Enhanced Anti-Hair Loss Growth Composition
A technology for preventing hair loss and regenerating, the composition is applied in the directions of drug combination, hair care, medical preparations containing active ingredients, etc., and can solve the problems of high surgical treatment cost, drug dependence, side effects and the like
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Embodiment 1
[0030] Example 1 : Inhibition of 5α-reductase activity
[0031] In this example, different times of concentrated birch sap, ginger extract, Polygonum multiflorum root extract, ginseng extract, green tea extract, oriental orientalis extract, and their compositions on 5α-reductase activity were investigated and compared. Impact.
[0032] 1. Concentration of birch sap
[0033] The fresh birch sap stock solution purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. was input into the low-temperature drying equipment, cooled to -65°C, evacuated to 0.1Pa, and concentrated 1.5 and 10 times.
[0034] 2. Test
[0035] Experimental equipment: balance, wall breaker, constant temperature oscillator, high-speed refrigerated centrifuge, microplate reader.
[0036] Experimental reagents and consumables:
[0037] Finasteride: Shanghai Modern Pharmaceutical Co., Ltd. (tablet 5mg / tablet);
[0038] Reduced coenzyme II (NADPH): American Roche company (powder 10mg);
[0039] ...
Embodiment 2
[0060] Example 2 : Effects on the expression of genes and proteins related to hair loss prevention
[0061] In this example, the effects of different multiples of concentrated birch sap, angelica extract, astragalus, eucalyptus, birch bud extract, birch leaf extract, and their compositions on anti-hair loss and growth-related genes were investigated and compared. effect on protein expression.
[0062] 1. Concentration of birch sap
[0063] The fresh birch sap stock solution purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. was input into the low-temperature drying equipment, cooled to -65°C, evacuated to 0.1Pa, and concentrated 1.5 and 10 times.
[0064] 2. Test
[0065] The dermal papilla cells used in this experiment were produced by Guangdong Boxi Biotechnology Co., Ltd.
[0066] Experimental instrument: CO 2 Incubator (Thermo), ultra-clean workbench (Sujing Antai), inverted microscope (Olympus), micro shaker (Qilin Bell), microplate reader (BioTek)...
Embodiment 3
[0086] Example 3 : Effects on proliferation and differentiation of dermal papilla cells
[0087] In this example, the effects of different times of concentrated birch sap, adenosine, nicotinamide, copper tripeptide-1, and their combinations on the proliferation and differentiation ability of dermal papilla cells were investigated and compared.
[0088] 1. Concentration of birch sap
[0089] The fresh birch sap stock solution purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. was input into the low-temperature drying equipment, cooled to -65°C, evacuated to 0.1Pa, and concentrated 1.5 and 10 times.
[0090] 2. Test method
[0091] The dermal papilla cells used in this experiment were produced by Guangdong Boxi Biotechnology Co., Ltd.
[0092] Experimental instrument: CO 2 Incubator (Thermo), ultra-clean workbench (Sujing Antai), inverted microscope (Olympus), micro shaker (Qilin Bell), microplate reader (BioTek), incubator (Tester).
[0093] Experimental...
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