to the homogenate tissue obtained in step (1) to make a mixed solution, and the The above mixture was incubated in an anaerobic incubator at 40° C. f...
Embodiment 2
[0061] Preparation of umbilical cord tissue extract:
[0062] (1) Treatment of umbilical cord tissue: take the isolated umbilical cord tissue of a healthy cow after natural delivery after inspection and quarantine, tie the two ends of the umbilical cord tightly with silk thread, soak it in normal saline, and transport it back to the laboratory at 4°C. After cleaning the umbilical cord tissue with sterilized pure water, remove both ends of the umbilical cord with sterile surgical scissors, soak in povidone iodine for 3 minutes, take it out, deiodine with 60% alcohol, rinse once with sterilized pure water, and mechanically clean it under sterile conditions. Formula homogenate.
[0063] (2) Enrichment of the extract: Add 1 volume of sterilized pure water and 1 volume of trypsin with a concentration of 1 g / L to the homogenate tissue obtained in step (1) to make a mixed solution, and the The mixture was incubated in an anaerobic incubator at 45° C. for 24 hours without adding any ...
Embodiment 3
[0078] Preparation of umbilical cord tissue extract:
[0079] (1) Treatment of umbilical cord tissue: take the isolated umbilical cord tissue of a healthy cow after natural delivery after inspection and quarantine, tie the two ends of the umbilical cord tightly with silk thread, soak it in normal saline, and transport it back to the laboratory at 4°C. After cleaning the umbilical cord tissue with sterilized pure water, remove both ends of the umbilical cord with sterile surgical scissors, soak in povidone iodine for 5 minutes, take out, deiodine with 90% alcohol, rinse 5 times with sterilized pure water, and mechanically Formula homogenate.
[0080] (2) Enrichment of the extract: the homogenate tissue obtained in step (1) is added with 50 times the volume of sterilized pure water and 5 times the volume of trypsin with a concentration of 4g / L to make a mixed solution, and the The mixture was incubated in an anaerobic incubator at 35° C. for 72 hours without adding any medium t...
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