Animal model with cell-in-cell (CIC) structure and preparation method thereof

A technology of animal models and mouse models, applied in the direction of biochemical equipment and methods, preparation of test samples, preparations for in vivo experiments, etc., can solve the problems of staying, lack of reliable animal model support, etc.

Inactive Publication Date: 2020-01-31
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past ten years, the mechanism, formation rules, signaling pathways and biological functions of CIC-mediated cell death have gradually become clear, but the reported results are only at the level of in vitro cell experiments, lacking reliable animal model support

Method used

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  • Animal model with cell-in-cell (CIC) structure and preparation method thereof
  • Animal model with cell-in-cell (CIC) structure and preparation method thereof
  • Animal model with cell-in-cell (CIC) structure and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1, establishment of animal model with CIC structure in vivo

[0037] 1. Group processing

[0038] A total of 9 6-week-old healthy female C57BL / 6 mice were selected, weighing 20-22 g, with similar activity ability. Randomly divided into 3 groups (3 animals in each group):

[0039] The first group (control group): At the initial moment of the experiment (0 time), each mouse was intraperitoneally injected with 0.1 mL of sterile pyrogen-free PBS buffer; at 60 hours, each mouse was intraperitoneally injected with 0.1 mL of sterile pyrogen-free PBS buffer;

[0040] The second group (colchicine 1mM group): at the initial moment of the experiment (time 0), each mouse was intraperitoneally injected with 0.1mL 1mM colchicine solution; at 60 hours, each mouse was intraperitoneally injected with 0.1mL 1mM colchicine solution ;

[0041] The third group (colchicine 2mM group): At the initial moment of the experiment (time 0), each mouse was intraperitoneally injected w...

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Abstract

The invention discloses an animal model with a cell-in-cell (CIC) structure and a preparation method thereof. The invention protects application of a mitotic inhibitor in preparation of a kit; and thefunction of the kit is to prepare the animal model with the CIC structure. The invention also protects application of the mitotic inhibitor in preparation of the animal model with the CIC structure.The invention further protects a method for preparing an animal model. The method for preparing the animal model comprises the following step of administrating an animal with a mitotic inhibitor, thereby obtaining the animal model with the CIC structure. The invention still further protects application of an animal model prepared by any of the above methods, including: application of the animal model in CIC structure research, application of the animal model in screening of substances with tumor therapeutic functions, and application of the animal model in toxicological analysis of substancesto be tested to evaluate biosafety of the substance to be tested. For the first time, the invention discloses a preparation method by which the CIC structure is successfully induced in an animal body;and therefore, a favorable experimental basis is provided for further exploring of physiological and pathological significance of CIC.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and relates to a cell-in-cell structure animal model and a preparation method thereof. Background technique [0002] Cell death is an important life process that plays a key role in the whole growth and development of organisms. Cell death mainly includes cell necrosis and programmed cell death. Cell necrosis refers to a kind of passive cell death, which is characterized by cell swelling, cell membrane damage, release of endolytic substances, and causes obvious local inflammatory response in the body. Different from cell necrosis, programmed cell death is regulated by specific genes, and is an important regulatory mechanism for organisms to maintain homeostasis and normal tissue functions. Apoptosis belongs to the main category of programmed cell death, which is mediated by a series of cysteine-containing aspartic acid proteolytic enzymes caspase, which disintegrates cells by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/165A61K31/4184A61K31/475A61K31/407A61K31/337A61K49/00C12Q1/6876C12Q1/02G01N1/30G01N21/01
CPCA61K31/165A61K31/337A61K31/407A61K31/4184A61K31/475A61K45/00A61K49/0008C12Q1/6876G01N33/5005
Inventor 孙强牛祖彪梁剑青张波
Owner ACADEMY OF MILITARY MEDICAL SCI
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