A PCR-SBT method and reagents for human killer cell immunoglobulin receptor kir3dl2 genotyping

Abstract

The present invention belongs to the technical field of genotyping detection methods, particularly relates to a PCR-SBT method for genotyping of human killer cell immunoglobulin receptor KIR3DL2 and also relates to a reagent for genotyping of the human killer cell immunoglobulin receptor KIR3DL2. The provided reagent and method can be used as an independent and widely-applied identification method, solve a problem of accurate typing of the KIR3DL2, and exert characteristics of simple and convenient operation and accurate results of the typing of the KIR3DL2 by PCR-SBT. Relevant applications inthe fields of clinical transfusion medical research, genetics, etc. are highly emphasized, and the method and the reagent have important practical significance for medical research units, pharmaceutical research and reagent development units.

Description

technical field [0001] The invention belongs to the technical field of genotyping detection methods, in particular to a PCR-SBT method for human killer cell immunoglobulin receptor KIR3DL2 genotyping. The invention also relates to a human killer cell immunoglobulin receptor KIR3DL2 genotyping reagent. Background technique [0002] Killer cell immunoglobulin-like receptors (KIR) belong to the immunoglobulin-like superfamily, mainly expressed on the surface of NK cells and T cells, and conduct inhibition or activation by interacting with HLA class I molecules (ligands) on the surface of target cells Signals, thereby regulating the inhibitory and killing activities of NK cells and T cells, play an important role in physiological and pathological processes such as tumor immunity, elimination of aging mutant cells, anti-infection immunity, maternal-fetal tolerance, organ transplantation, and autoimmune diseases. KIR3DL2 belongs to the inhibitory KIR gene. Studies have shown that...

AI Technical Summary

Problems solved by technology

At present, the common typing techniques for laboratory detection of KIR3DL2 gene are PCR-SSP, PCR-SSOP and PCR-SBT methods. The first two methods can only provide low-resolution results, and the allele type cannot be determined yet; The method can provide high-resolution results, but due to the particularity of the KIR gene, more than one allele may exist at the same gene locus, and the KIR gene family is highly similar, and there is a certain difficulty in high-resolution KIR detection. Only a few foreign laboratories carry out high-resolution research on the KIR3DL2 gene

The existing PCR-SBT method only targets some exons of the KIR3DL2 gene. With the continuous discovery of new alleles of KIR3DL2, if the previous method is not improved and upgraded in time, there is a possibility of missed detection and false detection

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