Drug-loaded microspheres loaded with oncolytic virus and preparation method thereof

An oncolytic virus and drug-loaded microsphere technology, applied in the field of biomedicine, can solve the problems of insufficient therapeutic response, complicated administration of oncolytic virus, etc., achieve safe and convenient administration, improve intratumoral replication rate, and time release Effect
CN110638784AInactive Publication Date: 2020-01-03兆科(广州)肿瘤药物有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
兆科(广州)肿瘤药物有限公司
Publication Date
2020-01-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of drug-loaded microspheres loaded with oncolytic virus. The preparation method of the drug-loaded microspheres loaded with the oncolytic virus comprises the following steps: (1) preparing an aqueous-phase gelatin-hydrogel solution; (2) preparing an oil-phase solution; (3) irradiating microsphere precursors made of a mixture of the aqueous phase and theoil phase at a collection end, so that the microsphere precursors cross-link to form no-load microspheres; (4) performing post-treatments, namely greasy-dirtf removing and freeze-drying, on the dulycross-linked no-load microspheres obtained in the step (3); and (5) mixing freeze-dried microspheres obtained in the step (4) with an oncolytic virus solution so as to obtain the drug-loaded microspheres loaded with the oncolytic virus. The preparation method of the drug-loaded microspheres loaded with the oncolytic virus has the following advantages: the microspheres are prepared by adopting bio-compatible materials, including gelatin and the like, and used in intervention of oncolytic virus administration; and thus, immunogenicity of intravenously administrated oncolytic virus can be reducedwith in-tumor replication rate of oncolytic virus improved and anti-tumor ability of a drug enhanced in local hypoxia micro-environment created by the drug-loaded microspheres.
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Description

technical field

[0001] The invention belongs to the technical field of biomedicine, in particular to a drug-loaded microsphere loaded with an oncolytic virus and a preparation method thereof. Background technique

[0002] Oncolytic viruses (OV) refer to a class of native or recombinant viruses that can selectively kill tumor cells without damaging normal cells. Oncolytic virus therapy (Oncolytic virotherapy) is a new type of tumor-targeted therapy. It is an anti-tumor agent that uses tumor-selective and multi-killing oncolytic viruses as its main component. It can directly kill tumor cells to achieve oncolytic effects. , and release tumor-specific antigens in the process of tumor cell lysis, and activate the body's anti-tumor immune response. Currently, oncolytic poxviruses commonly used for genetic modification mainly include Lister strain, WR (Western Reverse) strain, Wyeth strain, Copenhagen strain and Tiantan strain.

[0003] Pexa-Vec (pexastimogene dvacirepvec) virus ...

Claims

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