Leucobacter chromiireducens and application thereof in reducing hexavalent chromium

A technology of bacillus albicans and hexavalent chromium, applied in the field of environmental microorganisms, can solve the problem of low tolerance to hexavalent chromium, and achieve the effect of wide application range

Active Publication Date: 2019-11-15
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently isolated bacteria have low tolerance to hexavalent chromium, which limits the promotion and application of microbial reduction in high chromium environments.

Method used

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  • Leucobacter chromiireducens and application thereof in reducing hexavalent chromium
  • Leucobacter chromiireducens and application thereof in reducing hexavalent chromium
  • Leucobacter chromiireducens and application thereof in reducing hexavalent chromium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Isolation and Identification of Super Hexavalent Chromium-tolerant Bacteria

[0033] Step 1: Bacteria Isolation

[0034] Retrieve the wastewater sample from the wastewater outlet of the tannery, enrich and cultivate it, separate the effective strains on the plate, pick the obligate strains for pure culture, and gradually increase the concentration of hexavalent chromium to obtain Bacteria with strong tolerance to hexavalent chromium.

[0035] Step 2: Colony Morphological Observation

[0036] The strain Leucobacter chromiireducens LYC-2 was streaked and separated on the LB plate, and the morphological characteristics of the colony of the strain on the solid plate were observed after 48 hours: the colony was round, milky yellow, smooth, and the diameter of the colony was 0.5-1.0mm ( figure 1 ).

[0037] Step 3: Gram staining of bacteria

[0038] The bacterial body was sliced, and Gram staining was carried out, and the Gram staining of the bacterial strain w...

Embodiment 2

[0057] The hexavalent chromium tolerance of embodiment 2 bacteria

[0058] Activation of the first step bacteria

[0059] Pick a single colony from the LYC-2 plate and culture it overnight in 20 mL of LB liquid medium, and then transfer it to 100 mL of LB liquid medium for expansion.

[0060] Hexavalent chromium tolerance of bacteria in the second step

[0061] Inoculate the bacterial solution expanded to OD600=0.8-1.0 into LB medium containing different concentrations (1-35g / L) of Cr(VI) at a volume ratio of 10%, and take samples at intervals to measure OD600 to detect For the growth of bacteria, draw the growth curve of bacteria under different concentrations of Cr(VI).

[0062] The results show that: Leucobacter chromiireducens LYC-2 grows well under the conditions of aerobic, normal pressure, 30℃, pH=9, when the concentration of Cr(VI) is 0-20g / L, LYC-2 grows well; when the concentration is 25- When the concentration was 30g / L, the growth amount began to decrease, but n...

Embodiment 3

[0063] Application of Example 3 Bacteria in Hexavalent Chromium Reduction

[0064] Activation of the first step bacteria

[0065] With the first step in embodiment 2.

[0066] The second step is bacterial reduction of hexavalent chromium

[0067] Inoculate the bacterial solution expanded to OD600=0.8-1.0 into LB medium containing different concentrations (50-500mg / L) of Cr(VI) at a volume ratio of 10%, and take samples at intervals to determine the remaining medium The concentration of hexavalent chromium, the reduction of hexavalent chromium by bacteria has been detected, and the reduction curves of bacterial reduction of hexavalent chromium under different concentrations of Cr(VI) are drawn.

[0068] The results show that: Leucobacter chromiireducens LYC-2 can completely reduce 100 mg / L of hexavalent chromium within 48 hours and 100% of 300 mg / L of hexavalent chromium in 96 hours under the conditions of aerobic, normal pressure, 30°C and pH=9. Chromium, showing a strong a...

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Abstract

The invention relates to leucobacter chromiireducens and application thereof in reducing hexavalent chromium, and belongs to the technical field of environmental microorganisms. The provided leucobacter chromiireducens LYC-2 is separated from tannery wastewater and preserved in the China General Microbiological Culture Collection Center in October 19th, 2018 with the preservation number of CGMCC NO.16823. The tolerance capability of the strain to the hexavalent chromium reaches 30 g/L, the processes of culturing the strain LYC-2 and reducing the hexavalent chromium can be performed under aerobiotic and normal-pressure conditions with a wide temperature range of 25-35 DEG C and a wide pH range of 7-11, and the high-toxicity hexavalent chromium can be effectively reduced to low-toxicity trivalent chromium. A culture method of the strain is simple, and the strain is quickly bred, has a short cycle and has import application prospects in the treatment of hexavalent chromium pollution.

Description

technical field [0001] The invention relates to a white bacillus and its application in reducing hexavalent chromium, belonging to the technical field of environmental microorganisms. Background technique [0002] Chromium is widely used in chromium salt manufacturing, electroplating, printing and dyeing, leather tanning, wood preservation, oil extraction, etc. In these production processes, a large amount of chromium-containing waste residue and wastewater are generated, which seriously threaten human health. According to reports, drinking water contaminated by chromium can cause diarrhea; chromium can also cause allergic dermatitis or eczema; chromium also has a certain corrosive effect on the respiratory tract; chromium is also a carcinogenic and mutagenic factor. [0003] In nature, chromium usually exists in two forms: trivalent chromium (Cr(III)) and hexavalent chromium (Cr(VI)), which can be transformed into each other under certain conditions. Trivalent chromium is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C02F101/22C12R1/01
CPCC02F3/02C02F3/34C02F2101/22C12N1/205C12R2001/01Y02W10/10
Inventor 柴立元丁春连黎佳未杨志辉石岩
Owner CENT SOUTH UNIV
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