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Preparation method and detection method of lettuce mosaic virus nano-enzyme immunochromatographic test strip

A technology of immunochromatography test paper and mosaic virus, which is applied in the direction of measuring devices, analysis materials, instruments, etc., can solve the problem of non-universal applicability and detection of lettuce mosaic virus, such as nanoenzyme detection test strip detection method, etc. problem, to achieve the effect of high detection sensitivity

Active Publication Date: 2020-01-10
CHINESE ACAD OF INSPECTION & QUARANTINE
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AI Technical Summary

Problems solved by technology

However, these nanozymes in the prior art do not have universal applicability. For different virus detection, it is necessary to find suitable nanozymes. At present, there are no nanozyme detection test strips and test strips suitable for lettuce mosaic virus. Corresponding detection method

Method used

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  • Preparation method and detection method of lettuce mosaic virus nano-enzyme immunochromatographic test strip
  • Preparation method and detection method of lettuce mosaic virus nano-enzyme immunochromatographic test strip
  • Preparation method and detection method of lettuce mosaic virus nano-enzyme immunochromatographic test strip

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Embodiment Construction

[0044] 1 Materials and methods

[0045] 1.1 Experimental materials

[0046] Purified Lettuce mosaic virus (LMV), Arabismosaic virus (ArMV), Cucumber mosaic virus (CMV), Tobacco ring spot virus (TRSV) , Tomatoringspot virus (ToRSV), Potato virus Y (PVY), Potatovirus A (PVA), Zucchini yellow mosaic virus (ZYMV) and LMV rabbit anti-IgG were all obtained from Chinese Academy of Inspection and Quarantine. The actually tested samples were intercepted by the customs and numbered 1#-14#.

[0047] Biochemical reagents: Goat anti-rabbit IgG purified antibody was purchased from Beijing Lamboride Trading Co., Ltd.; nano-enzyme solution was self-made; polyvinyl chloride plate (PVC), 1-(3-dimethylaminopropyl)-3-ethylcarbodiene Imine (EDC), N-hydroxysuccinimide (NHS), bovine serum albumin (BSA), nitrocellulose membrane (nmilliporehi-Flow135), and absorbent pads are products of Beijing Hongjie Technology Co., Ltd.; diaminobenzidine ( Diaminobenzidine (DAB) chromogenic kit was purchased fr...

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Abstract

The invention discloses a preparation method of a lettuce mosaic virus nano-enzyme immunochromatographic test strip. The preparation method comprises the steps of: sequentially pasting a water absorption pad, a nitrocellulose membrane and a glass fiber membrane on a PVC (Polyvinyl Chloride) bottom plate, and arranging the nitrocellulose membrane between the water absorption pad and the glass fibermembrane; respectively spraying a C line and a T line on the surface of the nitrocellulose membrane of a blank test strip by using an XYZ three-dimensional membrane scratching and gold spraying instrument, and performing drying in a drying oven at a temperature of 37 DEG C, wherein the line C is a goat anti-rabbit IgG antibody line, the T line is an IgG antibody line of rabbit anti-lettuce mosaicvirus LMV; and putting a PVC cover plate on the PCV bottom plate, and forming a color developing window hole and a sample adding hole in the PVC cover plate, wherein the C line and the T line are arranged in the color developing window hole, and the distance between the T line and the sample adding hole is smaller than the distance between the C line and the sample adding hole. The method has theadvantages of relatively high sensitivity, good specificity, low price, short detection time, simplicity in operation and the like, and is suitable for rapid port on-site detection of the lettuce mosaic virus. The establishment of the method provides a new method for the rapid detection of the LMV.

Description

technical field [0001] The invention relates to the technical field of nano-enzyme immunochromatography. Specifically, it relates to a preparation method and a detection method of a lettuce mosaic virus nano-enzyme immunochromatographic test strip. Background technique [0002] Lettuce (Lactuca sativa), also known as lettuce, goose dish, etc., is a 1-year or 2-year-old herb of the genus Lactuca in the family Asteraceae, and is a common vegetable in life. Lettuce is vulnerable to viruses, and the viruses that infect lettuce mainly include lettuce mosaic virus (LMV), dandelion yellow mosaic virus (DaYMV), cucumber mosaic virus (CMV) and Tomato infertility virus (Tomato aspermyvirus, TAV) and other viruses. These viruses can infect and harm alone, and can also infect in combination of two or more. Lettuce is particularly susceptible to LMV infection. In the early stage of infection, irregular and shrunken leaf edges appear, followed by light green or light yellow mottled or ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/56983G01N33/54346Y02A50/30
Inventor 张永江符娜刘键魏梅生段德民向均
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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