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SNP Molecular Markers Related to Stripe Rust Resistance Genes and Their Primers and Applications in Adult Plant Stage of Wheat

A technology of wheat stripe rust and molecular markers, applied in the field of agricultural biology, can solve the problems of cumbersome technology, limit the application of plant breeding, and high test costs, and achieve the effects of good repeatability, accelerated breeding process, and strong stability

Active Publication Date: 2022-05-31
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many detection methods for SNP, such as: DNA chip detection, DNA sequencing method, denaturing high performance liquid chromatography, flight mass spectrometer detection, etc., but most of them are limited in application in plant breeding due to cumbersome techniques and high test costs.

Method used

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  • SNP Molecular Markers Related to Stripe Rust Resistance Genes and Their Primers and Applications in Adult Plant Stage of Wheat
  • SNP Molecular Markers Related to Stripe Rust Resistance Genes and Their Primers and Applications in Adult Plant Stage of Wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Immunity (0): no symptoms were produced on the leaves;

[0037] High sense (4): the leaves are not chlorotic, and a large number of necrospore piles are grown on the top.

[0039] Using the CTAB method to extract genomic DNA.

Embodiment 2

[0046] According to the SNP molecular marker sequence, obtain a wheat DNA sequence with a matching degree of 100% with the target sequence.

Embodiment 3

[0070] With embodiment 1.

[0072] With embodiment 2.

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PUM

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Abstract

The invention belongs to the field of agricultural biotechnology, and in particular relates to a SNP molecular marker related to a wheat stripe rust resistance gene at the adult plant stage, a specific primer and application thereof. The SNP molecular marker related to the stripe rust resistance gene at the adult plant stage of wheat has a nucleotide sequence shown in SEQ ID NO.1, wherein the 36th base in the sequence shown in SEQ ID NO.1 is A or G. The molecular marker of the present invention can be used for auxiliary selective breeding of wheat stripe rust resistance, and can overcome the vulnerability of the identification of wheat stripe rust resistance to environmental influence, poor stability and repeatability, large workload, time-consuming and laborious, and long breeding cycle in conventional breeding and other shortcomings, thereby accelerating the breeding process of wheat varieties resistant to stripe rust.

Description

SNP Molecular Markers Related to Stripe Rust Resistance Gene in Adult Stage of Wheat and Their Primers and Applications technical field The invention belongs to the field of agricultural biotechnology, be specifically related to the related SNP molecule of wheat adult plant stage resistance to stripe rust gene Markers and their specific primers and applications. Background technique [0002] Wheat is an important food crop in my country, and its quality and output are directly related to national food security issues. Depend on Puccinia striiformis Westend.f.sp.tritici (Pst) can spread over long distances with high air currents Wheat stripe rust caused by it is one of the typical airborne diseases with the most serious damage to wheat. It has the characteristics of high speed and serious damage. China is the largest and relatively independent wheat in the world with the occurrence of wheat stripe rust. The endemic areas of stripe rust mainly occur in Sichuan, Guizh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156Y02A40/10
Inventor 陈国跃吴郁魏育明郑有良蒋云峰康厚扬江千涛马建祁鹏飞
Owner SICHUAN AGRI UNIV
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