Kit for detecting folic acid related gene polymorphism and using method thereof

A gene polymorphism and kit technology, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as expensive instruments, inability to determine the location and type of SNP, and restrictions on development. , to achieve the effect of simple operation, improved specificity and reduced pollution

Pending Publication Date: 2020-01-14
苏州云泰生物医药科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantages are also obvious. For example, whether there is a mutation can be judged, and the position and type of SNP cannot be determined. It needs to be verified by standard samples or combined sequencing, and the equipment is expensive, which also restricts the clinical development.

Method used

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  • Kit for detecting folic acid related gene polymorphism and using method thereof
  • Kit for detecting folic acid related gene polymorphism and using method thereof
  • Kit for detecting folic acid related gene polymorphism and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] A test kit for the detection of folic acid-related gene polymorphisms, the composition of which is shown in Table 6:

[0099] Table 6

[0100]

[0101]

[0102] The packaging and content of each component of the kit are shown in Table 7:

[0103] Table 7

[0104]

[0105]

Embodiment 2

[0106] Embodiment 2: the accuracy detection of the kit of this application

[0107] Adopt the test kit of embodiment 1 to detect the accuracy reference product:

[0108] Wherein, the preparation method of accuracy reference product is as shown in Table 8:

[0109] Table 8

[0110]

[0111] The prepared accuracy reference product is detected using the reaction solution, and the experimental results obtained are shown in Table 9:

[0112] Table 9

[0113]

[0114]

[0115] From the results in Table 9, it can be seen that the positive coincidence rate was 100% when testing the accuracy reference samples containing different genotypes respectively.

Embodiment 4

[0116] Example 4: Precision detection of the kit of the present application

[0117] The test kit of Example 1 is used to detect the precision reference product: wherein, the preparation method of the precision reference product is as shown in Table 10:

[0118] Table 10

[0119]

[0120] 10 holes will be prepared for each batch of the precision reference product, and the experimental results obtained are shown in Table 11 and Table 12:

[0121] Table 11

[0122]

[0123]

[0124] Table 12

[0125]

[0126] From the results in Tables 11 and 12, it can be seen that for each precision reference product, it can be detected after repeated 10 times in the corresponding reaction solution, and the CV values ​​of the experimental data are all less than 10%.

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Abstract

The invention relates to the field of gene polymorphism detection, in particular to a kit for detecting folic acid related gene polymorphism and a using method thereof. The kit includes a nucleic acidamplification reagent, and the nucleic acid amplification reagent includes a folic acid wild-type reaction solution, a folic acid mutant-type reaction solution and a mixed enzyme solution. The folicacid wild-type reaction solution contains primers and probes for detecting wild-type genes, and the folic acid mutant-type reaction solution contains primers and probes for detecting mutant-type genes. The kit has the technical advantages of high sensitivity, high specificity and good stability.

Description

technical field [0001] The present application relates to the field of gene polymorphism detection, in particular, to a kit for detecting folic acid-related gene polymorphisms and a method for using the same. Background technique [0002] Folic acid is an essential nutrient for the human body. Its main biological function is to participate in the methylation reaction in cells and the synthesis of deoxyribonucleic acid as a methyl donor. Folic acid cannot be directly used by the human body and must be activated through a series of activation Its physiological function, and folic acid ingested from food can only participate in many important biochemical metabolic reactions of the human body when it is reduced to tetrahydrofolate under the action of folate reductase. [0003] Folic acid deficiency is one of the environmental factors leading to abnormal embryonic development. In recent years, the relationship between folic acid metabolism and birth defects and adverse pregnancy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 谢立群李云航熊慧龚伟童光铨
Owner 苏州云泰生物医药科技有限公司
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