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Molecular marker linked to introgression line's stripe rust resistance site from Agropyron P genome in adult-plant stage and application thereof

A technology for molecular markers and stripe rust, which is applied in special data processing applications, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of general limitation, marker target gene far away, and few applications, so as to speed up the breeding process , close genetic distance and good repeatability

Active Publication Date: 2018-01-16
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The SNAP marker technology has the advantages of simplicity, rapidity, high stability and high allelic diversity. At present, multiple stripe rust resistance genes have been successfully screened and identified by using this technology. Rust resistance gene-linked markers, the versatility is often limited by the variety background, and most of the markers are far away from the target gene, so these markers are not widely used in breeding

Method used

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  • Molecular marker linked to introgression line's stripe rust resistance site from Agropyron P genome in adult-plant stage and application thereof
  • Molecular marker linked to introgression line's stripe rust resistance site from Agropyron P genome in adult-plant stage and application thereof
  • Molecular marker linked to introgression line's stripe rust resistance site from Agropyron P genome in adult-plant stage and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Obtaining the SNAP molecular marker 2B_SNAP_102 linked to the resistance gene of adult plant stage of stripe rust from the introgression line of wheatgrass P genome

[0029] 1. Test materials

[0030] Plant materials: the adult plant resistant wheat-Agrocybe introgression line Pubing 3558 was used as the female parent, and the susceptible variety Avocet S was used as the male parent to construct F 2 Genetic Mapping Populations. Planting will F 2 152 individual plants from the population were used for resistance identification. Some leaves of each individual plant were taken at the seedling stage for SNAP analysis. When ripe, harvest F 2:3 The seeds of the family were dried and preserved for resistance identification.

[0031] Physiological race of stripe rust: for F 2 group and F 2:3 The physiological race of P. stripe rust identified by family resistance is the purified physiological race 33 in Tiaozhong.

[0032] 2. Identification of resistance to s...

Embodiment 2

[0047] Example 2: Molecular-assisted selection of wheat stripe rust resistance gene at adult plant stage

[0048] 1. Test materials

[0049] Plant materials: 7 wheat lines (L1-L7) of the advanced generation (F7) of the hybrid combination of the disease-resistant parent Pubing 3558 and the susceptible line SY95-71, 5 disease-resistant Sichuan local wheat varieties (Lezhihong Tiaomai, Deyangtian Lvchang wheat, Gusong Dalongxu, Fengdu Pushanmai, Zhongjiang Aizi Huoshaomai) and 5 wheat cultivars unrelated to the disease-resistant parent Pubing 3558 (Chuanmai 33, Chuanmai 61, Mianmai Mai 41, Mianmai 1403, Zhongkemai 47) The above materials were planted in the Chongzhou Experimental Base of Sichuan Agricultural University in the first season of 2016 for the identification of stripe rust resistance in adult plants. Some leaves of each individual plant were taken at the seedling stage for SNAP analysis.

[0050] Mixed physiological races of stripe rust: The physiological races of st...

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Abstract

The invention relates to the field of molecular marker, specifically to a molecular marker linked to introgression line's stripe rust resistance site from Agropyron P genome in adult-plant stage and application thereof. The size of the molecular marker is 110 bp, and the molecular marker is located on the long arm of wheat chromosome 2B with the genetic distance being 1.0 cM, has a short genetic distance from resistance gene, has good PCR amplification repeatability and strong stability, can be used for variety identification of wheat stripe rust and molecular marker-assisted selection of wheat disease-resistant offsprings, and furthermore accelerates breeding process of disease-resistant variety.

Description

technical field [0001] The invention relates to the field of molecular markers, in particular to molecular markers linked with adult plant stripe rust resistance loci from the introgression line of wheatgrass P genome and application thereof. Background technique [0002] Wheat stripe rust caused by Puccinia striiformis Westend.f.sp.tritici (Pst) is one of the most severe typical airborne diseases of wheat. Therefore, it is urgent to discover new stripe rust resistance genes and Efficiently transfer to the existing main cultivars to enhance the resistance of wheat cultivars. [0003] Discovering new stripe rust resistance genes from wheat wild relatives, rare species and local wheat, and effectively using them, breeding and promoting new stripe rust resistance varieties has become an important task in my country's wheat stripe rust resistance breeding. [0004] The SNAP marker technology has the advantages of simplicity, rapidity, high stability and high allelic diversity. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11G06F19/22
Inventor 陈国跃陈思晔叶雪玲姚方杰江千涛马建李伟刘亚西魏育明郑有良
Owner SICHUAN AGRI UNIV
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