A kind of detection method of hordenine hydrochloride

A technology of hordenine hydrochloride and detection method, which is applied in the field of analysis and detection, can solve the problems of unfavorable reaction degree hordenine hydrochloride product application, etc., and achieve avoiding adverse effects, good peak separation effect, and good repeatability Effect

Active Publication Date: 2022-02-25
JING JING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are few reports on the detection method of hordenine hydrochloride content in the conversion liquid at present, which is not conducive to the control of the reaction degree and the application of hordenine hydrochloride products

Method used

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  • A kind of detection method of hordenine hydrochloride
  • A kind of detection method of hordenine hydrochloride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A detection method for hordenine hydrochloride, comprising the steps of:

[0025] S1: Take hordenine hydrochloride reference substance, use mobile phase purified water (adjust pH to 2.3 with phosphoric acid)-acetonitrile (95:5, v / v) as solvent, and prepare concentrations of 10.590 μg / 1ml and 16.944 μg respectively / 1ml, 21.180μg / 1ml, 26.475μg / 1mlh and 33.888μg / 1ml standard solutions;

[0026] S2: Use liquid chromatography to detect the standard solution, and draw a standard curve with the mass concentration of hordenine hydrochloride as the abscissa and the peak area of ​​hordenine hydrochloride as the ordinate to obtain a standard curve regression equation y=3.17×10 7 x+7.44×10 3 , R 2 =0.9998;

[0027] S3: Dilute 0.5ml of the conversion solution containing hordenine hydrochloride to 25ml with the above-mentioned mobile phase, shake it up and then dilute it 50 times to obtain the solution to be tested, and perform chromatographic detection under the same measuremen...

Embodiment 2

[0031] A detection method for hordenine hydrochloride, comprising the steps of:

[0032] S1: Take hordenine hydrochloride reference substance, use mobile phase purified water (adjust pH to 2.0 with phosphoric acid)-acetonitrile (96:4, v / v) as solvent, and prepare concentrations of 10.590 μg / 1ml and 16.944 μg respectively / 1ml, 21.180μg / 1ml, 26.475μg / 1mlh and 33.888μg / 1ml standard solutions;

[0033] S2: Use liquid chromatography to detect the standard solution, and draw a standard curve with the mass concentration of hordenine hydrochloride as the abscissa and the peak area of ​​hordenine hydrochloride as the ordinate to obtain a standard curve regression equation y=3.17×10 7 x+7.44×10 3 , R 2 =0.9998;

[0034] S3: Dilute 0.5ml of the conversion solution containing hordenine hydrochloride to 25ml with the above-mentioned mobile phase, shake it up and then dilute it 50 times to obtain the solution to be tested, and perform chromatographic detection under the same measuremen...

Embodiment 3

[0038] A detection method for hordenine hydrochloride, comprising the steps of:

[0039] S1: Take hordenine hydrochloride reference substance, use mobile phase purified water (adjust pH to 2.5 with phosphoric acid)-acetonitrile (97:3, v / v) as solvent, and prepare concentrations of 10.590 μg / 1ml and 16.944 μg respectively / 1ml, 21.180μg / 1ml, 26.475μg / 1mlh and 33.888μg / 1ml standard solutions;

[0040] S2: Use liquid chromatography to detect the standard solution, and draw a standard curve with the mass concentration of hordenine hydrochloride as the abscissa and the peak area of ​​hordenine hydrochloride as the ordinate to obtain a standard curve regression equation y=3.17×10 7 x+7.44×10 3 , R 2 =0.9998;

[0041]S3: Dilute 0.5ml of the conversion solution containing hordenine hydrochloride to 25ml with the above-mentioned mobile phase, shake it up and then dilute it 50 times to obtain the solution to be tested, and perform chromatographic detection under the same measurement...

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Abstract

The invention relates to the technical field of analysis and detection, and specifically discloses a detection method for hordenine hydrochloride, the detection method comprising the following steps: taking a reference substance of hordenine hydrochloride, using the mobile phase as a solvent, and preparing a series of concentrations of Standard solution; the standard solution is detected by liquid chromatography, and a standard curve is drawn to obtain a standard curve regression equation; the conversion solution containing hordenine hydrochloride is diluted with a mobile phase to obtain a solution to be tested, detected, and recorded According to the peak area, the content of hordenine hydrochloride was obtained according to the regression equation. The detection method provided by the invention is simple in operation, good in repeatability, high in sensitivity and accuracy, good in separation degree, and has a fast peak eluting time, and can quickly and accurately measure the content of hordenine hydrochloride in the transformation liquid.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a detection method of hordenine hydrochloride. Background technique [0002] Hordenine (p-dimethylaminoethylphenol) can act on adrenergic receptors, and its effect is similar to that of ephedrine or ephedrine. It has the effects of relaxing bronchial smooth muscle, constricting blood vessels, increasing blood pressure, raising blood pressure and stimulating the central nervous system. It can be used to relieve bronchitis, bronchial asthma, enhance the tension and movement of the uterus, etc., and has a protective effect on radiation damage. [0003] Hordenine exists in the roots of germinated barley seeds, the roots of leguminous plants, the stems of linden leaf leech, the heartwood of false pigeonpea, etc. It is relatively difficult to separate and the purity of the product is not high. In addition, hordenine is not conducive to long-term storage. If it is expose...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/32G01N30/34G01N30/74
CPCG01N30/02G01N30/06G01N30/32G01N30/34G01N30/74G01N2030/324
Inventor 张思雨武晓山何连顺李斌水
Owner JING JING PHARMA
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