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Bladder cancer detection kit and application thereof

A detection kit, bladder cancer technology, applied in the field of bladder cancer detection kits, to achieve automatic result judgment, high sensitivity, and good detection effect

Active Publication Date: 2019-12-06
CANCER CENT OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic patents on exosomes mainly focus on the separation and detection of exosomes, but there are few public reports on the use of exosomes extracted from urine for auxiliary diagnosis of bladder cancer. Only Zhejiang University has published a urine exosome An integrated detection method and detection chip for body separation, enrichment and detection. By detecting cancer information in urine exosomes, it is expected to be used for non-invasive diagnosis and monitoring of bladder cancer. This method uses the unique transmembrane protein CD63 of exosomes A rapid ELISA method for the diagnosis of bladder cancer was established, but the screening of bladder cancer-specific tumor markers was not carried out

Method used

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  • Bladder cancer detection kit and application thereof
  • Bladder cancer detection kit and application thereof
  • Bladder cancer detection kit and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0062] 1 Urine sample collection and pretreatment: 125 cases of bladder cancer patients (patients before treatment) and 146 cases of controls (including 50 cases of healthy people, 96 cases of patients with benign urinary system diseases) urine samples, a total of 271 cases of urine samples. The urine is morning urine or urine that has been held for more than 2 hours, with a volume of more than 15ml. After collection, it should be sent for inspection in time, and the storage time at room temperature should not exceed 2 hours. Urine samples were centrifuged at 2000 g for 10 min, and the supernatant was transferred to a new centrifuge tube for exosome extraction. The sediment was examined by Wright's staining for urine exfoliation cytology.

[0063] 2 Separation of urinary exosomes: PEG precipitation method was used to separate exosomes. 15-20ml of urine supernatant, 3000g, centrifuge at 4 degrees for 15min, take the supernatant into another new centrifuge tube, add PEG8000 wi...

Embodiment 2

[0085] Four miRNAs diagnostic performance evaluation of embodiment 2

[0086]The diagnostic ability of four serum miRNAs to distinguish bladder cancer patients from controls was compared by ROC curve analysis. The areas under the ROC curve (AUC) of the four miRNAs miR-423-5p, miR-203-3p, miR-21-5p, and miR-30b-5p were: 0.880 (95% CI 0.836-0.917), 0.703 (95% CI 0.645-0.757), 0.799 (95% CI 0.746-0.845), 0.850 (95% CI 0.802-0.890), see figure 2 . miR-423-5p has a sensitivity of 69.9% and a specificity of 92.8%; miR-203-3p has a sensitivity of 63.7% and a specificity of 76.0%; miR-21-5p has a sensitivity of 65.8% and a specificity of 91.2%; miR-30b-5p has a sensitivity of 69.9% , specificity 86.4%. For more accurate prediction of bladder cancer, a combination of biomarkers is a better choice. The joint diagnosis model was established by logistic regression and neuron algorithm. The combined sensitivity and specificity of 4 miRNA markers (miR-423-5p, miR-203-3p, miR-21-5p, mi...

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Abstract

The invention provides a bladder cancer detection kit and application thereof. By detecting expression of at least one of miR-423-5p, miR-203-3p, miR-21-5p and miR-30b-5p in exosomes from tumors, thekit and system can be used for detecting tumors by utilizing urine which is a biological sample and are high in stability and accurate in quantification. The kit is rapid, low in cost, high in accuracy and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a bladder cancer detection kit and its application. Background technique [0002] Bladder cancer refers to the most common malignant tumor of the urinary system that occurs on the bladder mucosa, with an average age of onset of about 65 years, of which about 90% are transitional cell carcinoma, and the other 10% are mainly squamous cell carcinoma and adenocarcinoma. The incidence rate of bladder cancer in the national tumor registration area is 6.61 / 100,000, ranking ninth in the incidence rate of malignant tumors. Due to the obvious characteristics of poor prognosis and easy recurrence, and the 5-year survival rate of bladder cancer patients with early detection and early treatment is greater than 90%, early diagnosis is of great significance to the treatment of this disease. Cystoscopy is currently the most reliable method for diagnosing bladder cancer....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/686C12N15/113
CPCC12Q1/6886C12Q1/686C12Q2600/178C12Q2563/107C12Q2545/114
Inventor 邓敏刘万里
Owner CANCER CENT OF GUANGZHOU MEDICAL UNIV
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