Application of macamide compound or salt of macamide compound to preparation of drugs for preventing or treating liver fibrosis diseases
A technology of macamide and liver fibrosis, which is applied in the field of medicine, can solve the problems such as failure to effectively reverse liver fibrosis in patients, failure to develop anti-hepatic fibrosis drugs, etc.
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[0050] Example 1 Mice liver fibrosis induced by left middle common bile duct ligation (LMBDL)
[0051] Experimental materials: C57L / 6 mice, 3% sodium pentobarbital solution, surgical instruments.
[0052] experimental method:
[0053] LMBDL group: 12-week-old male C57BL / 6 mice were anesthetized by intraperitoneal injection of 3% sodium pentobarbital solution, cut the abdominal skin and muscle layer, free the common bile duct, ligate the left middle common bile duct, and suture the abdominal cavity.
[0054] Sham operation group: 12-week-old male C57BL / 6 mice were anesthetized by intraperitoneal injection of 3% sodium pentobarbital solution, cut the abdominal skin and muscle layer, free the common bile duct, and suture the abdominal cavity.
[0055] Prevention group: Take mice, calculate according to 25mg / kg / d, 0.25mg / 10g body weight, calculate 20g per mouse, take 0.5mg, that is 20μl of formula (I) N-benzyl sixteen carbon N-benzylhexadecanamide (NBH) liquid (12.5mg powder, dissolved in ...
Example Embodiment
[0059] Example 2 HE staining and Sirius Red Staining observe the effect of macaamide on liver fibrosis induced by common bile duct ligation in mice
[0060] Experimental materials: camera, tissue section making, microscope.
[0061] Experimental method: The mouse liver tissue was fixed with paraformaldehyde, dehydrated, transparent, and waxed, then embedded in paraffin with an embedding machine, and stored in a refrigerator at 4°C after the wax block had solidified. Then the wax block was made into paraffin sections for HE staining and Sirius red staining.
[0062] HE staining: deparaffinize with xylene Ⅰ and Ⅱ for 10 minutes each; put the sections in 100%, 95%, 85%, 75% alcohol solutions for 10 minutes each, rinse with tap water for 5 minutes, 1×PBS 5 After 30 minutes incubation with 0.3% H2O2 37℃, wash again with 1×PBS for 5 minutes; staining: hematoxylin dip staining for 2 minutes, washing with tap water for 10 minutes, differentiation with 1% hydrochloric acid and ethanol for 5 ...
Example Embodiment
[0065] Example 3 The effect of macaamide on alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mice with liver fibrosis induced by common bile duct ligation.
[0066] Experimental materials: Macaamide, surgical instruments, high-speed centrifuges and biochemical detectors, etc.
[0067] Experimental method: The mice in the sham operation group, the LMBDL group, the macaamide prevention group, and the normal control group were subjected to orbital blood sampling and centrifugation (4000rpm, 5min), and the supernatant was taken and sent to the laboratory of the hospital for serum detection using a biochemical detector Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) content.
[0068] Experimental results: Compared with the normal control group, the ALT and AST of hepatic fibrosis mice induced by common bile duct ligation were significantly increased, and macaamide can significantly alleviate the above phenomenon, as shown in Table 1 below:
[0069]...
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