Application of red clover as haemophilus parasuis vaccine adjuvant

A technology of Haemophilus suis and red clover, which is applied in the field of application of red clover as an adjuvant for Haemophilus parasuis vaccine, can solve the problems of increasing difficulty and the influence of propolis adjuvant application and promotion, and achieve optimal addition amount and increase Immunity of the body and the effect of increasing antibody titer

Pending Publication Date: 2019-10-18
LONGYAN UNIV
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  • Application of red clover as haemophilus parasuis vaccine adjuvant
  • Application of red clover as haemophilus parasuis vaccine adjuvant
  • Application of red clover as haemophilus parasuis vaccine adjuvant

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[0015] Example 1

[0016] Red clover extracts are selected as adjuvants to prepare adjuvant vaccines containing different concentrations of red clover (polyvalent seedlings + red clover high concentration, polyvalent seedlings + red clover medium concentration, polyvalent seedlings + red clover low The concentration and blank control are shown in Table 1). The piglets were injected with 2 mL of adjuvant-containing polyvalent inactivated vaccine into the neck muscle at the age of 15 days. 21 days after the first immunization, the piglets of the test group were immunized for the second time. The immunization route and dosage were the same. For the first immunization, 14 days after the second immunization, the test group piglets were challenged with Haemophilus parasuis serotype 4 and 5, the challenge method was intraperitoneal injection of 3ml bacteria per head, and the content of type 4 and 5 live bacteria were both 7.5×10 9 CFU, a multivalent vaccine and a blank control were set ...

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Abstract

The invention discloses an application of a red clover as a haemophilus parasuis vaccine adjuvant. Bioactive substances obtained by extraction, separation and purification from Chinese medicinal herbsnamely red clover, mainly contain various components of flavonoid, amino acids, polysaccharide and the like, and the haemophilus parasuis vaccine adjuvant has the effects of resisting oxidation, preventing osteoporosis, resisting cancer, promoting animal growth and improving immunity of organisms. The haemophilus parasuis vaccine adjuvant is combined with a haemophilus parasuis multivalence inactivated vaccine, so that an aemophilus parasuis red clover adjuvant inactivated vaccine is prepared, and the additive amount of the red clover is optimized. After immunization twice, the antibody titerof aemophilus parasuis Hps can be effectively increased, and the IL-2 and IL-4 level of pigs can be effectively increased. The invention provides a novel vaccine and an immunization method for the haemophilus parasuis.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to the application of red clover as an adjuvant for Haemophilus parasuis vaccine. Background technique [0002] At present, traditional vaccines, such as Haemophilus parasuis vaccine, mostly use aluminum gel, mineral oil, and propolis as vaccine adjuvants. [0003] However, aluminum salts are not a good adjuvant in many situations, especially in inducing cellular immune responses. Granulomas are commonly produced when aluminum adjuvants are injected subcutaneously or intradermally rather than intramuscularly. Another side effect is increased IgE production, allergic reactions and possible neurotoxicity. Mineral oil adjuvants can produce a series of side effects due to the disadvantage of being unable to be metabolized, including inflammatory reactions at the injection site, granulomas, ulcers, and fever. In addition, mineral oil and its components such as p...

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Application Information

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IPC IPC(8): A61K39/39A61P31/04A61P37/04
CPCA61K39/39A61P31/04A61P37/04A61K2039/55588
Inventor 李晓华杨小燕邱龙新郑新添杨守深刘栋
Owner LONGYAN UNIV
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