Nucleic acid detection device, method and system

A detection device and nucleic acid technology, applied in the detection field, can solve problems such as nucleic acid extraction constant temperature amplification reaction melting test, etc., to achieve stable detection, reduce operating procedures, and improve detection efficiency

Pending Publication Date: 2020-02-14
SHANGHAI IGENETEC DIAGNOSTICS CO LTD +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] However, there is no mention of an integrated solution for nucleic acid extraction, constant temperature amplification reaction, and melting test in the prior art

Method used

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  • Nucleic acid detection device, method and system
  • Nucleic acid detection device, method and system
  • Nucleic acid detection device, method and system

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Embodiment Construction

[0048] Various exemplary embodiments, features, and aspects of the present disclosure will be described in detail below with reference to the accompanying drawings. The same reference numbers in the figures indicate functionally identical or similar elements. While various aspects of the embodiments are shown in drawings, the drawings are not necessarily drawn to scale unless specifically indicated.

[0049] The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration." Any embodiment described herein as "exemplary" is not necessarily to be construed as superior or better than other embodiments.

[0050]In addition, in order to better illustrate the present disclosure, numerous specific details are given in the following specific implementation manners. It will be understood by those skilled in the art that the present disclosure may be practiced without some of the specific details. In some instances, methods, means, component...

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Abstract

The invention relates to a nucleic acid detection device, method and system. The device comprises a microfluidics module, a temperature control area, a rotation module, a temperature control module and a detection module, wherein the microfluidics module comprises a microfluidic chip; the temperature control area is arranged below the microfluidic chip and comprises a first temperature area, a second temperature area, a thermal insulation area and detection holes; the rotation module is connected to the microfluidic chip; the temperature control module is connected to the temperature control area; and the detection module is used for detecting a fluorescence signal generated by samples in reaction holes through the detection holes, and a detection result is output. Through the cooperationof each module of the nucleic acid detection device, according to the nucleic acid detection device of the embodiment of the invention, nucleic acid purification and nucleic acid detection integrationcan be realized, operation flows are reduced, and therefore, working efficiency is improved.

Description

technical field [0001] The present disclosure relates to the technical field of detection, and in particular to a nucleic acid detection device, method and system. Background technique [0002] Microfluidics integrates the basic operation units such as sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis processes into a micron-scale chip to automatically complete the entire analysis process. Microfluidic Chip (Microfluidic Chip) is currently a hot field in the development of Micro Total Analysis Systems (Miniaturized Total Analysis Systems). [0003] The traditional nucleic acid detection technology is currently based on PCR (English: Polymerase Chain Reaction, Chinese: Polymerase Chain Reaction), which requires rapid switching between different temperatures to realize the cycle of denaturation, annealing, and extension functions. Switching between temperatures requires high equipment and takes a long time, resulting in ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/00C12Q1/6844
CPCB01L7/5255C12Q1/6844C12Q2527/101C12Q2565/629C12Q2563/107
Inventor 方雪恩杨波朱美娇王耀华孔继烈
Owner SHANGHAI IGENETEC DIAGNOSTICS CO LTD
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