Method for non-labeled homogeneous phase cathode photoelectrochemical detection of 17[Beta]-estradiol

A photoelectrochemical and estradiol technology, applied in the field of analysis and detection technology and analysis and detection, can solve problems such as low cost and reduced activity

Active Publication Date: 2020-02-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The cathodic photoelectrochemical detection method solves the following problems well: (1) There is no need to immobilize biomolecules on the electrode surface, and the reaction is carried out in a homogeneous solution. The subsequent activity is reduced; (2) No need to label biomolecules, and the cost is low

Method used

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  • Method for non-labeled homogeneous phase cathode photoelectrochemical detection of 17[Beta]-estradiol
  • Method for non-labeled homogeneous phase cathode photoelectrochemical detection of 17[Beta]-estradiol
  • Method for non-labeled homogeneous phase cathode photoelectrochemical detection of 17[Beta]-estradiol

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Experimental program
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Effect test

Embodiment 1

[0024] a. CuBi 2 o 4 Preparation of cathode semiconductor material: First, 0.24g of Bi(NO 3 ) 3 ·5H 2 O was dissolved homogeneously in 10mL deionized water containing 0.35mL concentrated nitric acid and stirred thoroughly; mixed with 5mL 5mmol / L CuSO 4 After the solution is mixed, slowly drop 7mL of 1.0mol / L NaOH solution into the mixed solution until a blue-green precipitate is produced, then transfer it to the reaction kettle, and keep it at 120°C for 20h; take it out, wash it by centrifugation and dry it for later use;

[0025] b. CuBi 2 o 4 Preparation of modified ITO electrode: the prepared CuBi 2 o 4 The solid powder is made into a 1mg / mL solution, dropped on the surface of the pretreated ITO conductive glass, and after natural drying, CuBi can be obtained 2 o 4 Modified ITO electrode;

[0026] c. Determination of 17β-estradiol: Mix different concentrations of 17β-estradiol and 5 μL 10 μmol / L 17β-estradiol aptamer probe HP1 with 200 mmol / L NaCl, 25 mmol / L KCl, ...

Embodiment 2

[0028] a. CuBi 2 o 4 Preparation of cathode semiconductor material: First, 0.24g of Bi(NO 3 ) 3 ·5H 2 O was homogeneously dissolved in 10 mL of deionized water containing 0.35 mL of concentrated nitric acid and stirred thoroughly; mixed with 5 mL of 5 mmol / L Cu(NO 3 ) 2 After the solution is mixed, slowly drop 7mL of 1.0mol / L NaOH solution into the mixed solution until a blue-green precipitate is produced, then transfer it to the reaction kettle, and keep it at 120°C for 22 hours; take it out, wash it by centrifugation and dry it for later use;

[0029] b. CuBi 2 o 4 Preparation of modified ITO electrode: the prepared CuBi 2 o 4 The solid powder is made into a 1mg / mL solution, dropped on the surface of the pretreated ITO conductive glass, and after natural drying, CuBi can be obtained 2 o 4 Modified ITO electrode;

[0030] c. Determination of 17β-estradiol: Mix different concentrations of 17β-estradiol and 5 μL 10 μmol / L 17β-estradiol aptamer probe HP1 with 200 mmol...

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Abstract

The invention relates to a novel cathode photoelectrochemical method for homogeneous phase detection of 17[Beta]-estradiol using a CuBi<2>O<4> material, and belongs to the field of analysis and detection. An ITO electrode modified by CuBi<2>O<4> is used as a working electrode, methylene blue (MB) or ferric chloride hemin that can be embedded in G-tetrahedra are used as photoelectrochemical signalmolecules, and the specific binding reaction between 17[Beta]-estradiol and aptamers is combined with the rolling circle amplification reaction (RCA) to control the insertion / release of the signal molecules, so that a homogeneous phase cathode photoelectrochemical detection method without biomolecule labeling and without fixing the biomolecules on the electrode surface is constructed. The novel cathode photoelectrochemical method for homogeneous phase detection of 17[Beta]-estradiol using the CuBi<2>O<4> material has high sensitivity and selectivity, with a linear range of 0.05 nmol / L - 10 nmol / L and a detection limit of 2.0 pmol / L.

Description

technical field [0001] The invention relates to an analysis and detection technology, belonging to the technical field of analysis and detection. Background technique [0002] Describe the status and existing problems of the prior art closest to the present invention. [0003] In the production process of animal husbandry and cosmetic industry, the abuse of hormones can lead to antibiotic contamination and hormone residues in meat, eggs, dairy products and cosmetics [Liu, Y.; D, G.S.; Li, P.; Fang, D.Y. Huang, S.S. Chin J Anal Chem, 2014, 42, 623–628; Giusti, R.M.; Iwamoto, K.; Hatch, E.E. Annn Intern. Med. 2009, 122:778-788; Liu, X.G. These hormones, especially estrogens such as 17β-estradiol, can cause endocrine-related tumors in the body and defects in newborn babies, and can also cause damage to the liver and kidneys, and have a serious impact on human growth [Hadjimichael, O.C.; Meigs, J.W.J. Natl. Cancer Inst. 1984, 73:831-834; Arnold, S, F.; Klotz, D.M.; Collins, B....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/531C12Q1/6844G01N27/327G01N27/416
CPCC12Q1/6844G01N27/3275G01N27/416G01N33/531G01N33/74C12Q2531/125C12Q2521/501
Inventor 王光丽顾萌萌孙冬雪吴秀明刘田利
Owner JIANGNAN UNIV
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